Team:SF Bay Area DIYbio/Notebook

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Notebook

General Protocols


  • Electra Cloning Protocol
  • DNA Handling Protocol
  • Media:Zymo E. coli competent cells transformation protocol.pdf
  • Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  • Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf

General Documentation Information

June 2014

August 2014

  • CLONE_082514_humKappaCasein(Kex+)
  • Growth Curve Experiment

September 2014

  • Cloning 7 casein gBlocks, 12Sep2014 and on
  • Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
  • Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  • Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014

October 2014

  • Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  • Cloning human alpha casein S1, 04Oct2014 and on
  • Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014