Team:Bielefeld-CeBiTec/Results/DataPage

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<p>
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This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project">project part</a> of the Wiki.</p>  
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This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project">project part</a> of the Wiki.
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<br>
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With our project we like to address the problem of energy storage as well as the increasing atmospheric concentration of carbon dioxide. Excess electricity will be used to bind atmospheric carbon dioxide in bacteria. The carbon is used to produce different commercially interesting products. As a proof of concept we decided to implement this system using <i>Escherichia coli</i>. Major advantages of our concept are the use of carbon dioxide from the atmosphere under usage of available electricity on the one hand and the production of isobutanol on the other.
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<font size="2" style="text-align:left;"><b>Figure 1</b>: Project description</font>
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<h6> Data for rMFC</h6>
<h6> Data for rMFC</h6>
<table style="background-color:transparent">
<table style="background-color:transparent">
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<h6> Data for CO<sub>2</sub> Fixation</h6>
<h6> Data for CO<sub>2</sub> Fixation</h6>
<table style="background-color:transparent">
<table style="background-color:transparent">
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   <td><a href="http://parts.igem.org/Part:BBa_K1465212" target="_blank">BBa_K1465212</a></td><td>Phosphoribulokinase prkA from <i>Synechoccus elongatus</i> under the control of the ptac promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465212" target="_blank">BBa_K1465212</a></td><td>Phosphoribulokinase prkA from <i>Synechoccus elongatus</i> under the control of the ptac promoter</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465226" target="_blank">BBa_K1465226</a></td><td>Knock-down with sRNA of phosphofructo kinase under control of ptac promotor (ptac_sRNA:pfkA)</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465226" target="_blank">BBa_K1465226</a></td><td>Knock-down with sRNA of phosphofructo kinase under control of ptac promoter (ptac_sRNA:pfkA)</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465227" target="_blank">BBa_K1465227</a></td><td>Knock-down with sRNA of phosphofructo kinase under control of T7 promotor. (t7_sRNA:pfkA)</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465227" target="_blank">BBa_K1465227</a></td><td>Knock-down with sRNA of phosphofructo kinase under control of T7 promoter. (t7_sRNA:pfkA)</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465229" target="_blank">BBa_K1465229</a></td><td>Sedoheptulose 1,7-bisphosphatase (glpX) from <i>Bacillus methanolicus</i> under the control of the ptac promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465229" target="_blank">BBa_K1465229</a></td><td>Sedoheptulose 1,7-bisphosphatase (glpX) from <i>Bacillus methanolicus</i> under the control of the ptac promoter</td>
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<h6> Data for Isobutanol</h6>
<h6> Data for Isobutanol</h6>
<table style="background-color:transparent">
<table style="background-color:transparent">
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   <td><a href="http://parts.igem.org/Part:BBa_K1465301" target="_blank">BBa_K1465301</a></td><td>Alochol dehydrogenase from <i>Lactococcus lactis</i></td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465301" target="_blank">BBa_K1465301</a></td><td>Alcohol dehydrogenase from <i>Lactococcus lactis</i></td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465304" target="_blank">BBa_K1465304</a></td><td>Alcohol dehydrogenase under control of T7 promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465304" target="_blank">BBa_K1465304</a></td><td>Alcohol dehydrogenase under control of T7 promoter</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465305" target="_blank">BBa_K1465305</a></td><td>Alcohol dehydrogenase under control of ptac promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465305" target="_blank">BBa_K1465305</a></td><td>Alcohol dehydrogenase under control of ptac promoter</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465306" target="_blank">BBa_K1465306</a></td><td>Isobutanol producing pathway under control of ptac promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465306" target="_blank">BBa_K1465306</a></td><td>Isobutanol producing pathway under control of ptac promoter</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465307" target="_blank">BBa_K1465307</a></td><td>Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promotor</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465307" target="_blank">BBa_K1465307</a></td><td>Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promoter</td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465401" target="_blank">BBa_K1465401</a></td><td>Alanin racemase (alr) knock-out strain fused with RFP as visual control for transformation in <i>E.coli</i></td>
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   <td><a href="http://parts.igem.org/Part:BBa_K1465401" target="_blank">BBa_K1465401</a></td><td>Alanin racemase (alr) knock-out strain fused with RFP as visual control of transformation in <i>E.coli</i></td>
  </tr>
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Latest revision as of 03:42, 18 October 2014


