Team:UCL/Science/Sequences

From 2014.igem.org

Revision as of 19:29, 12 October 2014 by Sanjaycj (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Goodbye Azodye UCL iGEM 2014

Gene and BioBrick Part Sequences

Laboratory Team

Summary

For ease of reference, the sequences for (1) our BioBrick Gene Designs, (2) Plasmid / Vector Backbones, and (3) BioBricks that we have used from that iGEM Registry Distribution, have been provided below. Clicking on the Gene/Plasmid/BioBrick headers will take you to pages describing the part in more detail and the source of the sequences.

Note: format is a little horrendous, will change to all CAPS, and at a set width (70-75 char), and add "br" for all later.

BioBrick Designs for Our Genes

[New] BBa_K1336000 / AzoR Part-only sequence (612bp) [Link, see DT]

Sequence taken from Lisbon.

ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGT CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA 
AGC GTT GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT GCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC 
GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GCC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC 
GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG TGC GGC AAC AAG 
AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CTG CAC GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GTG TTC CTG GGC TTC ATC GGG 
ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GCC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATC GAT GCT GCA CAG GCG CAG ATT GCC AGC GAG 
CTG TTT GCG GCT GCC TGA

[New] BBa_K1336001 / AzoR 1B6 (heat-stable AzoR mutant) Part-only sequence (612bp) [Link, see DT]

Sequence given from Lisbon group directly.

ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGC CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA 
AGC GTG GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT CCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC 
GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GGC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC 
GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG AGC GGC GAC AAG 
AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CAG CAT GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GCG TTC CTG GGC TTC ATC GGG 
ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GAC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATA GAT GCT GCA CGG GCG CAG ATT GCC AGC GAG 
CTG TTT GCG GCT GCC TGA

[Proposed] BBa_K1336002 / CotA Part-only sequence (1542bp) [Link, see DT]

Sequence given from Lisbon group directly.

ATG ACA CTT GAA AAA TTT GTG GAT GCT CTC CCA ATC CCA GAT ACA CTA AAG CCA GTA CAG CAA TCA AAA GAA AAA ACA TAC TAC GAA GTC ACC ATG GAG 
GAA TGC ACT CAT CAG CTC CAT CGC GAT CTC CCT CCA ACC CGC CTG TGG GGC TAC AAC GGC TTA TTT CCG GGA CCG ACC ATT GAG GTT AAA AGA AAT GAA 
AAC GTA TAT GTA AAA TGG ATG AAT AAC CTT CCT TCC ACG CAT TTC CTT CCG ATT GAT CAC ACC ATT CAT CAC AGT GAC AGC CAG CAT GAA GAG CCC GAG 
GTA AAG ACT GTT GTT CAT TTA CAC GGC GGC GTC ACG CCA GAT GAT AGT GAC GGG TAT CCG GAG GCT TGG TTT TCC AAA GAC TTT GAA CAA ACA GGA CCT 
TAT TTC AAA AGA GAG GTT TAT CAT TAT CCA AAC CAG CAG CGC GGG GCT ATA TTG TGG TAT CAC GAT CAC GCC ATG GCG CTC ACC AGG CTA AAT GTC TAT 
GCC GGA CTT GTC GGT GCA TAT ATC ATT CAT GAC CCA AAG GAA AAA CGC TTA AAA CTG CCT TCA GAC GAA TAC GAT GTG CCG CTT CTT ATC ACA GAC CGC 
ACG ATC AAT GAG GAT GGT TCT TTG TTT TAT CCG AGC GCA CCG GAA AAC CCT TCT CCG TCA CTG CCT AAT CCT TCA ATC GTT CCG GCT TTT TGC GGA GAA 
ACC ATA CTC GTC AAC GGG AAG GTA TGG CCA TAC TTG GAA GTC GAG CCA AGG AAA TAC CGA TTC CGT GTC ATC AAC GCC TCC AAT ACA AGA ACC TAT AAC 
CTG TCA CTC GAT AAT GGC GGA GAT TTT ATT CAG ATT GGT TCA GAT GGA GGG CTC CTG CCG CGA TCT GTT AAA CTG AAT TCT TTC AGC CTT GCG CCT GCT 
GAA CGT TAC GAT ATC ATC ATT GAC TTC ACA GCA TAT GAA GGA GAA TCG ATC ATT TTG GCA AAC AGC GCG GGC TGC GGC GGT GAC GTC AAT CCT GAA ACA 
GAT GCG AAT ATC ATG CAA TTC AGA GTC ACA AAA CCA TTG GCA CAA AAA GAC GAA AGC AGA AAG CCG AAG TAC CTC GCC TCA TAC CCT TCG GTA CAG CAT 
GAA AGA ATA CAA AAC ATC AGA ACG TTA AAA CTG GCA GGC ACC CAG GAC GAA TAC GGC AGA CCC GTC CTT CTG CTT AAT AAC AAA CGC TGG CAC GAT CCC 
GTC ACA GAA ACA CCA AAA GTC GGC ACA ACT GAA ATA TGG TCC ATT ATC AAC CCG ACA CGC GGA ACA CAT CCG ATC CAC CTG CAT CTA GTC TCC TTC CGT 
GTA TTA GAC CGG CGG CCG TTT GAT ATC GCC CGT TAT CAA GAA AGC GGG GAA TTG TCC TAT ACC GGT CCG GCT GTC CCG CCG CCG CCA AGT GAA AAG GGC 
TGG AAA GAC ACC ATT CAA GCG CAT GCA GGT GAA GTC CTG AGA ATC GCG GCG ACA TTC GGT CCG TAC AGC GGA CGA TAC GTA TGG CAT TGC CAT ATT CTA 
GAG CAT GAA GAC TAT GAC ATG ATG AGA CCG ATG GAT ATA ACT GAT CCC CAT AAA TAA

