Team:UCL/Science/Sequences

Summary

For ease of reference, the sequences for (1) our BioBrick Gene Designs, (2) Plasmid / Vector Backbones, and (3) BioBricks that we have used from that iGEM Registry Distribution, have been provided below. Clicking on the Gene/Plasmid/BioBrick headers will take you to pages describing the part in more detail and the source of the sequences.

BioBrick Designs for Our Genes

[New] AzoR Part-only sequence (597/612bp) [Link, see DT]

Sequence taken from Lisbon.
ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGTCAGCTGAGCCGTGAAG TGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTTGAAGTGGTTTATCGCGACCTGGCTGCCGATGCCAT CGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCCCAGGCT TTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGCCGTGGTGATTGGTGCGC CCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGAC CTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCG GGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCA TCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGC GATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA

[New] AzoR 1B6 (heat-stable AzoR mutant) Part-only sequence (597/612bp) [Link, see DT]

Sequence given from Lisbon group directly.
ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGCCAGCTGAGCC GTGAAGTGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTGGAAGTGGTTTATCGCGACCTGGCTGCCGA TCCCATCGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCC CAGGCTTTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGGCGTGGTGATTG GTGCGCCCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGG CAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGAGCGGCGACAAGAAAGTGGTGCTGGTTTCC ACTGCGGGTGGCCAGCATGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGCGTTCCTGG GCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGACTATGGCCCGGAGCAGCGCAG CCAGGCGATAGATGCTGCACGGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA

[Proposed] CotA Part-only sequence (1733/1539bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgacacttgaaaaatttgtggatgctctcccaatcccagatacactaaa gccagtacagcaatcaaaagaaaaaacatactacgaagtcaccatggagg aatgcactcatcagctccatcgcgatctccctccaacccgcctgtggggc tacaacggcttatttccgggaccgaccattgaggttaaaagaaatgaaaa cgtatatgtaaaatggatgaataaccttccttccacgcatttccttccga ttgatcacaccattcatcacagtgacagccagcatgaagagcccgaggta aagactgttgttcatttacacggcggcgtcacgccagatgatagtgacgg gtatccggaggcttggttttccaaagactttgaacaaacaggaccttatt tcaaaagagaggtttatcattatccaaaccagcagcgcggggctatattg tggtatcacgatcacgccatggcgctcaccaggctaaatgtctatgccgg acttgtcggtgcatatatcattcatgacccaaaggaaaaacgcttaaaac tgccttcagacgaatacgatgtgccgcttcttatcacagaccgcacgatc aatgaggatggttctttgttttatccgagcgcaccggaaaacccttctcc gtcactgcctaatccttcaatcgttccggctttttgcggagaaaccatac tcgtcaacgggaaggtatggccatacttggaagtcgagccaaggaaatac cgattccgtgtcatcaacgcctccaatacaagaacctataacctgtcact cgataatggcggagattttattcagattggttcagatggagggctcctgc cgcgatctgttaaactgaattctttcagccttgcgcctgctgaacgttac gatatcatcattgacttcacagcatatgaaggagaatcgatcattttggc aaacagcgcgggctgcggcggtgacgtcaatcctgaaacagatgcgaata tcatgcaattcagagtcacaaaaccattggcacaaaaagacgaaagcaga aagccgaagtacctcgcctcatacccttcggtacagcatgaaagaataca aaacatcagaacgttaaaactggcaggcacccaggacgaatacggcagac ccgtccttctgcttaataacaaacgctggcacgatcccgtcacagaaaca ccaaaagtcggcacaactgaaatatggtccattatcaacccgacacgcgg aacacatccgatccacctgcatctagtctccttccgtgtattagaccggc ggccgtttgatatcgcccgttatcaagaaagcggggaattgtcctatacc ggtccggctgtcccgccgccgccaagtgaaaagggctggaaagacaccat tcaagcgcatgcaggtgaagtcctgagaatcgcggcgacattcggtccgt acagcggacgatacgtatggcattgccatattctagagcatgaagactat gacatgatgagaccgatggatataactgatccccataaataa

