Team:SCU-China/Digestion

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <style> h1[id] { padding-top: 80px; margin-top: -45px; } #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear{disp...")
 
Line 113: Line 113:
             <li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a></li>
             <li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a></li>
             <li><a href="https://2014.igem.org/Team:SCU-China/Team">Team</a></li>
             <li><a href="https://2014.igem.org/Team:SCU-China/Team">Team</a></li>
-
             <li class="dropdown   active"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Notbook<span class="caret"></span></a>
+
             <li class="dropdown active"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<span class="caret"></span></a>
               <ul class="dropdown-menu" role="menu">
               <ul class="dropdown-menu" role="menu">
                 <li class="dropdown-header">Notebook</li>
                 <li class="dropdown-header">Notebook</li>
-
                <li><a href="https://2014.igem.org/Team:SCU-China/Transmitter">Notebook of Transmitter</a></li>
+
                <li><a href="https://2014.igem.org/Team:SCU-China/Biobricks">Notebook of Biobricks</a></li>
 +
<li><a href="https://2014.igem.org/Team:SCU-China/Transmitter">Notebook of Transmitter</a></li>
                 <li><a href="https://2014.igem.org/Team:SCU-China/Effector">Notebook of Effector</a></li>
                 <li><a href="https://2014.igem.org/Team:SCU-China/Effector">Notebook of Effector</a></li>
                 <li><a href="https://2014.igem.org/Team:SCU-China/Sensor">Notebook of Sensor</a></li>
                 <li><a href="https://2014.igem.org/Team:SCU-China/Sensor">Notebook of Sensor</a></li>

Latest revision as of 18:56, 17 October 2014

Digestion Protocol

According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20μl).

DNA

250ng

Enzyme 1

1μl

Enzyme 2

1μl

10 X Buffer

2μl

H2O

Up to 20μl

Sichuan university