Team:Saarland/2 step

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<b>Bunk</b>, B., Schulz, A., Stammen, S., Munch, R., Warren, M.J., Rohde, M., Jahn, D., and Biedendieck, R. (2010). A short story about a big magic bug. Bioeng. Bugs 1, 85–91.<br>
<b>Bunk</b>, B., Schulz, A., Stammen, S., Munch, R., Warren, M.J., Rohde, M., Jahn, D., and Biedendieck, R. (2010). A short story about a big magic bug. Bioeng. Bugs 1, 85–91.<br>

Revision as of 11:52, 15 October 2014



2. Find allies and join forces


B. megaterium

Figure 1: Electron microscopy of B. megaterium. [http://megabac.tu-bs.de/graphics/bmeg.jpg]


B. megaterium belongs to the group of Gram-positive, aerobic bacteria. It’s typically found in soil, but can also survive in sea water or dried foods (Vary et al., 2007). Since its discovery in 1884, it has become one of the most important organisms for industrial and research purposes, because it combines numerous useful characteristics. Due to its extraordinary size of up to 1,5 µm x 4 µm and it`s impressive cell volume of more than 60 µm3, B. megateriumis predestined for studies regarding cell structure and protein localisation (Boyke et al., 2010; Vary et al., 1992). Furthermore it is able to metabolise more than 62 carbon sources, making its cultivation efficient and inexpensive (Millet et al., 1962). Probably the most useful characteristic for our purposes is its ability of preserving plasmids over multiple generations, making it a good specimen for the secretion of proteins and other organic molecules. Especially concerning the B. megaterium strain MS941 that was derived from the strain DSM319 by knockout of the neutral protease gene nprM (Wittchen et a l. 1995). For this reason the non sporulating strain MS941 is ideal for heterologous protein expression. This was shown by overexpression of GFP (Stammen et a l., 2007).

Additionally, the biosynthetic pathway of hyaluronic acid precursor molecules is already established in B. megaterium since UDP-N-acetyl-D-glucosamine and UDP-D-glucuronic acid are also essential components for cell wall synthesis in Gram-positive bacteria. Manual supplementation of these extremely expensive precursor molecules is not longer necessary. This could contribute to a future profitable biotechnological production of the high molecular mass hyaluronic acid (HMM-HA) of the naked mole rat. Homology searches in the B. megaterium genome have also shown that there are no endogenous hyaluronidases, which would otherwise immediately degrade the produced hyaluronic acid. Furthermore B. megaterium does not possess an endogenous hyaluronan synthase (Has). For this reason our team can be sure that hyaluronic acid has exclusively been synthesised by the correct enzyme and features the correct molecular weight and anti carcinogenic properties.

For the optimisation of hyaluronic acid production our team intends to overexpress endogenous B. megaterium proteins that are necessary for the production of hyaluronic acid precursor molecules. We think that the flow equilibrium of metabolites will be shifted towards production of HA, rather than to cell wall components. This approach could make a considerable contribution for high yield production of HMM-HA. The identification of corresponding genes in B. megaterium is based on the biosynthetic pathway for the HA production in group A and group C streptococci as well as for HA production in B. subtilis (Widner et al. 2005). The proposed biosynthetic pathway for production of the HA precursor molecules in B. megaterium after in silico gene homology search on MegaBac v9 platform is shown in figure 2.

Figure 2: Proposed biosynthetic pathway for HA production in B. megaterium.It is based on the corresponding pathway in group A and group C streptococci. Alterations have been made after conducting homology searches in B. megaterium genome on MegaBac v9 platform. The has2 gene originates from the naked mole rat with adapted codon usage for expression in B. megaterium.


However, when the HA will be successfully produced as we described in figure 2, then it is supposed to be secreted into the medium. Since studies with the similar B. subtilis expressing a streptococci hyaluronan synthase (HasA) have already shown similar results. It’s either possible that there is a specific transporter for oligosaccharides mediating HA secretion into the medium, or that the multi membrane domains of the hyaluronan synthase themselves form a channel for the secretion of the nascent HA chain (Weigel, 2002)

Modeling

Previous Step



References


Bunk, B., Schulz, A., Stammen, S., Munch, R., Warren, M.J., Rohde, M., Jahn, D., and Biedendieck, R. (2010). A short story about a big magic bug. Bioeng. Bugs 1, 85–91.

Schaeffer, P., Millet, J., and Aubert, J.P. (1965). Catabolic repression of bacterial sporulation. Proc. Natl. Acad. Sci. U. S. A. 54, 704–711.

Stammen, S., Müller, B.K., Korneli, C., Biedendieck, R., Gamer, M., Franco-Lara, E., and Jahn, D. (2010). High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium. Appl. Environ. Microbiol. 76, 4037–4046.

Vary, P. (1992). Development of genetic engineering in Bacillus megaterium. Biotechnol. Read. Mass 22, 251–310.

Vary, P.S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W.-D., and Jahn, D. (2007). Bacillus megaterium—from simple soil bacterium to industrial protein production host. Appl. Microbiol. Biotechnol. 76, 957–967.

Weigel, P.H. (2002). Functional Characteristics and Catalytic Mechanisms of the Bacterial Hyaluronan Synthases. IUBMB Life 54, 201–211.

Widner, B., Behr, R., Dollen, S.V., Tang, M., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P.L., Weigel, P.H., and Brown, S. (2005). Hyaluronic Acid Production in Bacillus subtilis. Appl. Environ. Microbiol. 71, 3747–3752.

Wittchen, K.D., and Meinhardt, F. (1995). Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl. Microbiol. Biotechnol. 42, 871–877.