Team:Paris Saclay/Notebook/September/18

From 2014.igem.org

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(=PCR with colony)
(PCR with colony)
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Only pps4 is positive for one clone and pps3 for one.
Only pps4 is positive for one clone and pps3 for one.
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[[File:1809 1-10 pps2 10-16 pps3.jpg|400px]]
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well 2-11 : pPS2 
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well 12-16 : pPS3
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[[File:1809 pps3 17-20 21 pps4.jpg|400px]]
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well 2-4 pPS3
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well 5 pPS4
====liquide culture====
====liquide culture====
liquide culture of the positiv clones
liquide culture of the positiv clones

Revision as of 12:10, 26 September 2014

Contents

Lab Work

By Mélanie

D-Lemon scent

PCR with colony

clones obtained after the ligation in PPSI have been tested by PCR to see if we have an insert: We have tested pps2-pps3 pps4.


Only pps4 is positive for one clone and pps3 for one.

1809 1-10 pps2 10-16 pps3.jpg

well 2-11 : pPS2

well 12-16 : pPS3

1809 pps3 17-20 21 pps4.jpg

well 2-4 pPS3 well 5 pPS4

liquide culture

liquide culture of the positiv clones