Data Page

This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our project part of the Wiki.
With our project we like to address the problem of energy storage as well as the increasing atmospheric concentration of carbon dioxide. Excess electricity will be used to bind atmospheric carbon dioxide in bacteria. The carbon is used to produce different commercially interesting products. As a proof of concept we decided to implement this system using Escherichia coli. Major advantages of our concept are the use of carbon dioxide from the atmosphere under usage of available electricity on the one hand and the production of isobutanol on the other.

Figure 1: Project description
Data for rMFC
BioBrickDescription
BBa_K1465101Cytochrome c from Geobacter sulfurreducens for electron transfer with mediators for reduction equivalent generation
BBa_K1465102Fumerate reductase (frd) from E. coli under the control of the T7 promoter
BBa_K1465103Fumerate reductase (FumABCD) from Actinobacillus succinogenes
BBa_K1465104Fumarate reductase FumBCD from Actinobacillus succinogenes
BBa_K1465105Fumarate reductase (Fum) under control of the T7 promoter
BBa_K1465107Primer sequence for genome editing of porine fwd
BBa_K1465108Primer sequence for genome editing of porine rev
BBa_K1465109Primer sequence for genome editing of dcuB fwd
BBa_K1465110Primer sequence for genome editing of dcuB rev
Data for CO2 Fixation
BioBrickDescription
BBa_K1465201Phosphoribulokinase prkA from Synechoccus elongatus, codon optimized for E. coli.
BBa_K1465202Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus
BBa_K1465203Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechoccus elangatus.
BBa_K1465204Shell-associated protein (csoS2) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465205Carbonic anhydrase (csoS3) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465206shell proteins (csoS4) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465207pore-forming protein (csoS1D) of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465208can + shell proteins (cloning intermediate) from Halothiobacillus neapolitanus
BBa_K1465209Carbonic anhydrase + shell proteins + csoS1D from Halothiobacillus neapolitanus
BBa_K1465212Phosphoribulokinase prkA from Synechoccus elongatus under the control of the ptac promoter
BBa_K1465213RubisCO from Halothiobacillus neapolitanus under control of the ptac promoter
BBa_K1465214Phosphoribulokinase of Halothiobacillus neapolitanus under control of ptac
BBa_K1465222shell-protein fused with GFP
BBa_K1465223Empty Carboxysome with GFP
BBa_K1465225Knock-down with sRNA of phosphofructo kinase. (sRNA:pfkA)
BBa_K1465226Knock-down with sRNA of phosphofructo kinase under control of ptac promoter (ptac_sRNA:pfkA)
BBa_K1465227Knock-down with sRNA of phosphofructo kinase under control of T7 promoter. (t7_sRNA:pfkA)
BBa_K1465228Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus
BBa_K1465229Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus under the control of the ptac promoter
BBa_K1465230Purification vector (T7-RBS Intein-tag)
Data for Isobutanol
BioBrickDescription
BBa_K1465301Alcohol dehydrogenase from Lactococcus lactis
BBa_K1465302Isobutanol producing pathway (alsS_ilvC_ilvD_kivD)
BBa_K1465303Isobutanol producing pathway combined with alcohol dehydrogenase (alsS_ilvC_ilvD_kivD_adhA)
BBa_K1465304Alcohol dehydrogenase under control of T7 promoter
BBa_K1465305Alcohol dehydrogenase under control of ptac promoter
BBa_K1465306Isobutanol producing pathway under control of ptac promoter
BBa_K1465307Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promoter
Data for Biosafety
BioBrickDescription
BBa_K1465401Alanin racemase (alr) knock-out strain fused with RFP as visual control of transformation in E.coli
BBa_K1465403Primer for alanin racemase (alr) fwd
BBa_K1465404Primer for alanin racemase (alr) rev
BBa_K1465405Primer for dadX fwd
BBa_K1465406Primer for dadX rev

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