Team:UCL/Humans/Attributions

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    <li><a href="#view1">Overview</a></li>
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<div class="textTitle"><h4>Acknowledgements & Attributions</h4></div>
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<div class="textTitle"><h3>Acknowledgements & Attributions</h3></div><br>
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<p>The UCL iGEM 2014 team would like to thank all our advisors who have assisted us throughout the project, and without whom the project would not have been possible. We would also like to thank all everyone else who has helped us realise this project, be it through invaluable advice or providing DNA, seeds, or other materials. These contributions have helped us enormously. All work on this wiki was carried out and all data collected by us unless stated otherwise. For a full list of our collaborations & acknowledgements, please visit our <a href="https://2014.igem.org/Team:UCL/Humans/Collab">collaborations</a> page.</p><br>
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<p>The UCL iGEM 2014 team would like to thank all our advisors who have assisted us throughout the project, and without whom the project would not have been possible. We would also like to thank all everyone else who has helped us realise this project, be it through invaluable advice or providing DNA, equipment, or other materials. These contributions have helped us enormously. All work on this wiki was carried out and all data collected by us unless stated otherwise. For a full list of our collaborations & acknowledgements, please visit our <a href="https://2014.igem.org/Team:UCL/Humans/Collab">collaborations</a> page.</p><br>
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<p>One of the major hurdles in microfluidics is the fact that fluids are very difficult to mix at small volumes as a result of low <a href="http://en.wikipedia.org/wiki/Reynolds_number">Reynolds numbers</a>. Reynolds number describes flow patterns in various flow situations; a low Reynolds number indicates laminar flow, whereas a large Reynolds number indicates turbulent flow. Turbulent flow allows highly effective mixing in comparison to laminar flow. However, in microfluidics several device designs are available, which (to a greater or lesser degree) allow sufficient mixing. The flip side of this is that diffusion is often the only method of mixing. This in itself can be very useful, particularly when investigating enzyme kinetics. </p><br>
 
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<p><img src="https://static.igem.org/mediawiki/2014/2/22/UCLDAVEJACKSONMF.jpg" align="right" width="20%">The scope and applications of microfluidics is truly immense and as time goes on, ever increasing amounts of investment is made. With more start-up companies and takeover bids in play, it is clear microfluidics is an exciting and emerging scientific field. As a powerful biological research tool, microfluidics allows the adaptation of various molecular biology techniques - ranging from on-chip gene synthesis (protein expression from coding DNA) to the screening of protein interactions. <br><br>Here's a picture of Dave Jackson "working hard" in the microfluidics lab.</p></div>
 
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<p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br>
 
