Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

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Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and
research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.  
research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.  
-
 
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<h3>Pneumosensor</h3>
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<div id="satu" align="center"> <h2>June 2013</h2>
 +
 
 +
      <div>
 +
      <a href="#" class="head">Week 1</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>June xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
      </div>
 +
 +
</div>
 +
</div>
 +
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<div id="satu" align="center"> <h2><center>July 2013</center></h2>
 +
 
 +
  <div>
 +
      <a href="#" class="head">Week 1</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br> <b> July 2 </b> <br>
 +
Wet lab <br>
 +
·      Digestion of pEGFP-N1 by Ase1 and BamH1 for PPAR-alpha promoter; , Xba1 and BamH1 for FABP1 promoter <br>
 +
·      LB-Chloramphenicol plates (the previous ones were found contaminated) <br>
 +
·      Inoculation of pEGFP-N1 for plasmid extraction <br>
 +
·      Transformation of P<i>fadBA</i> due chloramphenicol plates contamination <br>
 +
 +
<br> <b> July 3 </b> <br>
 +
·      Extraction of pEGFP-N1 plasmids by miniprep <br>
 +
·      PCR for PPAR-alpha promoter amplification <br>
 +
·      Digestion of pEGFP-N1 by Ase1 and BamH1 for PPAR-alpha promoter; , Xba1 and BamH1 for FABP1 promoter <br>
 +
 +
<br> <b> July 4</b> <br>
 +
Wet lab<br>
 +
·      Inoculation of BBa_K817002  P<i>fadBA</i><br>
 +
·      PCR for PPAR-alpha promoter and FABP1 promoter cloning from gDNA<br>
 +
·      Ran gel for digestion of pEGFP-N1 by Ase1 and BamH1 for PPAR-alpha promoter; Xba1 and BamH1 for FABP1 promoter; Ase1 and Xhol1 for GRP78<br>
 +
 +
<br> <b> July 5</b> <br>
 +
Wet lab<br>
 +
·      Extraction of BBa_K817002 (P<i>fadBA</i>) <br>
 +
·      Inoculation of pEGFP-N1<br>
 +
·      PCR for PPAR-alpha promoter and FABP1 promoter cloning from gDNA<br>
 +
·      Gel check, 0.8% gel for previously gDNA extraction<br>
 +
·      Ran gel for PCR check of PPAR-alpha promoter and FABP1 promoter<br>
 +
·      Poured new LB-Kanamycin plates<br>
 +
·      Transformation of <a href="http://parts.igem.org/Part:BBa_J176171">BBa_J176171</a><br>
 +
Dry lab<br>
 +
·      Discussion about re-design constructions, possible change for BBa_J176171 as mammalian expression vector<br>
 +
·      Primer redesign for PPAR-alpha promoter and FABP1 promoter<br><br>
 +
 +
 +
      </div>
 +
  </div>
 +
 
