body{font-family:'Open Sans',sans-serif;font-size:13px}header{box-shadow:0 6px 6px rgba(0,0,0,.3)}header .container{height:80px}header .logo{padding:10px 0 10px 80px}header .igem{position:absolute;top:20px;right:-50px;z-index:100}header .igem img{width:40px;height:40px}header ul{float:right;list-style:none}header ul li{display:inline-block;white-space:nowrap}header ul li a{display:block;padding:0 15px;height:80px;line-height:80px;color:#000;text-decoration:none!important;transition:background-color .2s linear}header ul li a:active,header ul li a:hover,header ul li a:visited{background-color:#ecffeb;text-decoration:none!important;color:#000}header ul.dropdown-menu li{display:block}header ul.dropdown-menu li a{height:auto;padding:6px 20px;text-decoration:none}section{padding:50px 0}section h1{font-weight:300;font-size:48px;padding:0 0 50px}.dropdown-menu{margin:0}ul.menu li.dropdown:hover>ul.dropdown-menu{display:block}.bx-wrapper img{display:block;max-width:100%;min-width:970px;margin:0 auto}.welcome{padding:80px 0;background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.about{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.about h2{font-size:18px;font-weight:700}.about p.project{font-size:14px;padding:10px}.about p.project:last-of-type{padding-bottom:40px}.about .row:first-of-type{margin-bottom:40px}.about .col-md-4 i{transition:color .2s linear}.about .col-md-4:hover i{color:#2ECC71}.team{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png);transition:all .3s linear;padding:0 0 40px}.team .img-responsive{border-radius:20em;-webkit-border-radius:20em;-moz-border-radius:20em;-webkit-filter:grayscale(100%);-moz-filter:grayscale(100%);-ms-filter:grayscale(100%);filter:grayscale(100%);filter:gray;transition:all .3s linear;max-width:200px;margin:0 auto}.team .col-md-4:hover .img-responsive{-webkit-filter:none;-moz-filter:none;-ms-filter:none;filter:none}.team .row:first-of-type{margin-bottom:40px}.team h2{font-size:18px;font-weight:700;color:#019875}.team h3{font-size:13px;font-weight:700;color:#019875}.team .col-md-3{padding:0 30px}.team .meetourteam{margin-bottom:60px}.social{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.sponsors .col-md-2{display:table;height:90px}.sponsors .middle{display:table-cell;vertical-align:middle}.sponsors .row:first-of-type{margin-bottom:40px}.general{padding-top:0;background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.title{background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.title h1,.title h2{text-align:center}.title h1{margin-top:0;padding:40px 0;margin-bottom:40px}.title h2{font-size:18px;color:#999;margin:0 0 50px;padding-bottom:40px}.general p{margin:0 0 40px}.fa-8x{font-size:8em;color:#019875}.row .boxed-headline{width:100%;height:200px;background-color:#90C695}.container .overview{padding-bottom:40px}footer{color:#444;background-color:#F2F1EF;padding:50px 0}footer ul.sponsors{float:left;width:100%;padding:0;margin:20px 0 0;list-style-type:none}footer ul.sponsors li{display:inline;padding:0 20px}footer ul.sponsors li img{width:90px}#top-section{border:0;height:14px;z-index:100;top:0;position:fixed;width:975px;left:50%;margin-left:-487px;background-color:transparent}#content,#globalWrapper{width:100%;height:100%;border:0;background-color:transparent;margin:0;padding:0;font-size:100%}#catlinks,#contentSub,#footer-box,#p-logo,#search-controls,#sitesub,.firstHeading,.printfooter,.visualClear{display:none}h1,h2,h3,h4,h5,h6{border-bottom:none}#bodyContent h1,#bodyContent h2{margin-bottom:40px}

             <img src="https://static.igem.org/mediawiki/2014/e/e0/Uea_logo.png" class="logo"/>

               <a href="https://2014.igem.org/Main_Page"/><img src="https://static.igem.org/mediawiki/2014/a/a3/Uea_igem-logo.png"/></a>

                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Science-Cafe">Science Cafe</a></li>

                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Safety_Forms">Safety Forms</a></li>

 

We have used <i>E. coli</i> as a chassis organism to allow us to clone our gene construct successfully. After using Golden Gate cloning to make our Level 1 and Level 2 constructs, we needed to transform E. coli with the newly made constructs. This is to ensure that we have enough of the correct construct when we get to the next stage of transforming Agrobacterium tumefaciens.

<i>A. Tumefaciens</i> (Agro) was a chassis organism in this project to act as a shuttle chassis for the delivery of our Level 1 and Level 2 DNA constructs into Nicotiana benthamiana. Agro infects the plant through its Ti plasmid. This inserts a segment of its DNA, T-DNA, into the host’s genome. This allows the expression of the gene of interest.

<i>N. benthamiana</i> is a widely used experimental plant from the solanaceous group of flowering plants that includes tomatoes, potatoes and capsicums. It is widely used in plant pathology due to the large number of plant pathogens (viruses, bacteria, fungi, oomycetes etc) that can successfully infect it. Of importance to synthetic biologists,<i> N. benthamiana </i> it is easily genetically transformed and regenerated and amenable to facile methods for virus-induced gene silencing and transient protein expression including the production of therapeutic compounds and pharmaceuticals. We selected it as the plant host as it is a model organism for the plant world.