Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

(Difference between revisions)

Latest revision as of 14:24, 17 October 2014

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8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [P_cons]Ar

Digestion of [P_cons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [P_cons](mini)&(dig ES),digestion of [P_cons](XP)

Ligation of [P_cons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[P_cons],[P_cons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of [PSB1K3](mini)&(dig E),[P_cons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [P_cons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P_cons])

8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)

None

@@ Line 87: / Line 87: @@
 <font color="yellow">Cultivation of </font> [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p>
 <p>
-<font color="yellow">Mini-prep of</font> [Pcons]Ar</p>
+<font color="yellow">Mini-prep of</font> [P<sub>cons</sub>]Ar</p>
 <p>
-<font color="yellow">Digestion of</font> [Pcons]Ar (ES) (G2)</p>
+<font color="yellow">Digestion of</font> [P<sub>cons</sub>]Ar (ES) (G2)</p>
 <p>
 <font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
@@ Line 97: / Line 97: @@
 <font color="yellow">Digestion of</font> [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
 <p>
-<font color="yellow">Electrophoresis of</font> [Pcons](mini)&(dig ES),digestion of [Pcons](XP)
+<font color="yellow">Electrophoresis of</font> [P<sub>cons</sub>](mini)&(dig ES),digestion of [P<sub>cons</sub>](XP)
 </p><p>
-<font color="yellow">Ligation of</font> [Pcons]+[RBS+BFP+J61048] (G2)</p>
+<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[RBS+BFP+J61048] (G2)</p>
 <p>
 <font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
@@ Line 109: / Line 109: @@
 <font color="yellow">Transformation of</font> the ligation product made yesterday</p>
 <p>
-<font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p>
+<font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[P<sub>cons</sub>],[P<sub>cons</sub>+RBS+BFP+J61048] (G2)</p>
 <p>
 <font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
@@ Line 125: / Line 125: @@
 <font color="yellow">PCR of</font> [B0034]</p>
 <p>
-<font color="yellow">Electrophoresis of</font> [PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p>
+<font color="yellow">Electrophoresis of</font> [PSB1K3](mini)&(dig E),[P<sub>cons</sub>](dig SP),PCR products of B0034</p>
 <p>
 <font color="yellow">Mini-prep of</font> [B0034]Ar E.coli</p>
 <p>
-<font color="yellow">Ligation of</font> [Pcons]+[B0034] +PSB1K3</p>
+<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[B0034] +PSB1K3</p>
 <p>
 <font color="yellow">Digestion of</font> [BFP](ES)</p>
 <p>
-<font color="yellow">Electrophoresis of </font> plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p>
+<font color="yellow">Electrophoresis of </font> plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P<sub>cons</sub>])</p>
 <br></div>
 <div  class="single note">