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<p>Our work and achievements can be split into 6 categories, each representing the different aspects of our immense project. During the early days of our project, we identified the different sub-teams we would be working in, and since then a lot of members have contributed to almost all aspects of the project.<br><br></p>
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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<br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div>
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<h3>Lab: BioBricks</h3>
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<p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work.<br><br>
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<a href="http://www.itqb.unl.pt/martins">Microbial & Enzyme Technology Lab</a> led by Dr Lígia O. Martins at the Universidade Nova de Lisboa for providing us plasmid and advice for our work on azo dyes degradation
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<p>We will use rapid polymer prototyping techniques to generate microfluidic chips that will allow us to test our reaction and aid in the construction of a realistic bioprocess, which can be successfully scaled-up for industrial use. As we optimise and change our bioprocess, we can also quickly design new microfluidic chips that can mimic its development on a micro-scale. For example, it is our goal to integrate multiple downstream steps, such as chromatography, in order to isolate potential useful products. Demonstrating this in a microfluidic system is less time-consuming and far more cost effective than doing so at a larger scale.</p><br>
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<p>For our microfluidic bioreactor, we will be using a magnetic free floating bar as our mixing system. This is an effective method of mixing at a microfluidic scale, as demonstrated in the video on the right. This video is of a microfluidic chemostat bioreactor designed by Davies et al. 2014 UCL, using a free-floating bar to mix two dyes.</p><br>
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<h4><b>Team members:</b></h4> Georgia Bondy, Daniel de la Torre, Adam Denyer, Edoardo Gianni, Yan Kay Ho, Sohaila Jalali, Sanjay Joshi, Behzad Karkaria, Ning Lu, Tanel Ozdemir
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Above are some examples of the microfluidics devices developed by our team for use in the lab at the UCL ACBE. The devices are initially designed using AutoCAD (2D and 3D computer-aided design software), once the designs are finalised they can be 3D-printed using the facilities provided by the UCL Institute of Making and UCL ACBE; allowing our bioprocess and laboratory team to experiment and improve designs.</p></div>
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<h4><b>Contributions from:</b></h4> Darren Nesbeth, Vitor Pinhiero, Helina Marshall, Alex Templar, Des Schofield, Archie Melbourne, Julian Albers</p>
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An example of one of our microfluidic devices designed on AutoCAD can be downloaded <a href="https://static.igem.org/mediawiki/2014/f/fa/UCL_iGEM_2014_Microfluidics_Device_Design.dwg.zip">here</a>. This device utilises the basic concept of mixing the cells and dyes, producing a single output stream; much alike to the <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocessing</a> concept. During the course of designing the microfluidic device, several key considerations must be taken into account: ability to withstand high pressure without leakage; materials of construction to be inert and transparent; size constraints of inlet and outlet piping; ability to accurately 3D-print the device.</p><br>
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<p>We would like to thank Vista Print for helping us produce our business cards, and we are also grateful for the printing facilities provided by UCL and the UCL Biochemical Engineering department.
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<h4><b>Team members:</b></h4> Oran Maguire, Pamela Niem</p>
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<br><h4><b>Contributions from:</h4></b> Central St Martins: Cameo Bondy, Barbara Czepiel. The Slade: Jo Volley. Natsai Audrey, Linden Gledhill. </p>
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<p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br>
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<b><h4>Team members:</h4></b> Alberto Aparicio, Kevin Keyaert, Alex Bates, Georgia Bondy, Edoardo Gianni
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<br><h4><b>Contributions from:</h4></b> Julian Albers, Phillip Boeing, Andy Cheng, KaiCheng Kiew, Archie Melbourne, Bethan Wolfenden</p>
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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<br><br>The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.</p></div>
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<h3>Modelling & General Science</h3>
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<p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br>
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<p>We would like to thank the UCL Biochemical Engineering and the Mathematics and Physical Sciences department for providing us with the guidance, facilities and resources to complete our lab work.
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<b><h4>Team members:</h4></b> Miriam Leon, Rob Stanley, Georgia Bondy</p>
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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<p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br>
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<p>We would like to thank our 1000+ followers on Facebook, Twitter & Tumblr, as well as acknowledge the work of programmers whose open source work we utilised in the development of this Wiki. This wiki contains work sourced from Font Awesome and jQuery plugins, we are thankful to have been able to utilise these features. We extend our gratitude to the <a href="https://2011.igem.org/Team:DTU-Denmark/How_to_customize_an_iGEM_wiki">DTU-Denmark (2011)</a> and <a href="https://2010.igem.org/Team:TU_Munich/BeyondTheLab/WikiTutorial">TU Munich (2010)</a> teams for the tutorials they provided us from their respective wikis. Additional thanks to past UCL iGEM members, who helped us develop the design and concept for our Wiki.
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<b><h4>Team members:</h4></b> Sanjay Joshi, Adam Denyer
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<br><h4><b>Contributions from:</h4></b> Yan Kay Ho, Lewis Moffat</p>
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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<h3>Bioprocess Engineering</h3>
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<p>Since our project involves designing a novel <a href="https://2014.igem.org/Team:UCL/Science/Bioprocessing">bioprocess</a> using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.</p><br>
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<p>We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our work. Additional thanks go out to Professor Eli Keshavarz-Moore, Dr Frank Baganz, Professor Gary Lye, and Dr Yuhong Zhou; for the advice and guidance they provided us throughout our project. We have also <a href="https://2014.igem.org/Team:UCL/Humans/Collab">collaborated</a> with Godfrey Kyazze, a lecturer at University of Westminster. We would also like to acknowledge the respective authors of the research papers we studied.
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<b><h4>Team members:</h4></b>Maurice Bertrand, Sanjay Joshi, Joy Faucher</p>
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<h3>Microfluidics</h3>
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
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<b><h4>Team members:</h4></b> Lewis Brayshaw, David Jackson
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<b><h4>Contributions from:</h4></b> Joy Faucher, Sanjay Joshi, Edoardo Gianni</p>
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Latest revision as of 20:27, 15 April 2015

