Team:NCTU Formosa/Notebook/test3

From 2014.igem.org

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<p>4/11
+
<p>6/2
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Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr  (G2)
+
PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
-
Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar  (G3)    
+
Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
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Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr  (G1)
+
Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)  
 +
</p>
<p>
<p>
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<p>4/12
+
6/3
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Cultivation of  [PSB1K3] Kr, [PSB1C3] Cr (G2)
+
PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5]  Kr (G2)
-
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
+
Electrophoresis of PCR products (G2)
-
Cultivation of [mGFP] Ar (G3)
+
Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
</p>
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<br></div>
 
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<div class="single note">
 
-
 
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<p>4/13
 
-
Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3)
 
-
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
 
-
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p>
 
-
 
-
<p>4/17
 
-
Transform of [PSB1K3] Kr (G1)</p>
 
-
 
-
<p>4/18
 
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Cultivation of [PSB1K3] Kr (G1) </p>
 
-
 
-
<p>4/19
 
-
Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3)
 
-
Mini-prep of [PSB1K3] Kr(G1) </p>
 
-
 
-
 
-
<br></div>
 
-
<div class="single note">
 
-
<p>4/20
 
-
Transform of [PSB1A3] Ar (G2)
 
-
Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p>
 
-
 
-
<p>4/21
 
-
Cultivation of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
 
-
 
-
<p>4/22
 
-
Mini-prep of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
 
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Revision as of 12:50, 9 October 2014

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6/2 PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)

6/3 PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of PCR products (G2) Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)