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Team:UCL/FAQ/MicroF
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Revision as of 21:27, 30 September 2014
What is Microfluidics?
Microfluidics is the study, design, and fabrication of devices that can handle and process fluids on the microlitre scale. Over the past few decades, this field has been responsible for many commonly used, and sometimes life-dependant, tools that people rely on. For example, glucose sensors for diabetics, is one of the plethora of handheld devices made available through microfluidics. The tiny volumes involved allow niche environments to be studied (e.g. in understanding stem cells), as well as permitting the development of high throughput screening devices (e.g. in drug discovery and development). 
One of the major hurdles in microfluidics is the fact that fluids are very difficult to mix at small volumes as a result of low Reynolds numbers. Reynolds number describes flow patterns in various flow situations; a low Reynolds number indicates laminar flow, whereas a large Reynolds number indicates turbulent flow. Turbulent flow allows highly effective mixing in comparison to laminar flow. However, in microfluidics several device designs are available, which (to a greater or lesser degree) allow sufficient mixing. The flip side of this is that diffusion is often the only method of mixing. This in itself can be very useful, particularly when investigating enzyme kinetics.
The scope and applications of microfluidics is truly immense and as time goes on, ever increasing amounts of investment is made. With more start-up companies and takeover bids in play, it is clear microfluidics is an exciting and emerging scientific field. As a powerful biological research tool, microfluidics allows the adaptation of various molecular biology techniques - ranging from on-chip gene synthesis (protein expression from coding DNA) to the screening of protein interactions.
Why Microfluidics?
Microfluidics allows us to manipulate fluids on a very small scale, as a result a miniaturised process contained within a microfluidic device provides us with several benefits. In our case, dealing with azo dyes in large quantities can be avoided as only small samples are required. Within the microfluidics lab, our team members are able to take advantage of the automation provided by microfluidics. As a result, less experienced team members can readily learn how to run the microfluidics experiments. Microfluidics ties in heavily with the progress of our bioprocessing team. As we explore different process designs, microfluidic devices can be easily designed and constructed for use and testing. Additionally, as our bioprocess develops and improves, microfluidics allows us to run the necessary tests and experiments without much demand for reagents and other consumables.
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The videos above were recorded in the UCL ACBE Microfluidics labs by members of our team. The video on the left is a demonstration of laminar flow across a T-junction microfluidic device. The video on the right demonstrates one of the methods of mixing made possible by microfluidics (herring bone channels etched into the chip).
The image on the right displays the microfluidics set-up used by our iGEM team. This device and equipment is provided for by the UCL microfluidics lab.
Since our project involves designing a novel bioprocess using whole-cell biocatalysts, microfluidics presents us with a unique and extremely useful advantage. When it comes to identifying, developing and optimising reactor designs and reaction constraints, this can be performed with ease and with low reagent cost as all variables are scaled down to a micro-level. Most importantly, the scale-down can be carried out without losing any of the accuracy or quantification of data output; this is due the number of sensors and control mechanisms which can be integrated into the microfluidic system.
We will use rapid polymer prototyping techniques to generate microfluidic chips that will allow us to test our reaction and aid in the construction of a realistic bioprocess, which can be successfully scaled-up for industrial use. As we optimise and change our bioprocess, we can also quickly design new microfluidic chips that can mimic its development on a micro-scale. For example, it is our goal to integrate multiple downstream steps, such as chromatography, in order to isolate potential useful products. Demonstrating this in a microfluidic system is far more time and cost effective than doing so at a larger scale.
Above are some examples of the microfluidics devices developed by our team for use in the Microfluidics Lab at the UCL ACBE. The devices are initially designed using AutoCAD (2D and 3D computer-aided design software), once the designs are finalised they can be 3D-printed using the facilities provided by the UCL Institute of Making; allowing our Bioprocess and Laboratory team to experiment and improve designs.
An example of one of our microfluidic devices designed on AutoCAD can be downloaded here. This device utilises the basic concept of mixing the cells and dyes, producing a single output stream; much alike to the bioprocessing concept. During the course of designing the microfluidic device, several key considerations must be taken into account: ability to withstand high pressure without leakage; materials of construction to be inert and transparent; size constraints of inlet and outlet piping; ability to accurately 3D-print the device.