[New] BsDyP Part-only sequence (1251bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgagcgatgaacagaaaaagccagaacaaattcacagacgggacattttaaaatgggga gcgatggcgggggcagccgttgcgatcggtgccagcggtctcggcggtctcgctccgctt gttcagactgcggctaagccatcgaaaaaggatgaaaaagaggaggagcagattgttccg ttttacggaaagcatcaggccggaatcacaactgcccatcagacgtatgtctattttgct gcactggatgtaacagcaaaagacaaaagcgacatcattacgttatttagaaactggaca agtctcacacagatgctgacgtctggcaagaaaatgtcagcggagcagagaaatcagtat ctgccgccgcaggatacaggtgagtctgctgatttatccccttccaacttaacggtcaca ttcggattcgggcctggcttttttgaaaaagacggaaaggaccgcttcggactgaaaagc aaaaaaccgaagcacctagccgctcttccagcgatgcccaatgacaacctggatgaaaag cagggaggcggggacatctgcattcaagtgtgtgcagacgacgagcaagtggcgtttcac gcgctgcgtaacctgctaaatcaagcggtcggcacttgtgaggtccgttttgtgaacaaa ggctttttaagcggtggaaaaaatggtgaaacaccgcgcaacctcttcgggtttaaagat ggaacaggcaaccagagcacgaaggatgacacgttgatgaactcgatcgtgtggattcag tctggtgagcctgactggatgacgggcggcacctatatggcttttcggaaaatcaaaatg ttccttgaggtatgggaccgctcttccctcaaggaccaagaggataccttcggccgcaga aaaagctcgggggcgccttttgggcagaaaaaggaaacagatcccgtgaagctgaatcaa attccgtctaattcacacgtcagtctagcgaaatctactgggaaacaaattttgcgaaga gctttctcttacacagaaggacttgatccgaaaaccggctatatggatgcaggtctcctg tttatcagttttcaaaaaaatcccgacaatcagtttatcccgatgctgaaggctctttca gcaaaggatgcgttaaacgaatatacacaaacaatcggttctgctttatacgcatgccca ggcggctgcaaaaaaggagaatatattgcccagcgtttgctggaatcataa

[Proposed] PpDyP Part-only sequence (861/864bp) [Link, see DT]

Sequence taken from Lisbon.
ATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGC AGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCGCAGGTCGATGGCAAACAGTTGCTGCT GGGCGTAGGCGCCCCCTTGGCCAAGGCCCTGGGCCGGGAAATACCAGGCCTGCGCCCGTTCCCGCTACTG GATGCCGCGGTTGAGAACCCCAGCACCCAACACGCCCTGTGGCTGTGGCTGCGTGGCGACGAACGTGGCG ACCTGCTGCTGCGCACCCAGGCCCTGGAGCAGGCGCTGGCACCCGCCCTGAGCCTGGCCGACAGCGTTGA TGGCTTCCTGCACCGGGGCGGCCATGACCTTACCGGTTATGAAGACGGCACCGAAAACCCGACAGACGAA GAGGCGGTGCAAGCGGCCATTGCCGCAGATGGCTCCAGCTTTGCCGCGTTCCAGCTGTGGAAGCACGACC TGCAATACTTCAAGTCGCTGCCCCAGGCAGACCAGGACAACATCATCGGCCGTCGCTTGAGCGATAACGA AGAACTCGACGACGCCCCCGCGTCCGCGCACGTCAAGCGCACCGCCCAGGAAAGCTTTGAACCCGAGGCG TTCATGGTCCGTCGCTCGGTATCCTGGGCCGACCAACGCGGCGCCGGCCTGGCTTTCGTCGCCCTGGGCA AGAGTTTCGAGGCATTCGAGGTGCAGCTGCGGCGCATGAGTGGCCTGGAAGACGGCATCATCGACGGGCT GTACCGCTTCAGCCGCCCGCTGACGGGGGGCTACTACTGGTGCCCGCCAATGAGCGAGACGGGTGTCGAT CTAAGCCCCTTGCTGCGGGCCTGA