[New] BBa_K1336003 / BsDyP Part-only sequence (1251bp) [Link, see DT]

Sequence given from Lisbon group directly.

ATG AGC GAT GAA CAG AAA AAG CCA GAA CAA ATT CAC AGA CGG GAC ATT TTA AAA TGG GGA GCG ATG GCG GGG GCA GCC GTT GCG ATC GGT GCC AGC GGT 
CTC GGC GGT CTC GCT CCG CTT GTT CAG ACT GCG GCT AAG CCA TCG AAA AAG GAT GAA AAA GAG GAG GAG CAG ATT GTT CCG TTT TAC GGA AAG CAT CAG 
GCC GGA ATC ACA ACT GCC CAT CAG ACG TAT GTC TAT TTT GCT GCA CTG GAT GTA ACA GCA AAA GAC AAA AGC GAC ATC ATT ACG TTA TTT AGA AAC TGG 
ACA AGT CTC ACA CAG ATG CTG ACG TCT GGC AAG AAA ATG TCA GCG GAG CAG AGA AAT CAG TAT CTG CCG CCG CAG GAT ACA GGT GAG TCT GCT GAT TTA 
TCC CCT TCC AAC TTA ACG GTC ACA TTC GGA TTC GGG CCT GGC TTT TTT GAA AAA GAC GGA AAG GAC CGC TTC GGA CTG AAA AGC AAA AAA CCG AAG CAC 
CTA GCC GCT CTT CCA GCG ATG CCC AAT GAC AAC CTG GAT GAA AAG CAG GGA GGC GGG GAC ATC TGC ATT CAA GTG TGT GCA GAC GAC GAG CAA GTG GCG 
TTT CAC GCG CTG CGT AAC CTG CTA AAT CAA GCG GTC GGC ACT TGT GAG GTC CGT TTT GTG AAC AAA GGC TTT TTA AGC GGT GGA AAA AAT GGT GAA ACA 
CCG CGC AAC CTC TTC GGG TTT AAA GAT GGA ACA GGC AAC CAG AGC ACG AAG GAT GAC ACG TTG ATG AAC TCG ATC GTG TGG ATT CAG TCT GGT GAG CCT 
GAC TGG ATG ACG GGC GGC ACC TAT ATG GCT TTT CGG AAA ATC AAA ATG TTC CTT GAG GTA TGG GAC CGC TCT TCC CTC AAG GAC CAA GAG GAT ACC TTC 
GGC CGC AGA AAA AGC TCG GGG GCG CCT TTT GGG CAG AAA AAG GAA ACA GAT CCC GTG AAG CTG AAT CAA ATT CCG TCT AAT TCA CAC GTC AGT CTA GCG 
AAA TCT ACT GGG AAA CAA ATT TTG CGA AGA GCT TTC TCT TAC ACA GAA GGA CTT GAT CCG AAA ACC GGC TAT ATG GAT GCA GGT CTC CTG TTT ATC AGT 
TTT CAA AAA AAT CCC GAC AAT CAG TTT ATC CCG ATG CTG AAG GCT CTT TCA GCA AAG GAT GCG TTA AAC GAA TAT ACA CAA ACA ATC GGT TCT GCT TTA 
TAC GCA TGC CCA GGC GGC TGC AAA AAA GGA GAA TAT ATT GCC CAG CGT TTG CTG GAA TCA TAA

[Proposed] BBa_K1336004 / PpDyP Part-only sequence (864bp) [Link, see DT]

Sequence taken from Lisbon.

