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Team:CityU HK/notebook/lablog
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Revision as of 07:45, 17 September 2014
-
JulyOpen or Close
Week 3 (13/7~19/7)
- Primer design for leaderless tesA for Fit-coli (‘tesA) & New biobrick (‘tesA BB)
Week 4 (20/7~26/7)
- PCR of ‘tesA and ‘tesA BB
- PCR purification of ‘tesA and ‘tesA BB
- Prepare LB agar plates(‘tesA BB)
Week 5 (27/7~31/7)
- Digestion of ‘tesA BB and pSB1C3 plasmid with EcoRI & PstI restriction enzymes
- PCR purification for digested ‘tesA BB
- Sub-cloning of ‘tesA BB into pSB1C3 plasmid
- Transformation of ‘tesA BB into W3110 E.coli
- Pick colonies of the transformed E. coli with ‘tesA BB
-
JulyOpen or Close
Week 3 (13/7~19/7)
- Primer design for leaderless tesA for Fit-coli (‘tesA) & New biobrick (‘tesA BB)
Week 4 (20/7~26/7)
- PCR of ‘tesA and ‘tesA BB
- PCR purification of ‘tesA and ‘tesA BB
- Prepare LB agar plates(‘tesA BB)
Week 5 (27/7~31/7)
- Digestion of ‘tesA BB and pSB1C3 plasmid with EcoRI & PstI restriction enzymes
- PCR purification for digested ‘tesA BB
- Sub-cloning of ‘tesA BB into pSB1C3 plasmid
- Transformation of ‘tesA BB into W3110 E.coli
- Pick colonies of the transformed E. coli with ‘tesA BB
