Team:Hong Kong HKUST/wetlab

From 2014.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Hong_Kong_HKUST/shell |
<html>
<html>
<head>
<head>
-
<link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/indexpage.css&action=raw&ctype=text/css" type="text/css" >
 
-
<script src="http://ajax.aspnetcdn.com/ajax/jQuery/jquery-1.11.1.min.js"></script>
 
-
<script>
 
-
$(document).ready(function(){
 
-
  $(window).scroll(function(){
 
-
var scrollTop = $(window).scrollTop();
 
-
if (scrollTop > 49) {
 
-
$('#menu').addClass('fixed');
 
-
} else {
 
-
$('#menu').removeClass('fixed');
 
-
}
 
-
  });
 
-
});
 
-
 
-
</script>
 
-
<script>
+
<style type="text/css">
-
var on = true;
+
-
var myVar = setInterval(function(){ if(on) {slides()}on=true;},3000);
+
#quick_link_wetlab {
-
 
+
font-size:50px;
-
function slides(i) {
+
text-align:center;
-
  document.getElementsByClassName("slide_pic")[1].style.width="0px";
+
align:center;
-
  document.getElementsByClassName("slide_pic")[2].style.width="100%";
+
}
-
  var slide = document.getElementsByClassName("slide_pic")[0];
+
-
  document.getElementById("slide").appendChild(slide);
+
</style>
-
  if(i) {
+
-
  on = false;  
+
-
  }
+
-
}
+
-
function back_slide(i) {
+
-
  document.getElementsByClassName("slide_pic")[1].style.width="0px";
+
-
  document.getElementsByClassName("slide_pic")[0].style.width="100%";
+
-
  var slide = document.getElementsByClassName("slide_pic")[4];
+
-
  var slide2 = document.getElementsByClassName("slide_pic")[0];
+
-
  document.getElementById("slide").insertBefore(slide,slide2);
+
-
  if(i) {
+
-
  on = false;
+
-
  }
+
-
}
+
-
</script>
+
</head>
</head>
 +
</html>|
 +
<html>
<body>
<body>
-
<body>
 
