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Team:Evry/Notebook/Sensing/PCBs/week6
From 2014.igem.org
(Difference between revisions)
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<u> <h3> Sensor construction hbpR-hbpC </h3> </u> | <u> <h3> Sensor construction hbpR-hbpC </h3> </u> | ||
| + | <h4> 08/4/14 :</h4> | ||
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| + | <h4> 08/5/14 :</h4> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/8/81/Primers11082014.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/8/81/Primers11082014.jpg" width="400px;" class="thumbimage"/> | ||
| + | </a> | ||
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| + | <div class="magnify"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/81/Primers11082014.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>Table 1: Received primers to check part BBa_K1155001 (Part given by Paris-Saclay team) </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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<h4> 08/6/14 :</h4> | <h4> 08/6/14 :</h4> | ||
<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | <u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | ||
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C | <br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C | ||
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<h4> 08/7/14 :</h4> | <h4> 08/7/14 :</h4> | ||
<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | <u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | ||
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm | <br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm | ||
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<h4> 08/8/14 :</h4> | <h4> 08/8/14 :</h4> | ||
<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | <u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u> | ||
<br/> Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL | <br/> Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL | ||
</p> | </p> | ||
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| + | <h4> 08/9/14 :</h4> | ||
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| + | <h4> 08/10/14 :</h4> | ||
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</div> | </div> | ||
Revision as of 15:57, 19 August 2014
Week 6
Sensor construction hbpR-hbpC
08/4/14 :
08/5/14 :
08/6/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
08/7/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
08/8/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL
