Team:NCTU Formosa/Notebook/test4

From 2014.igem.org

(Difference between revisions)
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<div  class="single note">
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<p>5/4
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<p>7/3
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Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
+
Mini-prep of [B0032+BFP+J61048] and then digestion: (XP)
-
Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)
+
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
-
Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)
+
-
Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)
+
-
Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)
+
-
Transform of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)
+
-
Transform of [LacI] Ar (G3)
+
-
Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)
+
-
Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)
+
-
Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)
+
-
Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar  (G3)
+
</p>
</p>
<p>
<p>
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5/5
+
7/4
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Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)
+
Digestion of [T7 promoter](SP) (G2)
-
Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
+
Cultivation of [J23101] Ar (G3)
-
Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)
+
-
Electrophoresis of digested product [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)
+
</p>
</p>
<p>
<p>
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5/6
+
7/5
-
Mini-prep of [LacI] Ar (G3)
+
PCR:[B0032+K592100+J61048*11] Ar (G1)
-
</p>
+
Electrophoresis of PCR products (G1)
-
<p>
+
Ligation of [J23101+B0032+PSB1C3*3] Cr (G1)
-
 
+
Electrophoresis of plasmid and digestion products:[T7promoter](SP),[B0031+BFP+J61048](XP)   
-
5/7
+
Ligation:[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)
-
Transform of [PT7polymerase]Cr and [J61048]Ar (G2)
+
</p><p>7/6
 +
Transform the ligation products made at 7/5 (G2)
</p><p>
</p><p>
-
 
+
7/7
-
5/8
+
Transformation of [J23101+B0032*5] Cr (G1)
-
Transform of [B0015*3] Ar and [J61048*2] Ar (G2)
+
Cultivation of [B0032+K592100+J61048*2] Ar (G1)
-
Transform of [B0015] Ar and [J61048] Ar (G3)
+
Cultivation of [J23101] Ar (G3)
-
Transform of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1)
+
Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)
-
Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2)
+
</p><p>
-
Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2)
+
7/8
-
Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
+
Mini-prep of [B0032+K592100+J61048*2] Ar and then digest only one of two: (XP) (G1)
-
Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
+
PCR:[T7+B0032+BFP+J61048] (G2)
 +
Digestion of [J23101] Ar (ES) (G3)
 +
</p><p>
 +
7/9
 +
Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)
</p><p>
</p><p>
-
 
-
5/9
 
-
Cultivation of [B0015] Ar, [J61048] Ar  [LacI] Ar (G3)
 
-
</p>
 
-
<p>
 
-
5/10
 
-
Mini-prep [LacI] Ar (G3)
 
-
</p>
 
-
 
<br></div>
<br></div>
<div class="single note">
<div class="single note">

Revision as of 13:28, 9 October 2014

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7/1 Ligation of [J23101+B0032+PSB1C3] Cr (G1) Digestion of [B0032] Ar Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/2 Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1) Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)


7/3 Mini-prep of [B0032+BFP+J61048] and then digestion: (XP) Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/4 Digestion of [T7 promoter](SP) (G2) Cultivation of [J23101] Ar (G3)

7/5 PCR:[B0032+K592100+J61048*11] Ar (G1) Electrophoresis of PCR products (G1) Ligation of [J23101+B0032+PSB1C3*3] Cr (G1) Electrophoresis of plasmid and digestion products:[T7promoter](SP),[B0031+BFP+J61048](XP) Ligation:[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)

7/6 Transform the ligation products made at 7/5 (G2)

7/7 Transformation of [J23101+B0032*5] Cr (G1) Cultivation of [B0032+K592100+J61048*2] Ar (G1) Cultivation of [J23101] Ar (G3) Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)

7/8 Mini-prep of [B0032+K592100+J61048*2] Ar and then digest only one of two: (XP) (G1) PCR:[T7+B0032+BFP+J61048] (G2) Digestion of [J23101] Ar (ES) (G3)

7/9 Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)


5/11 Cultivation of [J61048] Ar (G2) (ES) Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP) Transform of [T7 promoter] Kr (G2)

5/12 Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)

5/13 Transform of [T7 promoter] Kr (G2)

5/15 Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2) Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1) Mini-prep of [PSB1K3]Kr (G1) Digestion of [PSB1K3]Kr (EP) (G1)


5/19 Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)

5/20 Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2) Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1) Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)

5/21 Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2) Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1) Ligation of [K592100+J61048+PSB1K3] Kr (G1)

5/22 Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)

5/23 Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)

5/24 Digestion of [J61048] Ar (XP) (G2) PCR: [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2) Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)


5/27 Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2) Cultivation of [PSB1K3*3] Kr(G2)

5/28 PCR:[PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1) Mini-prep of [PSB1K3*3] Kr(G2) Digestion of [PSB1K3*3] Kr (EP) (G2) Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)

5/29 Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Transform of [J23101] Ar (G1) Transform of [T7 promoter] Ar Kr (G2) Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2) Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2) Ligation of [Plac+B0032+PSB1K3] Kr (G3)

5/30 Transform of ligation product (G2) Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3)


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