From 2014.igem.org

Revision as of 23:51, 16 October 2014

WELCOME TO iGEM 2014! Your team has been approved and you are ready to start the iGEM season! On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! Click here to edit this page!

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Notebook

General Protocols

• Electra Cloning Protocol
  
• DNA Handling Protocol
  
• Media:Zymo E. coli competent cells transformation protocol.pdf
  
• Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  
• Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
  

General Documentation Information

• Real Vegan Cheese Notebook Documentation Guidelines
  

June 2014

• CR_062214_humanKappaCasein_001
  

August 2014

• CLONE_082514_humKappaCasein(Kex+)
  
• Growth Curve Experiment
  

September 2014

• Cloning 7 casein gBlocks, 12 Sep 2014
  
• Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
  
• Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  
• Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
  

October 2014

• Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  
• Cloning human alpha casein S1, 04Oct2014 and on
  
• Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014