Team:SCU-China/Transmitter
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- | <li>Note: The different concentrations are from different tubes.</p><p>2. The electrophoresis results are shown in Figure 2.</p>< | + | <li>Note: The different concentrations are from different tubes.</p><p>2. The electrophoresis results are shown in Figure 2.</p><img src="https://static.igem.org/mediawiki/2014/4/49/T2.png"><p>Figure 2. A, C, and D the gel purification results and cleaved plasmids. B. the cleaved EP21.</p><h1 id="4" style="padding-top: 80px; |
margin-top: -45px;">Week 4 8.11-8.17</h1><p>1. We did the liquid culture of BBa_R0077 and BBa_C0077.</p><p>2. We extracted the BBa_J37019, BBa_I9026 plasmids and then we cleaved both of them (BBa_J37019 with Spe I and Pst I; BBa_I9026 with Xba I and Pst I) and linked them with pSB1C3. Finally we tested the quality by transforming into E.coli.</p><p>3. We linked BBa_J37019 and BBa_I9026(LI1), BBa_R0077 and BBa_C0077 with pSB1C3.</p><p>4. We extracted BBa_I9026, BBa_J37019, BBa_C0077, and BBa_R0077.</p><p>5. We cleaved BBa_J37019 with E, S (D1, D2) or S, P (D3, D4) and BBa_I9026 plasmids with E, S, (D5, D6) and S, P (D7, D8) enzymes. Then we did the gel purification.</p><p>6. We linked the BBa_J37019 with BBa_I9026 with different types (named LI3).</p><p>7. We transformed LI3 into E.coli and extracted the plasmids to test the quality.</p><p>Results</p><table class="table table-striped"><caption>The concentration of plasmids</caption><thead><tr><td><p>Name</li> | margin-top: -45px;">Week 4 8.11-8.17</h1><p>1. We did the liquid culture of BBa_R0077 and BBa_C0077.</p><p>2. We extracted the BBa_J37019, BBa_I9026 plasmids and then we cleaved both of them (BBa_J37019 with Spe I and Pst I; BBa_I9026 with Xba I and Pst I) and linked them with pSB1C3. Finally we tested the quality by transforming into E.coli.</p><p>3. We linked BBa_J37019 and BBa_I9026(LI1), BBa_R0077 and BBa_C0077 with pSB1C3.</p><p>4. We extracted BBa_I9026, BBa_J37019, BBa_C0077, and BBa_R0077.</p><p>5. We cleaved BBa_J37019 with E, S (D1, D2) or S, P (D3, D4) and BBa_I9026 plasmids with E, S, (D5, D6) and S, P (D7, D8) enzymes. Then we did the gel purification.</p><p>6. We linked the BBa_J37019 with BBa_I9026 with different types (named LI3).</p><p>7. We transformed LI3 into E.coli and extracted the plasmids to test the quality.</p><p>Results</p><table class="table table-striped"><caption>The concentration of plasmids</caption><thead><tr><td><p>Name</li> | ||
Revision as of 03:47, 18 October 2014