ATG CCG TTC CAG CAA GGT CTG CTT GCC ACC CCG GTG CCG GCC CAC GCC CGT CAC CTG TTC TTC ACC CTG CAG TCG CCC GAG GCG TTG CCT GCC GCG CTG 
GAT GCC TTG CTG CCG CAG GTC GAT GGC AAA CAG TTG CTG CTG GGC GTA GGC GCC CCC TTG GCC AAG GCC CTG GGC CGG GAA ATA CCA GGC CTG CGC CCG 
TTC CCG CTA CTG GAT GCC GCG GTT GAG AAC CCC AGC ACC CAA CAC GCC CTG TGG CTG TGG CTG CGT GGC GAC GAA CGT GGC GAC CTG CTG CTG CGC ACC 
CAG GCC CTG GAG CAG GCG CTG GCA CCC GCC CTG AGC CTG GCC GAC AGC GTT GAT GGC TTC CTG CAC CGG GGC GGC CAT GAC CTT ACC GGT TAT GAA GAC 
GGC ACC GAA AAC CCG ACA GAC GAA GAG GCG GTG CAA GCG GCC ATT GCC GCA GAT GGC TCC AGC TTT GCC GCG TTC CAG CTG TGG AAG CAC GAC CTG CAA 
TAC TTC AAG TCG CTG CCC CAG GCA GAC CAG GAC AAC ATC ATC GGC CGT CGC TTG AGC GAT AAC GAA GAA CTC GAC GAC GCC CCC GCG TCC GCG CAC GTC 
AAG CGC ACC GCC CAG GAA AGC TTT GAA CCC GAG GCG TTC ATG GTC CGT CGC TCG GTA TCC TGG GCC GAC CAA CGC GGC GCC GGC CTG GCT TTC GTC GCC 
CTG GGC AAG AGT TTC GAG GCA TTC GAG GTG CAG CTG CGG CGC ATG AGT GGC CTG GAA GAC GGC ATC ATC GAC GGG CTG TAC CGC TTC AGC CGC CCG CTG 
ACG GGG GGC TAC TAC TGG TGC CCG CCA ATG AGC GAG ACG GGT GTC GAT CTA AGC CCC TTG CTG CGG GCC TGA

[Improvement] BBa_K500000 / LiP Part-only sequence (1116bp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.

ATG GCT TTT AAA CAA TTA TTC GCC GCT ATT TCC TTG GCT TTG TCT TTG TCT GCT GCT AAT GCT GCT GCT GTT ATT GAA AAA AGA GCT ACT TGT TCC AAC 
GGT AAG ACT GTT GGT GAT GCT TGT TCT TGT GCT TGG TTT GAT GTT TTG GAC GAC ATC CAA CAA AAC TTG TTT CAT GGT GGT CAA TGT GGT GCT GAA GCT 
CAT GAA TCT ATC AGA TTG GTT TTC CAT GAC TCC ATT GCT ATT TCT CCA GCT ATG GAA GCT CAA GGT AAA TTT GGT GGA GGT GGT GCT GAT GGT TCC ATT 
ATG ATT TTC GAT GAT ATC GAA ACC GCC TTC CAT CCA AAT ATT GGT TTG GAC GAA ATC GTC AAG TTG CAA AAG CCA TTT GTT CAA AAG CAC GGT GTT ACT 
CCA GGT GAT TTT ATT GCT TTT GCT GGT GCT GTT GCT TTG TCT AAT TGT CCA GGT GCT CCA CAA ATG AAT TTC TTT ACT GGT AGA GCA CCA GCT ACT CAA 
CCA GCT CCA GAT GGT TTG GTT CCA GAA CCA TTT CAT ACC GTT GAC CAA ATC ATC AAC AGA GTT AAT GAC GCC GGT GAA TTT GAC GAA TTG GAA TTG GTT 
TGG ATG TTG TCT GCT CAT TCT GTT GCT GCT GTT AAT GAT GTT GAT CCA ACT GTT CAA GGT TTG CCA TTT GAT TCT ACC CCA GGT ATT TTC GAT TCC CAA 
TTC TTC GTT GAA ACC CAA TTG AGA GGT ACT GCT TTT CCA GGT TCT GGT GGT AAT CAA GGT GAA GTT GAA TCT CCA TTG CCA GGT GAA ATT AGA ATC CAA 
TCC GAT CAT ACC ATT GCC AGA GAT TCA AGA ACT GCT TGT GAA TGG CAA TCC TTC GTT AAC AAT CAA TCC AAG TTG GTT GAC GAC TTC CAA TTC ATT TTC 
TTG GCT TTG ACT CAA TTG GGT CAA GAT CCA AAT GCT ATG ACT GAT TGC TCC GAT GTT ATT CCA CAA TCT AAA CCT ATC CCA GGT AAC TTG CCA TTT TCA 
TTT TTT CCA GCT GGT AAG ACC ATC AAG GAT GTT GAA CAA GCT TGT GCT GAA ACT CCA TTT CCA ACT TTG ACT ACT TTG CCA GGT CCA GAA ACT TCT GTT 
CAA AGA ATA CCA CCA CCA CCA GGT GCT