 +
  <div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br> <b> July 8</b> <br>
 +
Wet lab<br>
 +
·      gDNA extraction from HepG2 cells<br>
 +
·      Restriction of BBa_K817002 P<i>fadBA</i><br>
 +
·      Gel check for gDNA extraction<br>
 +
·      Inoculation of BBa_J176171, BBa_K817002 (P<i>fadBA</i>), pEGFP-N1 for plasmid extraction<br>
 +
·      New primers for PPAR-alpha promoter and FABP1 promoter arrival<br>
 +
·      Ran gel for BBa_K817002 P<i>fadBA</i> restriction check, gel extraction and purification<br>
 +
 +
<br> <b> July 9</b> <br>
 +
Wet lab<br>
 +
·      Extraction of BBa_J176171, BBa_K817002 P<i>fadBA</i> and pEGFP-N1 by miniprep<br>
 +
·      Restriction of pEGFP-N1 Restriction digestion by Ase1 and BamH1 for PPAR-alpha promoter; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1 promoter, Pst1 and Not1; followed by gel check, extraction and purification<br>
 +
·      Restriction of BBa_J176171 for P<i>fadBA</i> Ase1 and BamH1; FABP1 promoter by Xba1 and Not1<br>
 +
·      Ran gel for pEGFP-N1 and BBa_J176171 restriction products<br>
 +
·      PCR PPAR-alpha promoter and FABP1 promoter cloning from gDNA<br>
 +
·      <a href="http://parts.igem.org/Part:BBa_J52034">BBa_J52034</a> (<i>fad</i>R) transformation<br>
 +
 +
<br> <b> July 10</b> <br>
 +
Wet lab<br>
 +
·      Ran gel for PCR check of PPAR-alpha promoter and FAPB1 promoter<br>
 +
·      PCR for FABP1 promoter, PCR replication<br>
 +
·      BBa_J176171 vector dephosphorylation  by antartic phosphatase<br>
 +
·      Ligation of BBa_K817002 and P<i>fadBA</i> and BBa_J176171 <br>
 +
·      BBa_J52034 (<i>fad</i>R) inoculation<br>
 +
·      Digestion for BBa_K817002 P<i>fadBA</i> extraction<br>
 +
Gel check for BBa_K817002 P<i>fadBA</i> extraction<br>
 +
 +
<br> <b> July 11</b> <br>
 +
Wet lab<br>
 +
·      Gel purification for BBa_K817002 P<i>fadBA</i> extraction<br>
 +
·      Gel check for FABP1 promoter PCR product<br>
 +
·      PCR clean-up<br>
 +
·      BBa_J52034 (<i>fad</i>R) miniprep<br>
 +
·      BBa_J52034 restriction by Pst1 HF and Not1 HF<br>
 +
·      PCR  for PPAR-alpha promoter<br>
 +
Dry lab<br>
 +
Re-design constructions, now using BBa_J176171 as mammalian expression vector for all related constructions<br>
 +
 +
<br> <b> July 12</b> <br>
 +
Wet lab<br>
 +
·  Digestion of FABP1 promoter PCR product<br>
 +
·      Ran gel for PCR check of PPAR-alpha promoter<br>
 +
·      Digest pEGFP-n1 for BBa_J52034 <i>fad</i>R<br>
 +
·      Ran gel for pEGFP-n1 for BBa_J52034 <i>fad</i>R followed by gel purification<br>
 +
·      BBa_J176171 vector dephosphorylation by Antarctic phosphatase for FABP1 promoter and P<i>fadBA</i><br>
 +
Dry Lab<br>
 +
Primers design for pCMV cloning for <i>fad</i>R expression<br><br>
 +
      </div>
 +
  </div>
 +
 +
  <div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>July 15</b> <br>
 +
Wet lab<br>
 +
·      Ligation of FABP1 promoter, EGFP, and BBa_J176171, using 3 pieces ligation<br>
 +
·      Transformation of  FABP1 promoter, EGFP, and BBa_J176171 ligation<br>
 +
·      FABP1 promoter+EGFP +BBa_J176171 ligation restriction check<br>
 +
 +
<br><b>July 16</b> <br>
 +
Wet Lab<br>
 +
·      Miniprep for full construct of FABP1 promoter and pEGFP-N1<br>
 +
·      <i>fad</i>R and pEGFP-N1 Backbone parts ligation <br>
 +
·      Plasmids extraction for pCMV cloning for <i>fad</i>R<br>
 +
·      BBa_K817002 P<i>fadBA</i> promoter extraction by EcoR1 and Pst1 HF<br>
 +
·      BBa_J52034 restriction by EcoR1 and Pst1 HF<br>
 +
·      Inoculations for full construct of FABP1 promoter and pEGFP-N1<br>
 +
·      Streak colonies containing the right construct for FABP1 promoter<br>
 +
 +
<br><b>July 17</b> <br>
 +
Wet lab<br>
 +
·      Plasmid extraction for FABP1 promoter+EGFP+BBa_J176171 by miniprep<br>
 +
·      Repeat <i>fad</i>R and P<i>fadBA</i> constructs<br>
 +
·      Digest pEGFP-n1 and BBa-J176171 for <i>fad</i>R<br>
 +
·      Digestion for BBa_K817002 (P<i>fadBA</i>) extraction<br>
 +
·      Gel check and gel extraction for BBa_J176171 for <i>fad</i>R and BBa_K817002 (P<i>fadBA</i>) <br>
 +
 +
<br><b>July 18</b> <br>
 +
Wet lab<br>
 +
·      Gel purification for all digested products<br>
 +
·      FABP1 promoter digestion check for whole construct prior transfection<br>
 +
·      P<i>fadBA</i> vector de phosphorylation and ligation with EGFP and BBa_J176171<br>
 +
·      Transformation of P<i>fadBA</i>+EGFP+BBa_J176171<br>
 +
 +
<br><b>July 19</b> <br>
 +
Wet lab<br>
 +
·      FABP1 promoter preparation for transfection<br>
 +
·      Ligation of P<i>fadBA</i> and BBa_J176171 followed by transformation<br>
 +
·      PCR for PPAR-alpha promoter cloning<br>
 +
Dry lab<br>
 +
·      Design for characterization of the transfected cells with FABP1 construct<br>
 +
·      Review for Multiple Sites Mutagenesis<br>
 +
·      Ordered primers for pCMV cloning from pEGFP-N1<br><br>
 +
 +
      </div>
 +
  </div>
 +
 +
  <div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>July 22</b> <br>
 +
Wet lab<br>
 +
·  FABP1 construct given for characterization and transfection<br>
 +
·  Further colony screening for FABP1 construct, inoculations<br>
 +
·  Inoculation of P<i>fadBA</i> and BBa_J176171<br>
 +
·  Ran gel for PPAR-alpha promoter PCR products<br>
 +
 +
<br><b>July 23</b> <br>
 +
Wet lab<br>
 +
·  Primers arrival for pCMV cloning<br>
 +
·  PCR for pCMV cloning<br>
 +
·  Digestion and gel for construction check for FABP1 promoter<br>
 +
·  P<i>fadBA</i> gel extraction and purification, followed by vector dephosphorylation BBa_J176171 and ligation, then transformation<br>
 +
·  Digestion of pEGFP-N1 for <i>fad</i>R, followed by gel check and extraction<br>
 +
 +
<br><b>July 24</b> <br>
 +
Wet lab<br>
 +
·      DNA purification from gel extraction for digested pEGFP-N1<br>
 +
·      Multiple sites mutagenesis for illegal restriction sites for FABP1 promoter<br>
 +
·      Ran gel for pCMV cloning from pEGFP-N1<br>
 +
·      PCR clean up for pCMV cloning from pEGFP-N1<br>
 +
·      PCR for PPAR-alpha promoter with new primers using Taq polymerase<br>
 +
·      Transformation of mutagenesis products<br>
 +
·      Inoculation of P<i>fadBA</i> ligation colonies<br>
 +
 +
<br><b>July 25</b> <br>
 +
Wet lab<br>
 +
·      Ran gel check PPAR-alpha promoter PCR cloning with new primers using Taq polymerase<br>
 +
·      Minprep of P<i>fadBA</i>+EGFP+BBa_J176171 ligation colonies, followed by digestion check <br>
 +
 +
<br><b>July 26</b> <br>
 +
Wet lab<br>
 +
·      Multiple sites mutagenesis for illegal restriction sites for FABP1 promoter<br>
 +
·      Ran gel before parental string digestion of mutagenesis products<br>
 +
·      Digestion of pEGFP-N1 by Apa1 and Xba1 followed by gel check<br>
 +
·      Plasmids preparation according to transfection requirements, construct and controls<br><br>
 +
 +
      </div>
 +
  </div>
 +
 +
  <div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>July 30</b> <br>
 +
Wet lab<br>
 +
·      PPAR-alpha promoter PCR cloning with new primers using Vent polymerase<br>
 +
·      Restriction check for pEGFP-N1, using EcoR1, Xba1, Spe1 and Pst1<br>
 +
·      BBa_J176171 vector de phosphorylation, followed by ligation with EGFP using T4 Ligase, then transformation into E. Coli strain DH10b<br>
 +
·      PCR for PPAR-alpha promoter with new primers using Vent polymerase<br>
 +
Dry lab<br>
 +
·      Discussion and re assessment of constructs related to pEGFP-N1<br>
 +
 +
 +
<br><b>July 31</b> <br>
 +
·      Ran gel check for PPAR-alpha promoter PCR cloning with new primers using Vent polymerase<br>
 +
·      PCR for pCMV cloning from pEGFP-N1, followed by gel check and PCR clean up<br>
 +
·      PCR for PPAR-alpha promoter with reference primers using Vent polymerase<br>
 +
·      Ran gel check for PPAR-alpha promoter PCR cloning with reference primers using Vent polymerase<br><br>
 +
 +
      </div>
 +
  </div>
 +
</div>
 +
<!------      AAAAAAAAAAAA    U        U  GGGGGGGGGGGG    U          U    SSSSSSSSSSSSS    TTTTTTTTTTTTTT      2 0 1 3---------------->
 +
<!------      A          A    U        U  G              U          U    S                      T      2 0 1 3---------------->
 +
<!------      A          A    U        U  G              U          U    S                  T      2 0 1 3---------------->
 +
<!------      A      A   U        U  G              U          U    S                  T        2 0 1 3---------------->
 +
<!------      A      A   U     U  G              U          U    S                  T        2 0 1 3---------------->
 +
<!------      AAAAAAAAAAAA   U        U  G              U          U    SSSSSSSSSSSSS          T        2 0 1 3---------------->
 +
<!------      A        A   U        U  G    GGGGGGG    U          U                S          T        2 0 1 3---------------->
 +
<!------      A        A   U        U G      G  G    U          U                S          T        2 0 1 3---------------->
 +
<!------      A        A   U        U G      G  G    U          U                S          T 2 0 1 3---------------->
 +
<!------      A        A   U        U G          G    U          U                S        T 2 0 1 3---------------->
 +
<!------      A        A   UUUUUUUUUUU  GGGGGGGGGGGG    UUUUUUUUUUUUU    SSSSSSSSSSSSS        T        2 0 1 3---------------->
 +
 +
 +
<div id="satu" align="center"> <h2>August 2013</h2>
 +
 
