From 2014.igem.org

• 
• 
• 
• 
• 
• 
• 
• 
• 
• 
• 
• 
• 

13^th August

• Efficiency test
  Results : 2x104 => failure
• Making competent cells
• Transformation and ligation
  With daily competent BL21.
  => Spread on LB+chlo plates.