-
<div id='nav_container'>
 
-
<nav>
 
-
<ul id="menu">
 
-
<li><a href="#">Home</a></li>
 
-
        <li><a href="#">Team</a>
 
-
        <!-- First Tier Drop Down -->
 
-
        <ul>
 
-
            <li><a href="#">Members</a></li>
 
-
            <li><a href="#">Advisers</a></li>
 
-
            <li><a href="#">Instructor</a></li>
 
-
        </ul>       
 
-
        </li>
 
-
        <li><a href="#">Project</a>
 
-
        <!-- First Tier Drop Down -->
 
-
        <ul>
 
-
            <li><a href="#">Main Project</a>
 
-
<ul>
 
-
                <li><a href="#">Overview</a></li>
 
-
                <li><a href="#">Module one </a></li>
 
-
                <li><a href="#">Module two</a></li>
 
-
<li><a href="#">Data</a></li>
 
-
<li><a href="#">Characterization</a></li>
 
-
            </ul></li>
 
-
            <li><a href="#">Side Project</a>
 
-
<ul>
 
-
                <li><a href="#">Overview</a></li>
 
-
                <li><a href="#">Parts</a></li>
 
-
                <li><a href="#">Catalogue</a></li>
 
-
<li><a href="#">Feature pages</a></li>
 
-
            </ul></li>
 
-
       
 
-
        </ul>
 
-
        </li>
 
-
        <li><a href="#">Human Practice</a>
 
-
<ul>
 
-
                <li><a href="#">Overview</a></li>
 
-
                <li><a href="#">Start up kit</a>
 
-
<ul>
 
-
<li><a href="#">Interview</a></li>
 
-
<li><a href="#">Report</a></li>
 
-
<li><a href="#">Database</a></li>
 
-
</ul></li>
 
-
                <li><a href="#">Talks</a></li>
 
-
<li><a href="#">Outreach</a></li>
 
-
            </ul></li>
 
-
        <li><a href="#">wetlab</a>
 
-
<ul>
 
-
<li><a href="#">Protocol</a></li>
 
-
<li><a href="#">Notebook</a></li>
 
-
<li><a href="#">Safety</a></li>
 
-
</ul></li>
 
-
        <li><a href="#">Achievement</a>
 
-
<ul>
 
-
<li><a href="#">Medal Requirement</a></li>
 
-
<li><a href="#">Deliverable</a></li>
 
-
</ul></li>
 
-
<li class= "logo"><a href="https://2014.igem.org/Main_Page"> <img src="Igemlogo_whiteletters.png" height="45px"> </a> </li>
 
-
<li class= "logo"><a href="http://www.ust.hk/"> <img src="HKUST_logo.png" height="45px"> </a></li>
 
-
 
-
</ul>
 
-
 
-
</nav>
 
-
</div>
 
-
<h1> </h1>
 
-
<!-- ================ do not touch any thing above this, dont even think about it =========================-->
 
-
 
<div id="content_container">
<div id="content_container">
-
<div class= "banner_area">
+
<div class='content_1'>
-
<img src= 'banner_mod.png' />
+
<h3 style="text-align:center">Wetlab</h3>
-
<!--banner should be larger and height should be more than 400px-->
+
<table class="content_table" align= "center" valign= "top">
-
</div>
+
<tr class= "content_row">
-
<hr>
+
<td class= "content_cell">
-
<div id="description_area">
+
<div class= "content_area_one_row">
-
<h2>Protocol</h2>
+
<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
-
<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
+
(1) (Detection Module) ComD & ComE, (2) (Detection Module) P<sub><i>comCDE</i></sub>, (3) (&sigma;<sup>X</sup> promoters Module) &sigma;<sup>X</sup> & ComW, and
-
  can be found here!
+
(4) (&sigma;<sup>X</sup> promoters Module) P<sub>celA</sub> and P<sub>comFA</sub>. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
 +
and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
 +
The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process,
 +
some experiments could not be completed due to time constrains and they are outlined in Future Work page.
</p>
</p>
 +
</div>
 +
</td>
 +
</tr>
 +
</table>
-
</div>
 
<div id= 'project_buttons'>
<div id= 'project_buttons'>
<table id = "project_buttons_table" align= "center" >
<table id = "project_buttons_table" align= "center" >
-
<tr class= "start_up_link_row">
+
<tr class= "start_up_link_row" align="center">
-
+
-
<td class= "start_up_link_button">
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
+
-
<h4> Mutagenesis</h4>
+
-
<p> Protocol description/picture here. </p>
+
-
</div></a>
+
-
</td>
+
-
+
<td class= "start_up_link_button">
<td class= "start_up_link_button">
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">  
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook"><div class= "start_up_link_container">  
-
<h4> Western Blot </h4>
+
<div id="quick_link_wetlab">
-
<p> Protocol description/picture here. </p>
+
Notebook
 +
<hr>
 +
<img src="https://static.igem.org/mediawiki/2014/2/28/NotebookHKUST.png" width="200" height="200">
 +
</div>
</div>
</div>
</td>
</td>
<td class= "start_up_link_button">
<td class= "start_up_link_button">
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"><div class= "start_up_link_container">  
-
<h4> LB agar and LB plates </h4>
+
<div id="quick_link_wetlab">
-
<p> Protocol description/picture here. </p>
+
Protocols
 +
<hr>
 +
<img src="https://static.igem.org/mediawiki/2014/2/26/HKUST_Quicklink_Icon_Protocols.png" width="200" height="200">
 +
</div>
 +
</div>
</div>
</td>
</td>
-
<td class= "start_up_link_button">
 
