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Team:Purdue/Results/Wetlab Experiments
From 2014.igem.org
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| - | <p>Our | + | <p>Our two main wet lab projects were the transformation of Bacillus Subtilis and assembly of our plasmids using Gibson Assembly. |
We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis. </p> | We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis. </p> | ||
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| - | <p>We synthesized our | + | <p>We synthesized our two plasmids using Integrated DNA Technologies (IDT). We currently have them as gBlocks and are trouble shooting the Gibson Assembly.</p> |
Revision as of 01:31, 18 October 2014
Our two main wet lab projects were the transformation of Bacillus Subtilis and assembly of our plasmids using Gibson Assembly.
We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis. Paper
http://ac.els-cdn.com/S0167701207001315/1-s2.0-S0167701207001315-main.pdf?_tid=0f6ae27a-f585-11e3-a635-00000aacb361&acdnat=1402943939_6d4bbe673ecc5dfa11e7309507a2c4e8 We synthesized our two plasmids using Integrated DNA Technologies (IDT). We currently have them as gBlocks and are trouble shooting the Gibson Assembly.
Wet Lab Experiments
