Team:Warwick/Interlab
From 2014.igem.org
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+ | <li style="list-style-type: none;"><a href="#Introduction"><span class="tocnumber"> 1. </span><span class="toctext"> Introduction </span></a></li> | ||
+ | <li style="list-style-type: none;"><a href="#Timeline"><span class="tocnumber">2. </span><span class="toctext"> The experimental timeline </span></a></li> | ||
+ | <li style="list-style-type: none;"><a href="#Protocols"><span class="tocnumber">3. </span><span class="toctext"> Protocols and methodology </span></a></li> | ||
+ | <li style="list-style-type: none;><a href="#Measurements"><span class="tocnumber">4. </span><span class="toctext"> Measurements and results </span></a></li> | ||
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+ | <h1 id="Introduction"> Introduction </h1> | ||
<p> I read the interlab study as an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose value for other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. Although its motivations are shrouded in mystery, I percieve the interlab study as the first step towards that ultimate goal of blanket standardisation in a synthetic biological context. </p> | <p> I read the interlab study as an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose value for other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. Although its motivations are shrouded in mystery, I percieve the interlab study as the first step towards that ultimate goal of blanket standardisation in a synthetic biological context. </p> | ||
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<p> It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate two members of the team to pursuing it in parallel to our other endeavours, as a sort of side quest. </p> | <p> It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate two members of the team to pursuing it in parallel to our other endeavours, as a sort of side quest. </p> | ||
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Timeline of when protocols were run and measurements were taken is listed below | Timeline of when protocols were run and measurements were taken is listed below | ||
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<li> 28/08/2014 Collected data from plate reader and imported into Excel for analysis </li> | <li> 28/08/2014 Collected data from plate reader and imported into Excel for analysis </li> | ||
- | < | + | <p> The remit of the interlab study boils down to constructing and characterising, albeit minimally, three devices. They share a lot of similarities, and the objective is obviously not to create some wacky new form of life, but to measure well characterised and well understood parts in order to measure the measuring equipment, as it were. I will quickly describe the nature of these devices. </p> |
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+ | <h1 id="Protocols"> Protocols and methodology </h1> | ||
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<h7> Many of the protocols mentioned above which we used were harvested straight from the iGEM website, but we also used content from previous iGEM teams and instructions packaged with kits. The specific materials and procedures can be accessed through clicking the relevant hyperlink </h7> | <h7> Many of the protocols mentioned above which we used were harvested straight from the iGEM website, but we also used content from previous iGEM teams and instructions packaged with kits. The specific materials and procedures can be accessed through clicking the relevant hyperlink </h7> | ||
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<li> <a href="https://2010.igem.org/Team:Newcastle/Gel_electrophoresis">Gel electrophoresis </a></li> | <li> <a href="https://2010.igem.org/Team:Newcastle/Gel_electrophoresis">Gel electrophoresis </a></li> | ||
<li> 3A Assembly basically comprises digestion and ligation </li> | <li> 3A Assembly basically comprises digestion and ligation </li> | ||
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+ | <h1 id="Measurements"> Measurements and results </h4> | ||
Revision as of 09:17, 16 October 2014
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Introduction
I read the interlab study as an attempt by iGEM HQ to conduct a comparative analysis of the different methods employed by the varied and diverse teams internationally to arrive at useful data. A key problem in science is ascertaining absolute measurements; there is no point in one measuring the fluorescence of some given part, only to arrive at arbitrary units whose value for other scientists is near zero. Standards must be set, in order to embue our results with any useful meaning. Although its motivations are shrouded in mystery, I percieve the interlab study as the first step towards that ultimate goal of blanket standardisation in a synthetic biological context.
It is suggested that a team conduct the interlab study as a preamble to the main event. However, we are the first example of an iGEM team at Warwick, and coalesced rather late in the day. Hence we decided just to dedicate two members of the team to pursuing it in parallel to our other endeavours, as a sort of side quest.
The experimental timeline
Timeline of when protocols were run and measurements were taken is listed belowThe remit of the interlab study boils down to constructing and characterising, albeit minimally, three devices. They share a lot of similarities, and the objective is obviously not to create some wacky new form of life, but to measure well characterised and well understood parts in order to measure the measuring equipment, as it were. I will quickly describe the nature of these devices.