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| Line 60: |
Line 60: |
| | <div class="single note">none<br></div> | | <div class="single note">none<br></div> |
| | <div class="single note"> | | <div class="single note"> |
| - | <p>4/11
| + | 1 |
| - | Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
| + | |
| - | Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
| + | |
| - | Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
| + | |
| - | <p>
| + | |
| - | <p>4/12
| + | |
| - | Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
| + | |
| - | Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
| + | |
| - | Cultivation of [mGFP] Ar (G3)
| + | |
| - | </p>
| + | |
| | <br></div> | | <br></div> |
| - | <div class="single note"> | + | <div class="single note"> |
| - | | + | 2 |
| - | <p>4/13
| + | |
| - | Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3)
| + | |
| - | Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
| + | |
| - | Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p>
| + | |
| - | | + | |
| - | <p>4/17
| + | |
| - | Transform of [PSB1K3] Kr (G1)</p>
| + | |
| - | | + | |
| - | <p>4/18
| + | |
| - | Cultivation of [PSB1K3] Kr (G1) </p>
| + | |
| - | | + | |
| - | <p>4/19
| + | |
| - | Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3)
| + | |
| - | Mini-prep of [PSB1K3] Kr(G1) </p>
| + | |
| - | | + | |
| - | | + | |
| | <br></div> | | <br></div> |
| - | <div class="single note"> | + | <div class="single note"> |
| - | <p>4/20
| + | 3 |
| - | Transform of [PSB1A3] Ar (G2)
| + | |
| - | Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p>
| + | |
| - | | + | |
| - | <p>4/21
| + | |
| - | Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
| + | |
| - | | + | |
| - | <p>4/22
| + | |
| - | Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
| + | |
| | <br></div> | | <br></div> |
| - | <div class="single note"> | + | <div class="single note"> |
| - | <p>
| + | 4 |
| - | 4/28 | + | |
| - | Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
| + | |
| - | Cultivation of [PSB1C3] Cr (G1)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 4/29
| + | |
| - | Mini-prep of [PSB1C3] Cr(G1)
| + | |
| - | Mini-prep of [PSB1C3]Cr (G2)
| + | |
| - | Transform of [B0032]Ar, and [BFP]Cr (G2)
| + | |
| - | Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
| + | |
| - | Cultivation of [B0032]Ar, and [BFP]Cr (G2)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 4/30
| + | |
| - | Mini-prep of [PSB1A3] Ar (G1)
| + | |
| - | Mini-prep of [B0032]Ar, and [BFP]Cr (G2)
| + | |
| - | Transform of [B0032]Ar, and [BFP]Cr, [T7promoter]ArKr and [T7 polymerase]Cr (G2)
| + | |
| - | Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
| + | |
| - | Digestion of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
| + | |
| - | Electrophoresis of plasmid and digested prouct of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
| + | |
| - | </p>
| + | |
| | <br></div> | | <br></div> |
| - |
| |
| | </div> | | </div> |
| | <div class="clear"></div> | | <div class="clear"></div> |
| | </div> | | </div> |
"
