Team:Warwick/Project

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      <h1 class="title" align="center">The Project</h1>
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            <li> <a href = "/Team:Warwick"> HOME </a> </li>
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            <li> <a href = "/Team:Warwick/Project"> <span> PROJECT </span> </a> </li>
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            <li> <a href = "/Team:Warwick/Team"> TEAM </a> </li>
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            <li> <a href = "/Team:Warwick/Parts"> PARTS </a> </li>
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<li> <a href = "/Team:Warwick/Project/Motivation"> MOTIVATION </a> </li>
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<li> <a href = "/Team:Warwick/Project/Applications"> APPLICATIONS </a> </li>
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<h2>WELCOME TO RNA WORLD</h2>
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<p> Welcome to the biological world which we have spent an entire summer creating and perfecting. We are introducing RNA to synthetic biology in a big way by directly contributing a number of key parts to iGEM.  We have taken a risk, we have been told our project is unrealistic and should be scrapped, that a group of undergraduates are crazy for even thinking about doing something so complicated. At times, we doubted ourselves- there have been good times and bad times.  Spurred on by a desire to make a difference to synthetic biology and to the world in which we live, we pressed forward - a new team to iGEM, not seasoned veterans. We take chances and risks to make a difference - whether success comes our way or failure, we embrace the moment, cherishing it. We hope that the synthetic biology community will appreciate our efforts, as RNA usage and popularity surges. DNA best serves as a template for information storage whereas RNA is multi-functional, catalytic and dynamic. Welcome to our world - the RNA world!<br>
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<b>- Warwick iGEM Team 2014 </b>
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        The Sheffield iGEM 2014 team have chosen to address the challenge of reducing the amount of fats, oils and greases (FOGs) entering the sewage system. These contribute to major costs and blockages in the systems known as <a href="http://tinyurl.com/mdhrfmk"><i>fatbergs</i> </a>. At present, there are over 200,000 major UK sewage blockages per year, of which <sup>3</sup>/<sub>4</sub> are caused by FOGs, meaning this is a major challenge to infrastructure and clearly a global challenge as cities expand, particularly in developing countries. These drain blockages in the UK are a growing problem costing Thames Water (London) alone, £1 million per month to tackle. In addition to FOGs, human hair is a well-known problem in wastewater treatment, being among a range of difficult to degrade materials.
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      <h2>The Concept</h2>
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        Objectively, the team aim to build a genetically engineered, non-pathogenic strain of E. coli to responsively secrete lipase and keratinase in the presence of fats and hair in order to degrade and solubilise the components; this will ultimately prevent the blockages in the network. This will then be developed further in the future, by incorporating a transport system into the bacteria to uptake the lipid components to harvest and reuse as a potential energy source.
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      <h2>The Parts</h2>
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        The system being created in E. coli is composed of several key BioBrick parts from the iGEM registry:
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            <td>BBa_K258006</td>
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            <td>Thermostable lipase (TliA) of <i>P. fluorescens</i> SIK W1</td>
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            <td>The TliA lipase is secreted using an ABC transporter to degrade fats; it is thermostable and therefore efficient when functioning at high temperatures, such as within the water network.</td>
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            <td>BBa_K258008</td>
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            <td>ABC transporter of <i>E. chrysanthemi</i></td>
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            <td>A type I secretion system used to export proteins with C-terminal fragments, such as TliA</td>
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            <td>BBa_K861060</td>
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            <td>PfadR promoter</td>
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            <td>An artificial promoter containing a tandem FadR binding site which is not affected by glucose concentration, oxidative stress and several other factors.</td>
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            <td>BBa_K861061</td>
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            <td>RFP generator of PfadR</td>
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            <td>When placed downstream of K861060, efficacy of the promoter can be tested by allowing visualisation of red fluorescence protein.</td>
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            <td>Composite used with BBa_K258008</td>
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            <td>This construct allows production of a promoter, RBS and lipase ABC transporter recognition domain to allow lipid secretion outside of the cell.</td>
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            <td>Lipase ABC transporter recognition domain</td>
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            <td>This domain will bind the lipase to allow secretion.</td>
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          <td>pLac + RBS + Secretion Signal and Streptavidin Binding Tags</td>
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          <td>This generator has a promoter which is IPTG-inducible and a strong RBS to allow expression of our transporter.</td>
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        Alongside these existing parts, we are developing our own keratinase enzyme part, which we will submit to the registry as a new, documented BioBrick.
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      <h2>The Approach</h2>
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        Alongside the laboratory work to construct this system, the team are collaborating with the water industry and potential users of the system in order to develop an implementation device for its application in society. The broader impact of this device will then be considered from many different aspects to evaluate the product. As well as this, outreach activities within and outside of the University to promote synthetic biology will be carrier out. These aim to deepen understanding of the new technology and gauge interest and support to allow successful incorporation of it into society.
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  <p>Created by: iGEM Warwick</p>
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            <div class="caption"><h1>Ben Madden</h1><p>Hi I'm Ben, a third year Chemical Engineer</p>
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            <div class="caption"><h1>Sharan Nanuan</h1><p>Hi I'm Sharan, a second year MBB student</p>
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        ERASynBio promotes the robust development of Synthetic Biology by structuring and coordinating national efforts and investment. They have developed a strategic vision to support emergence of Synthetic Biology programmes to lay the ground for transnational funding activities via joint calls in the project. They stimulate and tackle the interdisciplinary nature and immaturity by offering training and educational possibilities, establishing an interdisciplinary advisory board and inviting observers of other funding organisations.
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Latest revision as of 03:40, 18 October 2014

WELCOME TO RNA WORLD

Welcome to the biological world which we have spent an entire summer creating and perfecting. We are introducing RNA to synthetic biology in a big way by directly contributing a number of key parts to iGEM. We have taken a risk, we have been told our project is unrealistic and should be scrapped, that a group of undergraduates are crazy for even thinking about doing something so complicated. At times, we doubted ourselves- there have been good times and bad times. Spurred on by a desire to make a difference to synthetic biology and to the world in which we live, we pressed forward - a new team to iGEM, not seasoned veterans. We take chances and risks to make a difference - whether success comes our way or failure, we embrace the moment, cherishing it. We hope that the synthetic biology community will appreciate our efforts, as RNA usage and popularity surges. DNA best serves as a template for information storage whereas RNA is multi-functional, catalytic and dynamic. Welcome to our world - the RNA world!
- Warwick iGEM Team 2014



Retrieved from "http://2014.igem.org/Team:Warwick/Project"