Overview

We have characterized and submitted 42 BioBricks which could either be used directly or serve as a universal tool readily for potential scientific or engineering use.
Those BioBricks could be divided into four groups.
1.BioBricks in Basic Parts are all basic components of the whole project. They can be assembled to carry out different tasks.
2.BioBricks in USB is our designed sequence. They can help us easily and quickly insert our target sequence and make a whole part.
3.BioBricks in application is our complete part.
4.BioBricks in New TAL is our new design TAL parts which are robust and perform better in Golden Gate method.

Basic Parts

Review previous parts

ssDsbA: SsDsbA is the signal recognition particle (SRP)-dependent signaling sequence of DsbA. SsDsbA-tagged proteins are exported to the periplasm through the SRP pathway. With ssDsbA fused to the N-terminus, fusion proteins with Lgt are expected to be anchored onto inner membrane of E.coli.
From: ssDsbA-PDZ Ligand-LGT-SH3 Ligand ( (BBa_K771002, SJTU-BioX-Shanghai)

Lgt: Phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) is an inner membrane protein act as an membrane anchor of E.coli with seven transmembrane segments and has been successfully overexpressed in E. coli without causing harm to cells.
From: ssDsbA-PDZ Ligand-LGT-SH3 Ligand (BBa_K771002, SJTU-BioX-Shanghai)

mRFP: Red Fluorescent Protein. To visualize the localization of fusion protein with fluorescence test , we added mRFP in the Connectee1 and placed it just after the ssDsbA.
From: Highly engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010, Antiquity )

FL3-TALE(BBa_K1453300)

This is a TALE protein with a flexible linker 3 before it.
Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, so the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTGGTCATGAGA(12bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.

Connector

We have four types of connector.

pBluescript II KS(+) ScaI deletion (BBa_K1453301)
pBluescript II KS(+) EcoRV deletion (BBa_K1453302)
pBluescript II KS(+)_3_copy (BBa_K1453303)
pBluescript II KS(+)_5_copy (BBa_K1453304)
Each type of connector has its own function. If you want to know the details, please click it. We have introduction on our part's main page.

USB

We make two kinds of USB. One is TAL USB, the other is Enzyme USB. They can help us easily and quickly insert our target TALE or Enzyme, respectively.

TAL USB