[Improvement] BBa_K729006 / CueO Part-only sequence (1612bp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.

TTG ACA GCT AGC TCA GTC CTA GGT ATA ATG CTA GCT ACT AGA GAA AGA GGA GAA ATA CTA GAT GCA ACG TCG TGA TTT CTT AAA ATA TTC CGT CGC GCT 
GGG TGT GGC TTC GGC TTT GCC GCT GTG GAG CCG CGC AGT ATT TGC GGC AGA ACG CCC AAC GTT ACC GAT CCC TGA TTT GCT CAC GAC CGA TGC CCG TAA 
TCG CAT TCA GTT AAC TAT TGG CGC AGG CCA GTC CAC CTT TGG CGG GAA AAC TGC AAC TAC CTG GGG CTA TAA CGG CAA TCT GCT GGG GCC GGC GGT GAA 
ATT ACA GCG CGG CAA AGC GGT AAC GGT TGA TAT CTA CAA CCA ACT GAC GGA AGA GAC AAC GTT GCA CTG GCA CGG GCT GGA AGT ACC GGG TGA AGT CGA 
CGG CGG CCC GCA GGG AAT TAT TCC GCC AGG TGG CAA GCG CTC GGT GAC GTT GAA CGT TGA TCA ACC TGC CGC TAC CTG CTG GTT CCA TCC GCA TCA GCA 
CGG CAA AAC CGG GCG ACA GGT GGC GAT GGG GCT GGC TGG GCT GGT GGT GAT TGA AGA TGA CGA GAT CCT GAA ATT AAT GCT GCC AAA ACA GTG GGG TAT 
CGA TGA TGT TCC GGT GAT CGT TCA GGA TAA GAA ATT TAG CGC CGA CGG GCA GAT TGA TTA TCA ACT GGA TGT GAT GAC CGC CGC CGT GGG CTG GTT TGG 
CGA TAC GTT GCT GAC CAA CGG TGC AAT CTA CCC GCA ACA CGC TGC CCC GCG TGG TTG GCT GCG CCT GCG TTT GCT CAA TGG CTG TAA TGC CCG TTC GCT 
CAA TTT CGC CAC CAG CGA CAA TCG CCC GCT GTA TGT GAT TGC CAG CGA CGG TGG TCT GCT ACC TGA ACC AGT GAA GGT GAG CGA ACT GCC GGT GCT GAT 
GGG CGA GCG TTT TGA AGT GCT GGT GGA GGT TAA CGA TAA CAA ACC CTT TGA CCT GGT GAC GCT GCC GGT CAG CCA GAT GGG GAT GGC GAT TGC GCC GTT 
TGA TAA GCC TCA TCC GGT AAT GCG GAT TCA GCC GAT TGC TAT TAG TGC CTC CGG TGC TTT GCC AGA CAC ATT AAG TAG CCT GCC TGC GTT ACC TTC GCT 
GGA AGG GCT GAC GGT ACG CAA GCT GCA ACT CTC TAT GGA CCC GAT GCT CGA TAT GAT GGG GAT GCA GAT GCT AAT GGA GAA ATA TGG CGA TCA GGC GAT 
GGC CGG GAT GGA TCA CAG CCA GAT GAT GGG CCA TAT GGG GCA CGG CAA TAT GAA TCA TAT GAA CCA CGG CGG GAA GTT CGA TTT CCA CCA TGC CAA CAA 
AAT CAA CGG TCA GGC GTT TGA TAT GAA CAA GCC GAT GTT TGC GGC GGC GAA AGG GCA ATA CGA ACG TTG GGT TAT CTC TGG CGT GGG CGA CAT GAT GCT 
GCA TCC GTT CCA TAT CCA CGG CAC GCA GTT CCG TAT CTT GTC AGA AAA TGG CAA ACC GCC AGC GGC TCA TCG CGC GGG CTG GAA AGA TAC CGT TAA GGT 
AGA AGG TAA TGT CAG CGA AGT GCT GGT GAA GTT TAA TCA CGA TGC ACC GAA AGA ACA TGC TTA TAT GGC GCA CTG CCA TCT GCT GGA GCA TGA AGA TAC 
GGG GAT GAT GTT AGG GTT TAC GGT ATA A

