Team:Cornell/project/drylab/testing

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<h1 style="margin-top: 0px;">Header 1</h1>
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<h1>CT Scan</h1>
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Text text text Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt.  
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To get a closer look at the internal structure of our hollow fiber reactor and to monitor the durability of the fibers after heavy filtration we got a Computed Tomography (CT) scan of the reactor with the help of Cornell's Biotechnology Resource Center Imaging Center. We are very concerned with how the fiber material holds up to use as we don't want the filter to be compromised and for our cells to escape into the environment. This technique could be used to determine the life-span and suggested replacement times of such systems.
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
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<h1>Material Design</h1>
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The box has been tested to last for over 5 months in our project for 2012.  Since it is meant to be used outside in the field, the box must be durable and water proof in order to filter the polluted water and safeguard the electronics from the environment.  The box is also self-sustaining in a sense that a solar panel is used to provide electricity for the electronics and that it can endure the environment as it may be placed by a river to sequester some of the heavy metals from the river.
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
 
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But I must explain to you how all this mistaken idea of denouncing pleasure and praising pain was born and I will give you a complete account of the system, and expound the actual teachings of the great explorer of the truth, the master-builder of human happiness.
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<h1>Flow Rate Testing</h1>
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In order to determine our requirements for the pump, we tested the flow rate through a setup with the prefilter and fiber reactor. Since the fiber reactor membranes are extremely delicate, we had to make sure our pump could overcome the system’s high resistance without causing any leaks. Initially, this was quite difficult, since we had to prime the system with water for the pump to work at all. The prefilter does not outlet any water until it is completely full. We were able to solve this by connecting the inlet to a separate water source and using hydrostatic head to carefully fill each component. We then matched the fiber reactor’s maximum pressure rating to the pump’s outlet pressure based on the current from the power supply.  
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<h1>Header 2</h1>
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, vex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<h1>Luria Broth Testing</h1>
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One factor in the longevity of living cells in our fiber reactor system is the retention time of growth media in the outer chamber. To test this retention time, a test was conducted to see how long it took for LB to completely leave the fiber reactor. Tonic water fluoresces under UV light because it contains quinine, so solutions of LB and tonic water at varying ratios were run through the fiber reactor. The volume and amount of dilution of the mixture leaving the fiber reactor was measured via the amount of fluorescence. Overall, it was determined that LB does not remain in the fiber reactor for a long enough time to support growth. To support long-term growth, we would have to incorporate a separate media cycling system into the reactor. However, this reactor system uses very slowly growing cells that does not require media for growth, so this is not a huge concern for our project.  
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No one rejects, dislikes, or avoids pleasure itself, because it is pleasure, but because those who do not know how to pursue pleasure rationally encounter consequences that are extremely painful. Nor again is there anyone who loves or pursues or desires to obtain pain of itself, because it is pain, but because occasionally circumstances occur in which toil and pain can procure him some great pleasure. To take a trivial example, which of us ever undertakes laborious physical exercise, except to obtain some advantage from it? But who has any right to find fault with a man who chooses to enjoy a pleasure that has no annoying consequences, or one who avoids a pain that produces no resultant pleasure? On the other hand, we denounce with righteous indignation and dislike men who are so beguiled and demoralized by the charms of pleasure of the moment, so blinded by desire, that they cannot foresee.
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The picture on the right, from left to right, is 50/50 LB-tonic water mixture inserted into the reactor, the output from the fiber reactor, the mixture left in the fiber reactor, and water.  
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<p>More detail. Stuff stuff stuff stuff.</p>
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<p><a href="#" class="btn btn-primary" role="button">Button</a> <a href="#" class="btn btn-default" role="button">Button</a></p>
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, ex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<h1>References</h1>
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Latest revision as of 23:32, 17 October 2014

Cornell iGEM

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Dry Lab

CT Scan

To get a closer look at the internal structure of our hollow fiber reactor and to monitor the durability of the fibers after heavy filtration we got a Computed Tomography (CT) scan of the reactor with the help of Cornell's Biotechnology Resource Center Imaging Center. We are very concerned with how the fiber material holds up to use as we don't want the filter to be compromised and for our cells to escape into the environment. This technique could be used to determine the life-span and suggested replacement times of such systems.

Material Design

The box has been tested to last for over 5 months in our project for 2012. Since it is meant to be used outside in the field, the box must be durable and water proof in order to filter the polluted water and safeguard the electronics from the environment. The box is also self-sustaining in a sense that a solar panel is used to provide electricity for the electronics and that it can endure the environment as it may be placed by a river to sequester some of the heavy metals from the river.

Flow Rate Testing

In order to determine our requirements for the pump, we tested the flow rate through a setup with the prefilter and fiber reactor. Since the fiber reactor membranes are extremely delicate, we had to make sure our pump could overcome the system’s high resistance without causing any leaks. Initially, this was quite difficult, since we had to prime the system with water for the pump to work at all. The prefilter does not outlet any water until it is completely full. We were able to solve this by connecting the inlet to a separate water source and using hydrostatic head to carefully fill each component. We then matched the fiber reactor’s maximum pressure rating to the pump’s outlet pressure based on the current from the power supply.

Luria Broth Testing

One factor in the longevity of living cells in our fiber reactor system is the retention time of growth media in the outer chamber. To test this retention time, a test was conducted to see how long it took for LB to completely leave the fiber reactor. Tonic water fluoresces under UV light because it contains quinine, so solutions of LB and tonic water at varying ratios were run through the fiber reactor. The volume and amount of dilution of the mixture leaving the fiber reactor was measured via the amount of fluorescence. Overall, it was determined that LB does not remain in the fiber reactor for a long enough time to support growth. To support long-term growth, we would have to incorporate a separate media cycling system into the reactor. However, this reactor system uses very slowly growing cells that does not require media for growth, so this is not a huge concern for our project.

The picture on the right, from left to right, is 50/50 LB-tonic water mixture inserted into the reactor, the output from the fiber reactor, the mixture left in the fiber reactor, and water.

Retrieved from "http://2014.igem.org/Team:Cornell/project/drylab/testing"