Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

(Difference between revisions)
Line 77: Line 77:
<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p>
<p>
<p>
-
Transformation of NCTU1 (G1)</p>
+
<font color="yellow">Transformation of</font> NCTU1 (G1)</p>
<p>
<p>
-
Transformation of PBAN (G2)</p>
+
<font color="yellow">Transformation of</font> PBAN (G2)</p>
<p>
<p>
<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p>
<p>
<p>
-
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
+
<font color="yellow">Cultivation of</font> [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
<p>
<p>
-
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p>
+
<font color="yellow">Cultivation of </font>[RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p>
<p>
<p>
-
Mini-prep of [Pcons]Ar</p>
+
<font color="yellow">Mini-prep of</font> [Pcons]Ar</p>
<p>
<p>
-
Digestion of [Pcons]Ar (ES) (G2)</p>
+
<font color="yellow">Digestion of</font> [Pcons]Ar (ES) (G2)</p>
<p>
<p>
<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
<p>
<p>
-
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
+
<font color="yellow">Mini-prep of</font> [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
<p>
<p>
-
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
+
<font color="yellow">Digestion of</font> [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
<p>
<p>
-
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)</p>
+
<font color="yellow">Electrophoresis of</font> [Pcons](mini)&(dig ES),digestion of [Pcons](XP)
 +
</p><p>
 +
<font color="yellow">Ligation of</font>[Pcons]+[RBS+BFP+J61048] (G2)</p>
<p>  
<p>  
<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
<p>
<p>
-
Electrophoresis of plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
+
<font color="yellow">Electrophoresis of</font> plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
<p>
<p>
-
Purification from electrophoresis of[PBAN]No.4&5&6(G1)</p>
+
<font color="yellow">Purification from electrophoresis of</font>[PBAN]No.4&5&6(G1)</p>
<p>
<p>
-
Transformation of the ligation product made yesterday</p>
+
<font color="yellow">Transformation of</font> the ligation product made yesterday</p>
<p>
<p>
-
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p>
+
<font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p>
<p>
<p>
<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
<p>
<p>
-
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
+
<font color="yellow">Digestion of</font> purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
<p>
<p>
-
Purification from electrophoresis of [PBAN]No.4&5&6</p>
+
<font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6</p>
<p>
<p>
-
Transformation of [B0034]Ar (G2)</p>
+
<font color="yellow">Transformation of</font> [B0034]Ar (G2)</p>
<p>  
<p>  
<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p>
<p>
<p>
-
Electrophoresis of the purification products from electrophoresis and digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
+
<font color="yellow">Electrophoresis of</font> the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
<p>
<p>
-
PCR of [B0034]</p>
+
<font color="yellow">PCR of</font> [B0034]</p>
<p>
<p>
-
Electrophoresis of[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p>
+
<font color="yellow">Electrophoresis of</font>[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p>
<p>
<p>
-
Mini-prep of [B0034]Ar E.coli</p>
+
<font color="yellow">Mini-prep of</font> [B0034]Ar E.coli</p>
<p>
<p>
-
Ligation of [Pcons]+[B0034] +PSB1K3</p>
+
<font color="yellow">Ligation of</font> [Pcons]+[B0034] +PSB1K3</p>
<p>
<p>
-
Digestion of [BFP](ES)</p>
+
<font color="yellow">Digestion of</font> [BFP](ES)</p>
<p>
<p>
-
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p>
+
<font color="yellow">Electrophoresis of </font>plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p>
<br></div>
<br></div>
<div  class="single note">
<div  class="single note">
Line 137: Line 139:
<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p>
<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p>
<p>
<p>
-
Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
+
<font color="yellow">Electrophoresis of</font> the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
<br></div>
<br></div>
<div  class="single note">
<div  class="single note">

Revision as of 13:17, 14 October 2014

  • Sunday
  • Monday
  • Tuesday
  • Wednesday
  • Thursday
  • Friday
  • Saturday
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None

None

8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [Pcons]Ar

Digestion of [Pcons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)

Ligation of[Pcons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of[PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [Pcons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])


8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)


None

None

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