[Improvement] LiP Part-only sequence (1116bp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
atggcttttaaacaattattcgccgctatttccttggctttgtctttgtctgctgctaatgctgctgctgttattgaaaaaagagctacttgttccaacg gtaagactgttggtgatgcttgttcttgtgcttggtttgatgttttggacgacatccaacaaaacttgtttcatggtggtcaatgtggtgctgaagctca tgaatctatcagattggttttccatgactccattgctatttctccagctatggaagctcaaggtaaatttggtggaggtggtgctgatggttccattatg attttcgatgatatcgaaaccgccttccatccaaatattggtttggacgaaatcgtcaagttgcaaaagccatttgttcaaaagcacggtgttactccag gtgattttattgcttttgctggtgctgttgctttgtctaattgtccaggtgctccacaaatgaatttctttactggtagagcaccagctactcaaccagc tccagatggtttggttccagaaccatttcataccgttgaccaaatcatcaacagagttaatgacgccggtgaatttgacgaattggaattggtttggatg ttgtctgctcattctgttgctgctgttaatgatgttgatccaactgttcaaggtttgccatttgattctaccccaggtattttcgattcccaattcttcg ttgaaacccaattgagaggtactgcttttccaggttctggtggtaatcaaggtgaagttgaatctccattgccaggtgaaattagaatccaatccgatca taccattgccagagattcaagaactgcttgtgaatggcaatccttcgttaacaatcaatccaagttggttgacgacttccaattcattttcttggctttg actcaattgggtcaagatccaaatgctatgactgattgctccgatgttattccacaatctaaacctatcccaggtaacttgccattttcattttttccag ctggtaagaccatcaaggatgttgaacaagcttgtgctgaaactccatttccaactttgactactttgccaggtccagaaacttctgttcaaagaatacc accaccaccaggtgct

[Improvement] Laccase Part-only sequence (Xbp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcaacgtcgtgatttcttaaaatattccgtcgcgctg ggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatc gcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaatt acagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggc ggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggca aaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatga tgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacg ttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcg ccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcg ttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcct catccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctga cggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatgga tcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcag gcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgacatgatgctgcatccgttccata tccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcag cgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggatgatgttaggg tttacggtataa

[New] ispB Part-only sequence (972bp) [Link, see EG]

Sequence shows the ispB gene, but we use the Reverse Complement to design primers to produce an antisense fragment for gene knockdown (i.e. as RNAi).ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGT TAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATC AGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATG ATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGCATGT CACCATTGCTGCCCTGATCGAGTTTATCCACACGGCGACTCTGCTACACG ACGACGTTGTGGATGAATCAGATATGCGCAGGGGTAAAGCTACCGCCAAC GCCGCATTTGGCAATGCCGCCAGCGTGCTGGTAGGCGATTTTATTTATAC CCGCGCTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAG TCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTG ATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTAT CTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGA TTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGG CGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAA TGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAG GTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAA CAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCT TCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGA CGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAG GTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACAT CGCTGTTCAACGCGATCGTTAA

[New] ispB Reverse Complement Part-only sequence (972bp) [Link, see DT]