[New] BBa_K1336005 / ispB Part-only sequence (972bp) [Link, see EG]

The first sequence below shows the "sense" ispB gene, but we use the second sequence, the Reverse Complement to design primers to produce an antisense fragment for gene knockdown (i.e. as RNAi).

"Sense" ispB sequence

ATG AAT TTA GAA AAA ATC AAT GAG TTA ACC GCG CAA GAT ATG GCG GGT GTT AAT GCG GCA ATC CTT GAG CAG CTT AAT TCC GAC GTC CAA CTG ATC AAT 
CAG TTA GGC TAT TAC ATC GTC AGC GGC GGC GGT AAA CGT ATT CGT CCG ATG ATT GCT GTA CTG GCT GCA CGA GCT GTT GGC TAT GAG GGA AAT GCG CAT 
GTC ACC ATT GCT GCC CTG ATC GAG TTT ATC CAC ACG GCG ACT CTG CTA CAC GAC GAC GTT GTG GAT GAA TCA GAT ATG CGC AGG GGT AAA GCT ACC GCC 
AAC GCC GCA TTT GGC AAT GCC GCC AGC GTG CTG GTA GGC GAT TTT ATT TAT ACC CGC GCT TTC CAG ATG ATG ACC AGC CTC GGT TCG CTC AAA GTG CTG 
GAA GTC ATG TCA GAA GCC GTA AAC GTC ATC GCA GAA GGT GAA GTT CTG CAA CTG ATG AAC GTT AAC GAT CCG GAC ATC ACT GAA GAA AAC TAC ATG CGC 
GTT ATC TAT AGC AAA ACC GCG CGT CTG TTT GAG GCT GCC GCG CAG TGT TCC GGG ATT CTG GCT GGC TGT ACG CCG GAG GAG GAG AAA GGC CTG CAG GAT 
TAT GGG CGC TAT CTC GGC ACT GCT TTC CAG TTG ATC GAC GAT TTA CTC GAT TAC AAT GCC GAT GGC GAA CAG TTA GGT AAA AAT GTC GGC GAC GAT CTG 
AAC GAA GGT AAA CCG ACG CTG CCG CTG CTG CAT GCG ATG CAT CAT GGC ACA CCA GAA CAG GCA CAG ATG ATC CGT ACC GCC ATC GAA CAG GGT AAC GGT 
CGC CAT CTT CTG GAA CCG GTT CTG GAA GCA ATG AAC GCT TGT GGA TCT CTT GAA TGG ACG CGT CAG CGT GCC GAG GAA GAA GCA GAC AAA GCC ATC GCA 
GCG TTA CAG GTG CTC CCG GAC ACC CCT TGG CGA GAA GCA CTC ATC GGC CTC GCG CAC ATC GCT GTT CAA CGC GAT CGT TAA

