Team:SJTU-BioX-Shanghai/Project

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="http://2014.igem.org/wiki/index.php?title=Team:SJTU-BioX-Shanghai/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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  <center><h2>Enzymes, in the name of crown, get closer!</h2></center> </div>
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<p>This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..</p></div>
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<a href="http://2014.igem.org/Team:SJTU-BioX-Shanghai/Part1_Connect" title="Connect">
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    <center><h2>Connection</h2></center><br/>
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    <center><img src="http://2014.igem.org/wiki/images/9/96/SJTU14_connect_small.png"></img></center></a>
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  <p>Want to know more about how to build the crown?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p>
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      <center><h2>Polymerization</h2></center><br/>
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      <center><img src="/wiki/images/5/51/SJTU14_Application_small.png"></img></center></a>
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    <p>What makes this little crown a real useful tool?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p>
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    <center><h2>TAL Improvement</h2></center><br/>
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  <p>We work for the better using of TAL!<br/><a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement">Click here~</a></p>
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<a href="http://2014.igem.org/Team:SJTU-BioX-Shanghai"style="color:#000000">Home </a> </td>
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<a href="http://2014.igem.org/Team:SJTU-BioX-Shanghai/Team"style="color:#000000"> Team </a> </td>
 
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<p>This year, we bring MembRing – an annular protein multimerization system in cytomembrane.</p>
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<p>This system will help us achieve the goal of selective polymerization of enzymes. Polymerized enzymes, different from normal scattered condition, has much more opportunities to contact with the substrates. Therefore, our project aims to increase as well as control the efficiency of complex reactions.</p>
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<p>The basic function, polymerization, is carried out by a circular DNA and DNA-binding protein to polymerize enzymes. First, a fluorescent protein, a transmembrane domain, an enzyme and a DNA binding protein are connected in order, forming a complex that the FP locates outside the film while the enzyme and DNA binding protein are in cytoplasm. Then integrate specific matching DNA sequences onto an exogenous plasmid and co-transform this connecter with expression vectors into E.Coli to associate enzymes.</p>
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<p>In addition, we plan to make the enzymes selectable by remolding the connecter with different recognition sequences and co-transforming it with expression vectors. This will help the whole project to work in all kinds of practical applications.
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<h3>References </h3>
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.
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It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.
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Latest revision as of 09:20, 8 July 2015

Enzymes, in the name of crown, get closer!

This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..

Connection


Want to know more about how to build the crown?
Click here~

Polymerization


What makes this little crown a real useful tool?
Click here~

TAL Improvement


We work for the better using of TAL!
Click here~