 +
<div>
 +
      <a href="#" class="head">Week 1</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Aug xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
 +
      </div>
 +
    </div>
 +
</div>
 +
<!---- SSSSSSSS  EEEEEEEE  PPPPPPPP  TTTTTTTTTTTT  EEEEEEEE  M        M  BBBBBBBB    EEEEEEEEE  RRRRRRRR    2 0 1 3-------->
 +
<!---- S          E          P      P      T        E          MM      MM  B      B  E          R    R 2 0 1 3-------->
 +
<!---- S          E          P      P        T        E          M M    M M  B      B  E          R    R 2 0 1 3-------->
 +
<!---- S          E          P      P        T        E          M  M  M  M  B      B  E          R  R 2 0 1 3-------->
 +
<!----     S          E          P      P        T        E          M  M M  M  B      B  E          R    R 2 0 1 3-------->
 +
<!----      SSSSSSSS  EEEEEEEE  PPPPPPPP        T        EEEEEEEE  M    M    M  BBBBBBBB    EEEEEEEEE  RRRRRRRR 2 0 1 3-------->
 +
<!----            S  E          P              T        E          M        M  B      B  E          R  R      2 0 1 3-------->
 +
<!----   S  E          P              T        E          M        M  B      B  E          R    R  2 0 1 3-------->
 +
<!----   S  E          P              T        E          M        M  B      B  E          R    R 2 0 1 3-------->
 +
<!----   S  E          p              T        E          M        M  B      B  E          R      R 2 0 1 3-------->
 +
<!---- SSSSSSSS  EEEEEEEE  P              T        EEEEEEEE  M        M  BBBBBBBB    EEEEEEEEE  R  R 2 0 1 3-------->
 +
 +
<div id="satu" align="center"> <h2>September 2013</h2>
 +
 