"Antisense" ispB sequence

TTA ACG ATC GCG TTG AAC AGC GAT GTG CGC GAG GCC GAT GAG TGC TTC TCG CCA AGG GGT GTC CGG GAG CAC CTG TAA CGC TGC GAT GGC TTT GTC TGC 
TTC TTC CTC GGC ACG CTG ACG CGT CCA TTC AAG AGA TCC ACA AGC GTT CAT TGC TTC CAG AAC CGG TTC CAG AAG ATG GCG ACC GTT ACC CTG TTC GAT 
GGC GGT ACG GAT CAT CTG TGC CTG TTC TGG TGT GCC ATG ATG CAT CGC ATG CAG CAG CGG CAG CGT CGG TTT ACC TTC GTT CAG ATC GTC GCC GAC ATT 
TTT ACC TAA CTG TTC GCC ATC GGC ATT GTA ATC GAG TAA ATC GTC GAT CAA CTG GAA AGC AGT GCC GAG ATA GCG CCC ATA ATC CTG CAG GCC TTT CTC 
CTC CTC CGG CGT ACA GCC AGC CAG AAT CCC GGA ACA CTG CGC GGC AGC CTC AAA CAG ACG CGC GGT TTT GCT ATA GAT AAC GCG CAT GTA GTT TTC TTC 
AGT GAT GTC CGG ATC GTT AAC GTT CAT CAG TTG CAG AAC TTC ACC TTC TGC GAT GAC GTT TAC GGC TTC TGA CAT GAC TTC CAG CAC TTT GAG CGA ACC 
GAG GCT GGT CAT CAT CTG GAA AGC GCG GGT ATA AAT AAA ATC GCC TAC CAG CAC GCT GGC GGC ATT GCC AAA TGC GGC GTT GGC GGT AGC TTT ACC CCT 
GCG CAT ATC TGA TTC ATC CAC AAC GTC GTC GTG TAG CAG AGT CGC CGT GTG GAT AAA CTC GAT CAG GGC AGC AAT GGT GAC ATG CGC ATT TCC CTC ATA 
GCC AAC AGC TCG TGC AGC CAG TAC AGC AAT CAT CGG ACG AAT ACG TTT ACC GCC GCC GCT GAC GAT GTA ATA GCC TAA CTG ATT GAT CAG TTG GAC GTC 
GGA ATT AAG CTG CTC AAG GAT TGC CGC ATT AAC ACC CGC CAT ATC TTG CGC GGT TAA CTC ATT GAT TTT TTC TAA ATT CAT

[New] BBa_K1336006 / LacI Expression Cassette + ispB Part-only sequence (972bp) [Link, see EG]

LacI EC + K1336005.

LacI EC + "Antisense" ispB Device sequence

LACIEC NEEDED + PREFIX NEEDED + TTA ACG ATC GCG TTG AAC AGC GAT GTG CGC GAG GCC GAT GAG TGC TTC TCG CCA AGG GGT GTC CGG GAG CAC CTG TAA CGC TGC GAT GGC TTT GTC TGC 
TTC TTC CTC GGC ACG CTG ACG CGT CCA TTC AAG AGA TCC ACA AGC GTT CAT TGC TTC CAG AAC CGG TTC CAG AAG ATG GCG ACC GTT ACC CTG TTC GAT 
GGC GGT ACG GAT CAT CTG TGC CTG TTC TGG TGT GCC ATG ATG CAT CGC ATG CAG CAG CGG CAG CGT CGG TTT ACC TTC GTT CAG ATC GTC GCC GAC ATT 
TTT ACC TAA CTG TTC GCC ATC GGC ATT GTA ATC GAG TAA ATC GTC GAT CAA CTG GAA AGC AGT GCC GAG ATA GCG CCC ATA ATC CTG CAG GCC TTT CTC 
CTC CTC CGG CGT ACA GCC AGC CAG AAT CCC GGA ACA CTG CGC GGC AGC CTC AAA CAG ACG CGC GGT TTT GCT ATA GAT AAC GCG CAT GTA GTT TTC TTC 
AGT GAT GTC CGG ATC GTT AAC GTT CAT CAG TTG CAG AAC TTC ACC TTC TGC GAT GAC GTT TAC GGC TTC TGA CAT GAC TTC CAG CAC TTT GAG CGA ACC 
GAG GCT GGT CAT CAT CTG GAA AGC GCG GGT ATA AAT AAA ATC GCC TAC CAG CAC GCT GGC GGC ATT GCC AAA TGC GGC GTT GGC GGT AGC TTT ACC CCT 
GCG CAT ATC TGA TTC ATC CAC AAC GTC GTC GTG TAG CAG AGT CGC CGT GTG GAT AAA CTC GAT CAG GGC AGC AAT GGT GAC ATG CGC ATT TCC CTC ATA 
GCC AAC AGC TCG TGC AGC CAG TAC AGC AAT CAT CGG ACG AAT ACG TTT ACC GCC GCC GCT GAC GAT GTA ATA GCC TAA CTG ATT GAT CAG TTG GAC GTC 
GGA ATT AAG CTG CTC AAG GAT TGC CGC ATT AAC ACC CGC CAT ATC TTG CGC GGT TAA CTC ATT GAT TTT TTC TAA ATT CAT + SUFFIX NEEDED

[New] BBa_K1336007 / LacI Expression Cassette + BsDyP Part-only sequence (XXXbp) [Link, see EG]

LacI EC + K1336003.

