Team:NCTU Formosa/Notebook/test4

From 2014.igem.org

(Difference between revisions)
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</div>
</div>
<div class="calendar-note">
<div class="calendar-note">
-
<div class="single note">none<br></div>
+
<div class="single note">
 +
<p>
 +
5/1
 +
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
 +
Transform of [T7 polymerase]Cr (G2)</p>
 +
 
 +
<p>5/2
 +
Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
 +
Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
 +
Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p>
 +
 
 +
<p>5/3
 +
Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
 +
Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)
 +
Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)
 +
Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP),  [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and  B0015*2(ES & XP) (G2)
 +
Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)
 +
Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
 +
Ligation: [BFP+B0032+PSB1K3]Kr (G2)
 +
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
 +
Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2)
 +
Transform of [T7polymerase]Cr and [B0015]Ar (G2)</p><br></div>
<div  class="single note">
<div  class="single note">
-
<p>4/11
+
<p>5/4
-
Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
+
Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
-
Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)    
+
Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)
-
Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr  (G1)
+
Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)
 +
Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)
 +
Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)
 +
Transform of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)
 +
Transform of [LacI] Ar (G3)
 +
Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)
 +
Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)
 +
Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)
 +
Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
 +
</p>
<p>
<p>
-
<p>4/12
+
5/5
-
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
+
Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)
-
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
+
Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
-
Cultivation of [mGFP] Ar (G3)
+
Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)
 +
Electrophoresis of digested product [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)
</p>
</p>
-
<br></div>
+
<p>
-
<div class="single note">
+
5/6
 +
Mini-prep of [LacI] Ar (G3)
 +
</p>
 +
<p>
-
<p>4/13
+
5/7
-
Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3)
+
Transform of [PT7polymerase]Cr and [J61048]Ar (G2)
-
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
+
</p><p>
-
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p>
+
-
<p>4/17
+
5/8
-
Transform of [PSB1K3] Kr (G1)</p>
+
Transform of [B0015*3] Ar and [J61048*2] Ar (G2)
-
 
+
Transform of [B0015] Ar and [J61048] Ar (G3)
-
<p>4/18
+
Transform of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1)
-
Cultivation of [PSB1K3] Kr (G1) </p>
+
Mini-prep of  [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2)
-
 
+
Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2)
-
<p>4/19
+
Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
-
Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3)
+
Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
-
Mini-prep of [PSB1K3] Kr(G1) </p>
+
</p><p>
 +
5/9
 +
Cultivation of [B0015] Ar, [J61048] Ar  [LacI] Ar (G3)
 +
</p>
 +
<p>
 +
5/10
 +
Mini-prep [LacI] Ar (G3)
 +
</p>
<br></div>
<br></div>
<div class="single note">
<div class="single note">
-
<p>4/20
+
<p>5/11
-
Transform of [PSB1A3] Ar (G2)
+
Cultivation of [J61048] Ar (G2) (ES)
-
Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p>
+
Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)
 +
Transform of [T7 promoter] Kr (G2)
 +
</p>
 +
<p>
 +
5/12
 +
Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES)  (G2)
 +
</p>
 +
<p>
 +
5/13
 +
Transform of [T7 promoter] Kr (G2)
 +
</p>
 +
<p>
 +
5/15
 +
Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)
 +
Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)
 +
Mini-prep of [PSB1K3]Kr (G1)
 +
Digestion of [PSB1K3]Kr (EP) (G1)
 +
</p>
 +
 