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
 
-
<h4> Ligation </h4>
 
-
<p> Protocol description/picture here. </p>
 
-
</div>
 
-
</td>
 
-
</tr>
 
-
<tr class= "start_up_link_row">
 
<td class= "start_up_link_button">
<td class= "start_up_link_button">
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">  
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety"><div class= "start_up_link_container">
-
<h4> Ethanol Precipitation</h4>
+
<div id="quick_link_wetlab">
-
<p> Protocol description/picture here. </p>
+
Safety
-
</div>
+
<hr>
-
</td>
+
<img src="https://static.igem.org/mediawiki/2014/e/e1/HKUST_Quicklink_Icon_Safety.png" width="200" height="200">
-
+
</div>
-
<td class= "start_up_link_button">
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
+
-
<h4> Pbad protocols </h4>
+
-
<p>Protocol description/picture here. </p>
+
-
</div>
+
-
</td>
+
-
<td class= "start_up_link_button">
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">
+
-
<h4> Gibson Assembly </h4>
+
-
<p> Protocol description/picture here. </p>
+
-
</div>
+
-
</td>
+
-
<td class= "start_up_link_button">
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/testPDF"><div class= "start_up_link_container">  
+
-
<h4> Polymerase Chain Reaction (PCR)</h4>
+
-
<p> Protocol description/picture here. </p>
+
</div>
</div>
</td>
</td>
</tr>
</tr>
 +
 +
</table>
</table>
</div>
</div>
 +
<hr>
<hr>
-
+
</div>
-
<table class= "site_map_table" align= "center">
+
</div>
-
<tr>
+
-
<td class= 'site_map_column'><a href="#"><h4>Home</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Team</li></a>
+
-
<li class='site_link'><a href="#">Project</li></a>
+
-
<li class='site_link'><a href="#">WetLab</li></a>
+
-
<li class='site_link'><a href="#">Achievement</li></a>
+
-
<li class='site_link'><a href="#">Human Practice</li></a>
+
-
<li class='site_link'><a href="#">Side Project</li></a>
+
-
</ul>
+
-
 
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Team</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Member</li></a>
+
-
<li class='site_link'><a href="#">Advisor</li></a>
+
-
<li class='site_link'><a href="#">Instructor</li></a>
+
-
<li class='site_link'><a href="#">Attribution</li></a>
+
-
<li class='site_link'><a href="#">Acknowledgement</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Main Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Module one</li></a>
+
-
<li class='site_link'><a href="#">Module two</li></a>
+
-
<li class='site_link'><a href="#">Data</li></a>
+
-
<li class='site_link'><a href="#">Characterisation</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Side Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Parts</li></a>
+
-
<li class='site_link'><a href="#">Catalogue</li></a>
+
-
<li class='site_link'><a href="#">Feature Pages</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Human Practice</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Start up kit</li></a>
+
-
<ul>
+
-
<li class='site_link'><a href="#">Interview</li></a>
+
-
<li class='site_link'><a href="#">Report</li></a>
+
-
<li class='site_link'><a href="#">Database</li></a>
+
-
</ul>
+
-
<li class='site_link'><a href="#">Talks</li></a>
+
-
<li class='site_link'><a href="#">Outreach</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Wetlab</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Protocol</li></a>
+
-
<li class='site_link'><a href="#">Notebook</li></a>
+
-
<li class='site_link'><a href="#">Safety</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Achievement</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Medal Requirements</li></a>
+
-
<li class='site_link'><a href="#">Deliverable</li></a>
+
-
</ul>
+
-
</td>
+
-
</tr>
+
-
 
+
-
</table>
+
</body>
</body>
-
</html>
+
</html>  
 +
}}

Latest revision as of 22:01, 17 October 2014



Wetlab

The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) (Detection Module) ComD & ComE, (2) (Detection Module) PcomCDE, (3) (σX promoters Module) σX & ComW, and (4) (σX promoters Module) PcelA and PcomFA. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.


Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement