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From 2014.igem.org

- Marchantia Growth You will need a room/compartment at room temperature under full illumination. Ventilation is also recommended, but not essential

Transformation For transformation using cocultivation, you will need a shaker under full illumination capable of shaking 200ml conical flasks.

Otherwise, a ventilated illumination space at room temperature is sufficient.

Other Porous tape to seal plates from fungal contamination, but enabling gas exchange, is essential

-Agrobacteria Growth You will need a 30degree incubator, as well as a room temperature shaker for growing liquid cultures.

Transformation You will need electroporation equipment capable of delivering at least 1.25kV (if using 1mm cuvettes)