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Team:NCTU Formosa/Notebook/test5
From 2014.igem.org
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| - | + | 8/11 | |
| + | Transformation of NCTU1 (G1) | ||
| + | Transformation of PBAN (G2) | ||
| + | |||
| + | 8/12 | ||
| + | Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) | ||
| + | Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr | ||
| + | Mini-prep of [Pcons]Ar | ||
| + | Digestion of [Pcons]Ar (ES) (G2) | ||
| + | |||
| + | 8/13 | ||
| + | Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) | ||
| + | Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) | ||
| + | Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2) | ||
| + | |||
| + | 8/14 | ||
| + | Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) | ||
| + | Purification from electrophoresis:[PBAN]No.4&5&6(G1) | ||
| + | Transformation of the ligation product made yesterday | ||
| + | Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2) | ||
| + | |||
| + | 8/15 | ||
| + | Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) | ||
| + | Purification from electrophoresis:[PBAN]No.4&5&6 | ||
| + | Transformation of [B0034]Ar (G2) | ||
| + | |||
| + | 8/16 | ||
| + | Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) | ||
| + | PCR:[B0034] | ||
| + | Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034 | ||
| + | Mini-prep of [B0034]Ar E.coli | ||
| + | Ligation:[Pcons]+[B0034] +PSB1K3 | ||
| + | Digestion of [BFP](ES) | ||
| + | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2) | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
Revision as of 15:54, 9 October 2014
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8/11
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
8/13
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
8/14
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Purification from electrophoresis:[PBAN]No.4&5&6(G1)
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis:[PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
8/16
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)
PCR:[B0034]
Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034
Mini-prep of [B0034]Ar E.coli
Ligation:[Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)
3
4
