Team:SF Bay Area DIYbio/Notebook

From 2014.igem.org

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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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General Protocols</p>
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  <a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAHandling> DNA Handling </a> <br>
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    <a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ElectraCloningProtocol> Electra Cloning Protocol </a><br>
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    Media:Zymo E. coli competent cells transformation protocol.pdf<br>
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    Media:D4025i.pdf (Zyppy plasmid midiprep protocol)<br>
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    Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf <br>
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<p>General Documentation Information
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</p>
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    Real Vegan Cheese Notebook Documentation Guidelines<br>
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    Real Vegan Cheese Notebook Training Sign-off <br>
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<p>June 2014
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</p>
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    CR_062214_humanKappaCasein_001 <br>
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August 2014
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    CLONE_082514_humKappaCasein(Kex+)<br>
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    Growth Curve Experiment <br>
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September 2014
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    Cloning 7 casein gBlocks, 12Sep2014 and on<br>
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    Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br>
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    Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br>
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    Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br>
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October 2014
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    Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br>
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    Cloning human alpha casein S1, 04Oct2014 and on<br>
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    Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014<br>
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Revision as of 03:52, 14 October 2014



WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

General Protocols

Electra Cloning Protocol
Media:Zymo E. coli competent cells transformation protocol.pdf
Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf

General Documentation Information

Real Vegan Cheese Notebook Documentation Guidelines
Real Vegan Cheese Notebook Training Sign-off

June 2014

CR_062214_humanKappaCasein_001
August 2014 CLONE_082514_humKappaCasein(Kex+)
Growth Curve Experiment
September 2014 Cloning 7 casein gBlocks, 12Sep2014 and on
Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014 Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
Cloning human alpha casein S1, 04Oct2014 and on
Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014

Retrieved from "http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook"