Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

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8/11
8/11
Transformation of NCTU1 (G1)
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
Transformation of PBAN (G2)
-
+
</p>
 +
<p>
8/12
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
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Mini-prep of [Pcons]Ar
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
Digestion of [Pcons]Ar (ES) (G2)
-
+
</p>
 +
<p>
8/13
8/13
Mini-prep of  [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Mini-prep of  [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
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+
</p>
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<p>
8/14
8/14
Electrophoresis of plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Electrophoresis of plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
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Transformation of the ligation product made yesterday
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
-
+
</p>
 +
<p>
8/15
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis:[PBAN]No.4&5&6
Purification from electrophoresis:[PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
Transformation of [B0034]Ar (G2)
-
+
</p>
 +
<p>
8/16
8/16
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)
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Ligation:[Pcons]+[B0034] +PSB1K3
Ligation:[Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Digestion of [BFP](ES)
-
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)
+
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)</p>
<br></div>
<br></div>
<div  class="single note">
<div  class="single note">

Revision as of 15:57, 9 October 2014

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8/11 Transformation of NCTU1 (G1) Transformation of PBAN (G2)

8/12 Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr Mini-prep of [Pcons]Ar Digestion of [Pcons]Ar (ES) (G2)

8/13 Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)

8/14 Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6(G1) Transformation of the ligation product made yesterday Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)

8/15 Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6 Transformation of [B0034]Ar (G2)

8/16 Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) PCR:[B0034] Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034 Mini-prep of [B0034]Ar E.coli Ligation:[Pcons]+[B0034] +PSB1K3 Digestion of [BFP](ES) Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)


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