TTAACGATCGCGTTGAACAGCGATGTGCGCGAGGCCGATGAGTGCTTCTC GCCAAGGGGTGTCCGGGAGCACCTGTAACGCTGCGATGGCTTTGTCTGCT TCTTCCTCGGCACGCTGACGCGTCCATTCAAGAGATCCACAAGCGTTCAT TGCTTCCAGAACCGGTTCCAGAAGATGGCGACCGTTACCCTGTTCGATGG CGGTACGGATCATCTGTGCCTGTTCTGGTGTGCCATGATGCATCGCATGC AGCAGCGGCAGCGTCGGTTTACCTTCGTTCAGATCGTCGCCGACATTTTT ACCTAACTGTTCGCCATCGGCATTGTAATCGAGTAAATCGTCGATCAACT GGAAAGCAGTGCCGAGATAGCGCCCATAATCCTGCAGGCCTTTCTCCTCC TCCGGCGTACAGCCAGCCAGAATCCCGGAACACTGCGCGGCAGCCTCAAA CAGACGCGCGGTTTTGCTATAGATAACGCGCATGTAGTTTTCTTCAGTGA TGTCCGGATCGTTAACGTTCATCAGTTGCAGAACTTCACCTTCTGCGATG ACGTTTACGGCTTCTGACATGACTTCCAGCACTTTGAGCGAACCGAGGCT GGTCATCATCTGGAAAGCGCGGGTATAAATAAAATCGCCTACCAGCACGC TGGCGGCATTGCCAAATGCGGCGTTGGCGGTAGCTTTACCCCTGCGCATA TCTGATTCATCCACAACGTCGTCGTGTAGCAGAGTCGCCGTGTGGATAAA CTCGATCAGGGCAGCAATGGTGACATGCGCATTTCCCTCATAGCCAACAG CTCGTGCAGCCAGTACAGCAATCATCGGACGAATACGTTTACCGCCGCCG CTGACGATGTAATAGCCTAACTGATTGATCAGTTGGACGTCGGAATTAAG CTGCTCAAGGATTGCCGCATTAACACCCGCCATATCTTGCGCGGTTAACT CATTGATTTTTTCTAAATTCAT

Plasmid Backbone / Vectors

pET-21a (+) sequence (Xbp) [Link, see DT]

atccggatatagttcctcctttcagcaaaaaacccctcaagacccgtttagaggccccaaggggttatgctagttattgc tcagcggtggcagcagccaactcagcttcctttcgggctttgttagcagccggatctcagtggtggtggtggtggtgctc gagtgcggccgcaagcttgtcgacggagctcgaattcggatccgcgacccatttgctgtccaccagtcatgctagccata tgtatatctccttcttaaagttaaacaaaattatttctagaggggaattgttatccgctcacaattcccctatagtgagt cgtattaatttcgcgggatcgagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcg gttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcgg cgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcgg tgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacac catcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaac cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcg gcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctccc atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccatta agttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgc tggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactcattaggcaccgg gatctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctt tcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactg gtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccccacgggtgcgcatgatcgtgctc ctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtg aagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctgg aaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacct acatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgttt accctcacaacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggta tcattacccccatgaacagaaatcccccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcc cgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtga atcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacaca tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggt gttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatca gagcagattgtactgagagtgcaccatatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcagg cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcag aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatc agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacg ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaa aagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagca ctgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaga atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaag tgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgc cgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatc agggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccc cgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttt taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagttt ggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccacta cgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccg atttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgc tggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcg cca

pSB1C3 Part-only sequence (2070bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaatt cattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatc cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagtt ggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca gaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1A3 Part-only sequence (2155bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtca ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttc ctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaa aaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1K3 Part-only sequence (2204bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaa aaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactca ccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccatt acgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatta caaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttt tcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtct gaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgca cctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaa tatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagatt ttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatga cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttct agag

BioBricks from iGEM Parts Registry Distributions

[Test use] J04450 Part-only sequence (1069bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcg tggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgc ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

[Used] K314103 Part-only sequence (1638bp)

acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgcttt cttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgac cccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtg gactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatga gctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcg cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgt tccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgca ttgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgatacc gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggc cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggat aacaagatactgagcacatactagagaaagaggagaaa

[Test use] R0010 Part-only sequence (200bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca

[Not used] R0011 Part-only sequence (55bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

[Not used] K206000 Part-only sequence (130bp)

acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctac tgtttctccataccgtttttttgggctagc

[Test use] B0034 Part-only sequence (12bp)

aaagaggagaaa

[Test use] K518012 Part-only sequence (828bp)

tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa cgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca gattattaatccggcttttttattattt

[Not used] B0012 Part-only sequence (41bp)

tcacactggctcaccttcgggtgggcctttctgcgtttata