Goodbye Azodye UCL iGEM 2014

Attributions

Acknowledgements & Attributions


The UCL iGEM 2014 team would like to thank all our advisors who have assisted us throughout the project, and without whom the project would not have been possible. We would also like to thank all everyone else who has helped us realise this project, be it through invaluable advice or providing DNA, equipment, or other materials. These contributions have helped us enormously. All work on this wiki was carried out and all data collected by us unless stated otherwise. For a full list of our collaborations & acknowledgements, please visit our collaborations page.


Lab Graphics & Design Human Practices Modelling Web & Communication Bioprocessing

Our work and achievements can be split into 6 categories, each representing the different aspects of our immense project. During the early days of our project, we identified the different sub-teams we would be working in, and since then a lot of members have contributed to almost all aspects of the project.

Lab: BioBricks

We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work.

Microbial & Enzyme Technology Lab led by Dr Lígia O. Martins at the Universidade Nova de Lisboa for providing us plasmid and advice for our work on azo dyes degradation

Team members:

Georgia Bondy, Daniel de la Torre, Adam Denyer, Edoardo Gianni, Yan Kay Ho, Sohaila Jalali, Sanjay Joshi, Behzad Karkaria, Ning Lu, Tanel Ozdemir

Contributions from:

Darren Nesbeth, Vitor Pinhiero, Helina Marshall, Alex Templar, Des Schofield, Archie Melbourne, Julian Albers


Graphics & Design

We would like to thank Vista Print for helping us produce our business cards, and we are also grateful for the printing facilities provided by UCL and the UCL Biochemical Engineering department.

Team members:

Oran Maguire, Pamela Niem


Contributions from:

Central St Martins: Cameo Bondy, Barbara Czepiel. The Slade: Jo Volley. Natsai Audrey, Linden Gledhill.


Policy & Practices

We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work.

Team members:

Alberto Aparicio, Kevin Keyaert, Alex Bates, Georgia Bondy, Edoardo Gianni

Contributions from:

Julian Albers, Phillip Boeing, Andy Cheng, KaiCheng Kiew, Archie Melbourne, Bethan Wolfenden


Modelling & General Science

We would like to thank the UCL Biochemical Engineering and the Mathematics and Physical Sciences department for providing us with the guidance, facilities and resources to complete our lab work.

Team members:

Miriam Leon, Rob Stanley, Georgia Bondy


Web & Communications

We would like to thank our 1000+ followers on Facebook, Twitter & Tumblr, as well as acknowledge the work of programmers whose open source work we utilised in the development of this Wiki. This wiki contains work sourced from Font Awesome and jQuery plugins, we are thankful to have been able to utilise these features. We extend our gratitude to the DTU-Denmark (2011) and TU Munich (2010) teams for the tutorials they provided us from their respective wikis. Additional thanks to past UCL iGEM members, who helped us develop the design and concept for our Wiki.

Team members:

Sanjay Joshi, Adam Denyer

Contributions from:

Yan Kay Ho, Lewis Moffat


Bioprocess Engineering

We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our work. Additional thanks go out to Professor Eli Keshavarz-Moore, Dr Frank Baganz, Professor Gary Lye, and Dr Yuhong Zhou; for the advice and guidance they provided us throughout our project. We have also collaborated with Godfrey Kyazze, a lecturer at University of Westminster. We would also like to acknowledge the respective authors of the research papers we studied.

Team members:

Maurice Bertrand, Sanjay Joshi, Joy Faucher


Microfluidics

We would like to thank the UCL Biochemical Engineering department for providing us with the guidance, facilities and resources to complete our lab work.

Team members:

Lewis Brayshaw, David Jackson

Contributions from:

Joy Faucher, Sanjay Joshi, Edoardo Gianni

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

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