Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

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Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • mini-Prep

    1A3 Vector
  • PCR

    Plac-stem-as-stem-dT on 1C3 with 100up_F & 200dn_R
  • Digestion

    1A3 with EcoRI & PstI, Plac-stem-as-stem-dT with EcoRI & PstI
  • Ligation

    1A3 & Plac-stem-as-stem-dT
  • Transformation

    Plac-stem-as-stem-dT on 1A3
  • Complete!