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Team:NCTU Formosa/Notebook/test5
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Ligation:[Pcons]+[B0034] +PSB1K3 | Ligation:[Pcons]+[B0034] +PSB1K3 | ||
Digestion of [BFP](ES) | Digestion of [BFP](ES) | ||
| - | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons] | + | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p> |
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Revision as of 16:01, 9 October 2014
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8/11 Transformation of NCTU1 (G1) Transformation of PBAN (G2)
8/12 Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr Mini-prep of [Pcons]Ar Digestion of [Pcons]Ar (ES) (G2)
8/13 Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
8/14 Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6(G1) Transformation of the ligation product made yesterday Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15 Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6 Transformation of [B0034]Ar (G2)
8/16 Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) PCR:[B0034] Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034 Mini-prep of [B0034]Ar E.coli Ligation:[Pcons]+[B0034] +PSB1K3 Digestion of [BFP](ES) Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])
