Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay

From 2014.igem.org

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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)                 each of 2.5&micro;L to DH5&alpha;
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Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)  
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</div>
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<div class="step-recipe">
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each of 2.5&micro;L to DH5&alpha;
</div>
</div>
    </div>
    </div>
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      <div class="step-recipe">
      <div class="step-recipe">
Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1)
Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1)
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</div>
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<div class="step-recipe">
LB 2 mL
LB 2 mL
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</div>
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<div class="step-recipe">
for 3hours
for 3hours
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</div>
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<div class="step-recipe">
Turbidity was controlled 0.1
Turbidity was controlled 0.1
</div>
</div>
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      <div class="step-recipe">
      <div class="step-recipe">
LB 2 mL
LB 2 mL
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</div>
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<div class="step-recipe">
IPTG 20 &micro; or not
IPTG 20 &micro; or not
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</div>
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<div class="step-recipe">
for 11 hours, 13hours, 15hours, 17 hours
for 11 hours, 13hours, 15hours, 17 hours
</div>
</div>

Revision as of 02:49, 18 October 2014

Notebook
Lab Documents

140910 asB0034&B0034 mRFP Assay

  • Start

  • Double Transformation

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)
    each of 2.5µL to DH5α
  • Colony PCR

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃
  • Preculture

    Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1)
    LB 2 mL
    for 3hours
    Turbidity was controlled 0.1
  • Culture

    LB 2 mL
    IPTG 20 µ or not
    for 11 hours, 13hours, 15hours, 17 hours
  • Assay

    fluoresence imageing 514 nm 610 filter
  • Complete!