Team:Hong Kong HKUST/pneumosensor


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Pneumosensor Project Abstract

Figure 1. Pneumosensor Overview Diagram

Quorem-sensing peptide CSP produced by S. pneumoniae is detected by the Detection Module; the response regulator ComE links the Detection Module to the σx Promoters Module which ultimately drives the expression of GFP.

Streptococcus pneumoniae is responsible for approximately 1.6 million deaths every year mostly in developing countries where vaccination is unaffordable. To provide an affordable solution to combat pneumococcal diseases, we are engineering an E. coli "Pneumosensor", that will work inside as an in vitro diagnostic device. Through this cheaper and easier diagnosis method, people can prepare themselves better against the transmission and adversity of S. pneumoniae.

Pneumosensor has a rewired comCDE signal transduction pathway native to S. pneumoniae that detects autoinducer molecules released specifically by S. pneumoniae. It is also equipped with a new promoter for the specific and tight regulation of target gene expression in E. coli.




Human Practice