LacI EC + BsDyP Device sequence

1 100 199 298 397 496 595 694 793 892	LACIEC NEEDED + PREFIX NEEDED + [[BsDyP NEEDED]] + SUFFIX NEEDED

Plasmid Backbone / Vectors

pET-21a (+) sequence (Xbp) [Link, see DT]

...

atccggatatagttcctcctttcagcaaaaaacccctcaagacccgtttagaggccccaaggggttatgctagttattgc
tcagcggtggcagcagccaactcagcttcctttcgggctttgttagcagccggatctcagtggtggtggtggtggtgctc
gagtgcggccgcaagcttgtcgacggagctcgaattcggatccgcgacccatttgctgtccaccagtcatgctagccata
tgtatatctccttcttaaagttaaacaaaattatttctagaggggaattgttatccgctcacaattcccctatagtgagt
cgtattaatttcgcgggatcgagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcg
gttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcgg
cgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcgg
tgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacac
catcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaac
cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcg
gcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt
ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctccc
atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccatta
agttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgc
tggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg
gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct
ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactcattaggcaccgg
gatctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca
cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctt
tcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactg
gtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccccacgggtgcgcatgatcgtgctc
ctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtg
aagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctgg
aaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacct
acatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgttt
accctcacaacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggta
tcattacccccatgaacagaaatcccccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcc
cgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtga
atcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacaca
tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggt
gttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatca
gagcagattgtactgagagtgcaccatatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcagg
cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc
ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc
gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcag
aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac
cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc
tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc
ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag
agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta
tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc
ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg
gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc
ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatc
agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat
acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa
taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc
cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacg
ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaa
aagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagca
ctgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaga
atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaag
tgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgc
cgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatc
agggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccc
cgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttt
taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagttt
ggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccacta
cgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccg
atttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgc
tggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcg
cca

pSB1C3 Part-only sequence (2070bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaatt cattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatc cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagtt ggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca gaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1A3 Part-only sequence (2155bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtca ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttc ctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaa aaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1K3 Part-only sequence (2204bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaa aaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactca ccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccatt acgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatta caaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttt tcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtct gaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgca cctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaa tatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagatt ttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatga cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttct agag

BioBricks from iGEM Parts Registry Distributions

[Test use] J04450 Part-only sequence (1069bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcg tggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgc ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

[Used] K314103 Part-only sequence (1638bp)

acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgcttt cttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgac cccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtg gactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatga gctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcg cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgt tccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgca ttgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgatacc gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggc cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggat aacaagatactgagcacatactagagaaagaggagaaa

[Test use] R0010 Part-only sequence (200bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca

[Not used] R0011 Part-only sequence (55bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

[Not used] K206000 Part-only sequence (130bp)

acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctac tgtttctccataccgtttttttgggctagc

[Test use] B0034 Part-only sequence (12bp)

aaagaggagaaa

[Test use] K518012 Part-only sequence (828bp)

tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa cgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca gattattaatccggcttttttattattt

[Not used] B0012 Part-only sequence (41bp)

