Team:Caltech/week13

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Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week Thirteen

Sunday, 9/7/14

Monday, 9/8/14

lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Tuesday, 9/9/14

Export Systems
Preparation of fsr system for LC/MS analysis:
  • Made new LB-CARB plates, since the old stock had run out.
  • Transformed pTG005-unFLAG into DH5α. Cells were plated & incubated overnight.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Wednesday, 9/10/14

Export Systems
Preparation of fsr system for LC/MS analysis:
  • Set up a liquid culture of pTG005-unFLAG cells.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Thursday, 9/11/14

Export Systems
Preparation of fsr system for LC/MS analysis:
  • Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Friday, 9/12/14

Export Systems
Preparation of fsr system for LC/MS analysis:
  • aTc-induced MOPS liquid cultures from yesterday were spun down, and then the supernatant was loaded into C18 columns, washed with 10% acetonitrile, and then eluted with 60% acetonitrile.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Saturday, 9/13/14

Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters