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Team:SF Bay Area DIYbio/Notebook
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<p><b>September 2014</b></p><ul> | <p><b>September 2014</b></p><ul> | ||
<li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br> | <li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br> | ||
| - | <li><a href="https://2014.igem.org/troubleshooting12.9">Troubleshooting failed transformation of P.FAKS.humBeta.S on 12 Sept. 2014</a><br> | + | <li><a href="https://2014.igem.org/troubleshooting12.9">Troubleshooting failed transformation of P.FAKS.humBeta.S on <b>12 Sept. 2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/CloningBovineBetaCaesinSept">Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014</a><br> | + | <li><a href="https://2014.igem.org/CloningBovineBetaCaesinSept">Cloning bovine beta casein, Fam20C (Kex + & -), from <b>27Sep2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/Submitting10CaesinSept">Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014</a><br></ul></p><br> | + | <li><a href="https://2014.igem.org/Submitting10CaesinSept">Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing <b>30Sep2014</b></a><br></ul></p><br> |
<p><b>October 2014</b></p><ul> | <p><b>October 2014</b></p><ul> | ||
| - | <li><a href="https://2014.igem.org/InitialYeastStudyOct">Initial studies of yeast strains' growth conditions and troubleshooting</a><br> | + | <li><a href="https://2014.igem.org/InitialYeastStudyOct">Initial studies of yeast strains' growth conditions and troubleshooting <b>Oct.2</b></a><br> |
| - | <li><a href="https://2014.igem.org/RepeadMidiprepsOct">Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2</a><br> | + | <li><a href="https://2014.igem.org/RepeadMidiprepsOct">Repeat Midipreps to select new clones for sequencing for failed sequences from <b>Oct. 2</b></a><br> |
| - | <li><a href="https://2014.igem.org/CloningHumanAlphaS1Oct">Cloning human alpha casein S1, 04Oct2014</a><br> | + | <li><a href="https://2014.igem.org/CloningHumanAlphaS1Oct">Cloning human alpha casein S1, <b>04Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/CloningBovineKappaBetaOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014</a><br> | + | <li><a href="https://2014.igem.org/CloningBovineKappaBetaOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>08Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/SubmittingPlasmidSequencingOct">Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014</a><br> | + | <li><a href="https://2014.igem.org/SubmittingPlasmidSequencingOct">Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing <b>10Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/CloningBovineKappaBetaBioBrickOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014</a><br> | + | <li><a href="https://2014.igem.org/CloningBovineKappaBetaBioBrickOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>10Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/CloningBovineBetaOct">Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014</a><br> | + | <li><a href="https://2014.igem.org/CloningBovineBetaOct">Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>13Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/SubmittingFam20COct">Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014</a><br> | + | <li><a href="https://2014.igem.org/SubmittingFam20COct">Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers <b>14Oct2014</b></a><br> |
| - | <li><a href="https://2014.igem.org/SubmittingFam20CkexOct">Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014</a><br><br></ul></p> | + | <li><a href="https://2014.igem.org/SubmittingFam20CkexOct">Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing <b>15Oct2014</b></a><br><br></ul></p> |
</html> | </html> | ||
Revision as of 02:00, 17 October 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Notebook
General Protocols
- Electra Cloning Protocol
- DNA Handling Protocol
- Media:Zymo E. coli competent cells transformation protocol.pdf
- Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
- Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
General Documentation Information
June 2014
August 2014
September 2014
- Cloning 7 casein gBlocks, 12 Sep 2014
- Troubleshooting failed transformation of P.FAKS.humBeta.S on 12 Sept. 2014
- Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014
- Initial studies of yeast strains' growth conditions and troubleshooting Oct.2
- Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- Cloning human alpha casein S1, 04Oct2014
- Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014
- Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014
- Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014
- Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014
- Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014
- Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014