 +
<div>
 +
      <a href="#" class="head">Week 1</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>August 2</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 2</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 3</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 4</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
<div>
 +
      <a href="#" class="head">Week 5</a>
 +
      <div class="content" align="left" style="display: none;">
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
<br><b>Sept xx</b> <br>
 +
Wet lab<br>
 +
·      Restriction check for EGFP+ BBa_J176171 using EcoR1 and Pst1HF<br>
 +
·      Consensus Kozak sequence extraction from BBa_J176171 by EcoR1 and Xba1, followed by gel check and DNA purification<br>
 +
·      PCR for PPAR-alpha promoter, using temperature gradient and every available set of primers designed, using Vent polymerase<br>
 +
·      Genomic DNA extraction from HepG2 cells, Phenol/Chloroform protocol<br><br>
 +
 +
 +
      </div>
 +
    </div>
 +
</div>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<h3>Riboregulator</h3>
 +
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Revision as of 14:14, 2 October 2014

Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






Pneumosensor

June 2013

Week 1
Week 2
Week 3
Week 4
Week 5

July 2013

Week 1
Week 2
Week 3
Week 4
Week 5

August 2013

Week 1
Week 2
Week 3
Week 4
Week 5

September 2013

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

June 2013

Week 1
Week 2
Week 3
Week 4
Week 5

July 2013

Week 1
Week 2
Week 3
Week 4
Week 5

August 2013

Week 1
Week 2
Week 3
Week 4
Week 5

September 2013

Week 1
Week 2
Week 3
Week 4
Week 5

Home

Team

Main Project

Side Project

Human Practice

Wetlab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook"