Team:NCTU Formosa/Notebook/test4

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7/1 Ligation of [J23101+B0032+PSB1C3] Cr (G1) Digestion of [B0032] Ar Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/2 Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1) Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)

7/3 Mini-prep of [B0032+BFP+J61048] and then digestion: (XP) Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/4 Digestion of [T7 promoter](SP) (G2) Cultivation of [J23101] Ar (G3)

7/5 PCR:[B0032+K592100+J61048*11] Ar (G1) Electrophoresis of PCR products (G1) Ligation of [J23101+B0032+PSB1C3*3] Cr (G1) Electrophoresis of plasmid and digestion products:[T7promoter](SP),[B0031+BFP+J61048](XP) Ligation:[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)

7/6 Transform the ligation products made at 7/5 (G2)


7/7 Transformation of [J23101+B0032*5] Cr (G1) Cultivation of [B0032+K592100+J61048*2] Ar (G1) Cultivation of [J23101] Ar (G3) Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)

7/8 Mini-prep of [B0032+K592100+J61048*2] Ar and then digest only one of two: (XP) (G1) PCR:[T7+B0032+BFP+J61048] (G2) Digestion of [J23101] Ar (ES) (G3)

7/9 Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)

7/10 Mini-prep of [B0032+BFP+J61048]Cr Digestion of [B0032+FP+J61048]Cr( XP) Electrophoresis of plasmid and digestion products [B0032+BFP+J61048]Cr (XP) (G2)

7/12 Digestion of [B0032+BFP+J612048](XP), [T7+B0032+BFP+J61048]Kr (XP) (G2)

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7/14 Electrophoresis of [B0032+BFP+J61048]Cr,[T7+B0032+BFP+J61048]Kr (G2)

7/15 Cultivation of [B0032+BFP+J61048]Cr,[PT7+B0032+BFP+J61048]Kr E.coli in LB broth (G2)

7/16 Mini-prep of [B0032+BFP+J61048],[PT7+B0032+BFP+J61048],and then made the Digestion[B0032+BFP+J61048]Cr(XP),[PT7+B032+BFP+J61048]Kr(ES) (G2)

7/17 Electrophoresis of the plasmid and digestion products made yesterday (G2)

7/19 PCR:[J23101+B0032*12] Cr (G1) Electrophoresis of the PCR products, [B0032+K592100+J61048*2] Ar ,digestion product of [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)

7/20 Digestion of [B0032+K592100+J61048] Ar (EP) (G1) Digestion of [J23101] Ar (ES) (G3) Ligation of [B0032+K592100+J61048] Cr (G1) Ligation of [J23101+B0032+PSB1K3] Kr (G3) PCR:[J23101+B0032*4] Cr (G1) Cultivation of [J61048+B0032*4]Cr E.coli in LB broth (G1) Electrophoresis of the PCR products, digestion products of [J23101] Ar (SP) and [B0032+K592100+J61048] Ar (EP) (G1)



7/21 Transformation of ligation product made yesterday (G1) Transformation of ligation product [J23101+B0032+PSB1K3] Kr (G3) Ligation of [mGFP+J61048+PSB1K3] Kr,[mGFP+B0015+PSB1K3] Kr (G3)

7/23 Cultivation of [B0032+K592100+J61048] Cr E.coli in LB broth (G1) Digestion of [J23101+B0032*3] Cr (EP) (G1) Digestion of [B0032+K592100+J61048] Ar (EP) (G1) Ligation of [J23101+B0032] Cr (G1) Mini-prep of [B0032+K592100+J61048] Cr Transformation of [B0032+K592100+J61048] Cr and [J23101+B0032] Ar (G1)

7/24 PCR: [B0032+K592100+J61048*12] Cr (G1) Electrophoresis of digestion products [J23101+B0032*3] Cr (EP) and the PCR products (G1) Transformation of [J23101+B0032] Cr (G1) Digestion of [BFP] Cr (ES), [B0015] Ar (XP), [J61048] Ar (XP), [B0032] Ar (XP), [BFP+B00315*2] Kr (XP) (G3)

7/26 Cultivation of [J23101+B0032*4] Cr E.coli in LB broth (G1) Digestion of [J23101] Ar (SP), [B0032] Ar (XP) (G3)

7/27 Ligation of [J23101+B0032+PSB1C3] Cr (G3)


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