Team:TCU Taiwan/Safety
From 2014.igem.org
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<td colspan="3" height="1px" align="center"><strong><font face="Trebuchet MS" size="5" color="#A66B38">TCU_Taiwan iGEM Laboratory Practices</font></strong> | <td colspan="3" height="1px" align="center"><strong><font face="Trebuchet MS" size="5" color="#A66B38">TCU_Taiwan iGEM Laboratory Practices</font></strong> | ||
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<td width="30px" align="right"><font size="2" face="Verdana" color="#333">1.</font></td> | <td width="30px" align="right"><font size="2" face="Verdana" color="#333">1.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Don’t eat, drink, prepare or store food, smoke, handle contact lenses or apply cosmetics in any laboratory.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">2.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">2.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Know where the nearest eyewash, safety shower, and fire extinguisher are located. Know how to use them.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">3.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">3.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Insist upon good housekeeping in your laboratory.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">4.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">4.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Check for insects and rodents. Keep all areas clean.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">5.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">5.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Secure all gas cylinders.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333"><font size="2" face="Verdana" color="#333">6.</font></font></td> | <td align="right"><font size="2" face="Verdana" color="#333"><font size="2" face="Verdana" color="#333">6.</font></font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Wear laboratory coats and other appropriate protective clothing while performing laboratory activities. Feet and legs should be covered; sandals and open-toed shoes should not be worn in laboratories. Wear appropriate gloves while handling infectious or toxic materials and animals. Do not wear lab coats. Gloves or other personal protective equipment outside the laboratory.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">7.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">7.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Use a biological safety cabinet for handling infectious materials or materials requiring protection from contamination and a fume hood for toxic materials; mixed hazards need to be evaluated case by case.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">8.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">8.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Fume hoods should be used for laboratory activities that could result in chemical explosions or fires, for experiments involving toxic, hazardous or carcinogenic compounds, and use of strong acids and bases. Biological safety cabinets should not be used for this kind of work. Fume hoods are workstations, not storage cabinets. Vented storage areas may be located under the fume hood work area. However, these are not for storage of flammables.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">9.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">9.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Respect chemicals and radionuclides. Know their hazards and follow appropriate safety precautions. Chemical and radioactive waste must not be poured down the drain.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">10.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">10.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">All equipment must be documented to be free of chemical, biological, and radiological contamination before repair work is done or before moving equipment for storage or elsewhere.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">11.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">11.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Never mouth pipette anything. Use mechanical pipetting devices only!</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">12.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">12.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Close laboratory doors while experiments are in progress. Restrict access to laboratory.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">13.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">13.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Put liquid traps and in-line vacuum filters on all vacuum lines.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">14.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">14.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Minimize or contain all aerosol-producing activities, large-volume work, concentrated solutions or cultures. These activities include centrifugation (use safety cups), vortex missing (stopper tube), blending (use metal safety blender), sonication, grinding, opening containers of infectious material, inoculating culture flasks, inoculating animals, harvesting infectious materials from cultures or animals, and weighing or reconstructing toxic powders, etc.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">15.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">15.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Place biological safety cabinets in low-traffic areas and minimize activities that disrupt air flow in or around cabinet.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">16.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">16.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Decontaminate all work surfaces daily, and decontaminate all spills immediately.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">17.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">17.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Decontaminate (by autoclaving or chemical disinffection) all biologically contaminated materials – glassware, animal cages, laboratory equipment, etc. – before washing, reuse or disposal. Discard materials via proper waste stream.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">18.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">18.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Be careful with needles and syringes. Use only when alternative methods are not feasible.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">19.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">19.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Syringes, needles, Pasteur pipettes, etc, should be placed in rigid, leak-proof containers (Sharps Safe) and discarded following the waste rules.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">20.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">20.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Broken glassware and disposable pipettes (after decontamination) should be placed in a “Disposable Labware and Broken Glass Box” and discarded following the waste rules.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">21.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">21.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Place contaminated biological materials in covered, leak-proof containers before removing them from the laboratory.</font></td> |
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<td align="right"><font size="2" face="Verdana" color="#333">22.</font></td> | <td align="right"><font size="2" face="Verdana" color="#333">22.</font></td> | ||
- | <td><font size=" | + | <td><font size="3" face="Verdana" color="#333">Wash your hands after handling chemicals, infectious materials, animals, after removing gloves and before leaving the laboratory.</font></td> |
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</table> | </table> |
Revision as of 14:47, 6 October 2014
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TCU_Taiwan iGEM Laboratory Practices | ||||||||||||||||||||||||||||||||||||||||||||||
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Visit the Safety Hub to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project. Edit this page!Please use this page to write about anything related to safety in your project. Your LabUse this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! |
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