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From 2014.igem.org

21^st August

• Chewback: purification of RFP (706pb) and ELP (6240 pb)
  

  Phusion polymerase	0,5μl
  Buffer 5X GC	10μl
  dNTP 10mM	1μl
  primer ELP reverse	2,5μl
  primer ELP forward	2,5μ

  Gel 1% (30min, 100v) : no results

• Problem with preculture : Test of ampicillin, medium and bacteria

• We change the water of dialysis at 9h30 and 14h10 and 19h20