tcacactggctcaccttcgggtgggcctttctgcgtttata

Full table

No.	ID	Name / Function	Source	State / Concentration / Date Made	Gene Size / Sequence	Initial Plasmid / Vector	Comments
1	pAzoR / BBa_K1336000	FMN-dependent NADH-azoreductase 1	Pseudomonas putida	Miniprep, 48 ng/uL, 15/07/17, TO.	612 bp	Expression vector pET-21a (+) (ampicillin resistant (ampR)), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
2	p1B6 (AzoR 1B6) / BBa_K1336001	Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1	Pseudomonas putida	Miniprep, 68 ng/uL, 15/07/14, TO.	612 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
3	pCotA / BBa_K1336002	Spore Coat Protein Laccase	Bacillus subtilis	Miniprep, 103 ng/uL, 15/07/14, TO.	1542 bp	Expression vector pET-21a (+) (ampR), initially cloned between *NheI* and BamHI.	Plasmid provided by Lisbon; with Paper.
4	pBsDyP / BBa_K1336003	Dye Decolourising Peroxidase BSU38260	Bacillus subtilis	Miniprep, 51 ng/uL, 15/07/14, TO.	1251 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
5	pPpDyP / BBa_K1336004	Dye Decolourising Peroxidase PP_3248	Pseudomonas putida	Miniprep, 55 ng/uL, 15/07/14, TO.	864 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
6	LiP / BBa_K500000	Lignin Peroxidase	Phanerochaete chrysosporium (White-Rot Fungi)	[Taken from BBa_K500000, and optimised for use in E. coli; being synthesised by Gene Oracle], X ng/uL, dd/mm/yy, Gene Oracle.	1116 bp	[To be cloned directly into expression vector, pSB1C3, between EcoRI and PstI.]	Synthesised (for free) by Gene Oracle. Sequence from Paper.
7	CueO / BBa_K729006	Laccase	Escherichia coli	Miniprep, X ng/uL, dd/mm/yy, TO.	1612 bp	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).	A device consisting of: a constituitive promoter (J23119), a strong RBS (B0034), and laccase for polyethylene degradation (K729002).
8	ispB / BBa_K1336005	Octaprenyl Diphosphate Synthase	Escherichia coli, strain K-12	Miniprep, X ng/uL, dd/mm/yy, EG.	972 bp	[To be cloned directly into expression vector, pSB1C3, between EcoRI and PstI.]	Using the antisense/reverse complement gene as a method of gene knockdown (i.e. as RNAi); in collaboration with the Edinburgh iGEM 2014 Team.
9	BBa_J04450	RFP Coding Device	Spring 2014 BioBrick Distribution. Plate 4, Well 4B. [Check! DT?].	(1) Miniprep, 333 ng/uL, 01/07/14, TO. (2) Miniprep, 38 ng/uL (NanoDrop, dodgy!) 01/07/14, TO.	1069 bp, Genomic Sequence[Add sequence! Made from combined BioBricks?]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).	LacI-, and CAP-, sensitive; can fail if system contains LacI or CAP protein! RFP Coding Device contains: LacI (R0010), strong RBS (B0034), mRFP1 (E1010), and double terminator (B0015 = B0010+B0012).
10	BBa_K314103	Lac induced expression cassette	Spring 2014 BioBrick Distribution. Plate 1, Well 4D. [Check! DT?].	Miniprep, 334 ng/uL, 01/07/14, TO.	1638 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Lactose (IPTG) inducible protein expression insert includes f1 origin (K314110), a Lac I generator (K314111), a lactose inducible promoter (R0011), and the Elowitz standard RBS (B0034).
11	BBa_R0010	Promoter (LacI regulated)	Spring 2014 BioBrick Distribution. Plate 3, Well 4G. [Check! DT?].	Miniprep, 329.1 ng/uL, 01/07/14, TO.	200 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	This part is an inverting regulator sensitive to LacI and CAP. In the absence of LacI and CAP proteins, this part promotes transcription; in their presence, the part inhibits transcription. LacI can be inhibited by IPTG.
12	BBa_R0011	Promoter (LacI regulated, lambda pL hybrid)	Spring 2014 BioBrick Distribution. Plate 2, Well 6D (Inconsistent sequencing!). [Check! DT? / Maybe use Spring 2013 Distribution, Plate 5, Well 6G.].	Miniprep, 38 ng/uL (NanoDrop, dodgy!), 01/07/14, TO.	55 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.
13	BBa_K206000	pBAD Strong Promoter	Spring 2014 BioBrick Distribution. Plate 3, Well 14A. [Check! DT?].	Miniprep, 144 ng/uL, 01/07/14, TO.	130 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	pBAD is an E. coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription; in its presence, transcription is permitted.
14	BBa_B0034	RBS	Spring 2014 BioBrick Distribution. Plate 4, Well 1N. [Check! DT?].	Miniprep, 156.5 ng/uL, 01/07/14, TO.	12 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1A2, i.e. ampicillin resistant (ampR). Plasmid / Vector Map	RBS based on Elowitz (1999) repressilator.
15	BBa_K518012	RBS + RFP + double Terminator	Spring 2014 BioBrick Distribution. Plate 1, Well 18C. [Check! DT?].	(1) Miniprep, 49 ng/uL, 01/07/14, TO. (2) Miniprep, 219.2 ng/uL, 08/08/14, YKH.	828 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	This Coding Device contains: RBS.3 (medium) (B0032), mRFP1 (E1010), and double terminator (B0014 = B0012+B0011).
16	[CHECK: BAD PART !] BBa_B0012 (2)	Transcription Terminator for E. coli RNA polymerase	Spring 2014 BioBrick Distribution. Plate 2, Well 2B. [Check! DT?].	Miniprep, 128 ng/uL, 01/07/14, TO.	41 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	TE from coliphage T7. This is a bad terminator (Experience: Fails). It is a promoter in the reverse direction.
#	ID	Name / Function	Source	State / Concentration / Date Made	Gene Size / Sequence	Initial Plasmid / Vector	Comments