 +
 
-
<p>4/21
 
-
Cultivation of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
 
-
<p>4/22
 
-
Mini-prep of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
 
<br></div>
<br></div>
<div class="single note">
<div class="single note">
<p>
<p>
-
4/28
+
5/19
-
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
+
Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)
-
Cultivation of [PSB1C3] Cr (G1)
+
</p><p>
 +
5/20
 +
Transform of  [T7polymerase] Kr and [PSB1K3] Kr (G2)
 +
Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)
 +
Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)
 +
Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)
</p>
</p>
<p>
<p>
-
4/29
+
5/21
-
Mini-prep of [PSB1C3] Cr(G1)
+
Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)
-
Mini-prep of [PSB1C3]Cr (G2)
+
Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)
-
Transform of [B0032]Ar, and [BFP]Cr (G2)
+
Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)
-
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
+
Ligation of [K592100+J61048+PSB1K3] Kr (G1)
-
Cultivation of [B0032]Ar, and [BFP]Cr (G2)
+
</p>
</p>
<p>
<p>
-
4/30
+
5/22
-
Mini-prep of [PSB1A3] Ar (G1)
+
Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)
-
Mini-prep of [B0032]Ar, and [BFP]Cr (G2)
+
-
Transform of [B0032]Ar, and [BFP]Cr, [T7promoter]ArKr and [T7 polymerase]Cr (G2)
+
-
Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
+
-
Digestion of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
+
-
Electrophoresis of plasmid and digested prouct of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
+
</p>
</p>
 +
<p>
 +
5/23
 +
Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)
 +
</p>
 +
<p>
 +
5/24
 +
Digestion of [J61048] Ar (XP) (G2)
 +
PCR: [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)
 +
Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar  (G2)
 +
</p>
 +
<br></div>
<br></div>
 +
<div class="single note">
 +
<p>
 +
5/27
 +
Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
 +
Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)
 +
Cultivation of [PSB1K3*3] Kr(G2)
 +
</p>
 +
<p>
 +
5/28
 +
PCR:[PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)
 +
Mini-prep of [PSB1K3*3] Kr(G2)
 +
Digestion of  [PSB1K3*3] Kr (EP) (G2)
 +
Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)
 +
</p>
 +
<p>
 +
 +
5/29
 +
Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
 +
Transform of [J23101] Ar (G1)
 +
Transform of [T7 promoter] Ar Kr (G2)
 +
Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)
 +
Ligation of  [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)
 +
Ligation of  [Plac+B0032+PSB1K3]  Kr (G3)
 +
</p>
 +
<p>
 +
5/30
 +
Transform of ligation product (G2)
 +
Transform of ligation product [Plac+B0032+PSB1K3]  Kr (G3)
 +
</p>
 +
<br></div>
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
</div>
</div>

Revision as of 13:20, 9 October 2014

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5/1 Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Transform of [T7 polymerase]Cr (G2)

5/2 Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)

5/3 Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3) Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1) Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2) Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1) Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1) Ligation: [BFP+B0032+PSB1K3]Kr (G2) Ligation of [J23101+B0032+PSB1C3] Cr (G1) Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2) Transform of [T7polymerase]Cr and [B0015]Ar (G2)


5/4 Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1) Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2) Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1) Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2) Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3) Transform of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2) Transform of [LacI] Ar (G3) Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2) Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1) Cultivation of [B0015] Ar, [PSB1A3] Ar(G1) Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)

5/5 Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1) Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3) Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1) Electrophoresis of digested product [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)

5/6 Mini-prep of [LacI] Ar (G3)

5/7 Transform of [PT7polymerase]Cr and [J61048]Ar (G2)

5/8 Transform of [B0015*3] Ar and [J61048*2] Ar (G2) Transform of [B0015] Ar and [J61048] Ar (G3) Transform of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1) Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2) Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2) Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)

5/9 Cultivation of [B0015] Ar, [J61048] Ar [LacI] Ar (G3)

5/10 Mini-prep [LacI] Ar (G3)


5/11 Cultivation of [J61048] Ar (G2) (ES) Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP) Transform of [T7 promoter] Kr (G2)

5/12 Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)

5/13 Transform of [T7 promoter] Kr (G2)

5/15 Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2) Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1) Mini-prep of [PSB1K3]Kr (G1) Digestion of [PSB1K3]Kr (EP) (G1)


5/19 Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)

5/20 Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2) Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1) Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)

5/21 Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2) Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1) Ligation of [K592100+J61048+PSB1K3] Kr (G1)

5/22 Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)

5/23 Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)

5/24 Digestion of [J61048] Ar (XP) (G2) PCR: [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2) Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)


5/27 Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2) Cultivation of [PSB1K3*3] Kr(G2)

5/28 PCR:[PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1) Mini-prep of [PSB1K3*3] Kr(G2) Digestion of [PSB1K3*3] Kr (EP) (G2) Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)

5/29 Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Transform of [J23101] Ar (G1) Transform of [T7 promoter] Ar Kr (G2) Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2) Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2) Ligation of [Plac+B0032+PSB1K3] Kr (G3)

5/30 Transform of ligation product (G2) Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3)


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