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Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
(Difference between revisions)
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<td width="30%">pSB1C3_suf_sRNApfkA (43 bp)</td> | <td width="30%">pSB1C3_suf_sRNApfkA (43 bp)</td> | ||
<td width="70%">5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
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Revision as of 12:40, 24 August 2014
Primerlist
| Primer ID | Primer sequence |
|---|---|
| Module 1 | |
| Gene synthesis | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| CCM-Cassette (E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
| Cytochrom C (Geobacter sulfurreducens) | |
| pccH_fwd (55 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘ |
| pccH_rev (40 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘ |
| pSB1C3_pre_pccH (58 bp) | 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_pccH (43 bp) | 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| ??? (Julian) | |
| T7_frd_Ec_fwd (64 bp) | 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘ |
| T7_frd_Ec_rev (44 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘ |
| pSB1C3_frd_pre (45 bp) | 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘ |
| pSB1C3_frd_suf (47 bp) | 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pR6K_dcuB_short (22 bp) | 5‘-AATTAACCCTCACTAAAGGGCG-3‘ |
| Module 2 | |
| Carbonic anhydrase | |
| csoS3_can_fwd (50 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘ |
| pSB1C3_pre_can (46 bp) | 5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘ |
| csoS3_can_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘ |
| pSB1C3_suf_can (46 bp) | 5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| Shell associated protein | |
| csoS2_sap_1_fwd (43) | 5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘ |
| pSB1C3_pre_sap_1 (43 bp) | 5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| csoS2_sap_1_rev (38 bp) | 5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘ |
| csoS1D | |
| csoS1D_fwd (40 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘ |
| csoS1D_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘ |
| pSB1C3_suf_csoS1D (43bp) | 5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_csoS1D (43 bp) | 5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘ |
| Second part | |
| fw_pSB1C3_csoS4A (47 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘ |
| rv_csoS4A_PstI (43 bp) | 5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘ |
| fw_PstI_csoS4A (51 bp) | 5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘ |
| rv_SpeI_Intergen (52 bp) | 5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘ |
| fw_SpeI_Intergen (49 bp) | 5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘ |
| rv-csoS1-pSB1C3 (50 bp) | 5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘ |
| fw-csoS1-pSB1C3 (46 bp) | 5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘ |
| rv-pSB1C3-csoS4A (47 bp) | 5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| Gluco switch | |
| pSB1C3_pre_sRNApfkA (43 bp) | 5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_sRNApfkA (43 bp) | 5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| General primer | |
| int_RBS_fw (1) (42 bp) | 5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘ |
| RBS_int_rev (2) (44 bp) | 5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘ |
| RBS_rev (3) (22 bp) | 5‘-TTTCTCCTCTTTCCCTATAGTG-3‘ |
| int_fw (4) (22 bp) | 5‘-TGCATCACGGGAGATGCACTAG-3‘ |
| int_pSB1C3_rev (5) (50 bp) | 5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘ |
| pSB1C3_int_fw (6) (46 bp) | 5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| SBPase (glpX) | |
| SBPase_purify_fw (45 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘ |
| SBPase_purify_rev (53 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘ |
| fw_pSB1_SBPase (58 bp) | 5‘-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3‘ |
| rv_pSB1_SBPase (56 bp) | 5‘-GACTGCAGCGGCCGCTACTAGTATCAATGATAAGCTTCAATAAATTGGTATTTTAC-3‘ |
| fw_SBPase_XbaI (56 bp) | 5‘-AAAAGCAAAAGGCTGCGTAAATTTGGACGCATCTTTAACAGAAAATATGAAATCAG-3‘ |
| rv_SBPase_XbaI (46 bp) | 5‘-TTTTCTGTTAAAGATGCGTCCAAATTTACGCAGCCTTTTGCTTTTG-3‘ |
| fw_SBPase_pSB1C3 (57 bp) | 5‘-AATTTATTGAAGCTTATCATTGATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG-3‘ |
| rv_SBPase_pSB1 (65 bp) | 5‘-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3‘ |
| fbaA | |
| fbaA_purify_fw (50 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘ |
| fbaA_purify_rev (41 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘ |
| fbaA_PstI_fw (42 bp) | 5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘ |
| fbaA_PstI_rev (43 bp) | 5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘ |
| tktA | |
| tktA_purify_fw (43 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘ |
| tktA_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘ |
| tktA_PstI_fw (41 bp) | 5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘ |
| tktA_PstI_rev (42 bp) | 5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘ |
| cg_pgk | |
| cg_pgk_purify_fw (42 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘ |
| cg_pgk_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘ |
| gapA | |
| gapA_purify_fw (47 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘ |
| gapA_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘ |
| gapA_NotI_fw (44 bp) | 5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘ |
| gapA_NotI_rev (43 bp) | 5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘ |
| prkA | |
| prkA_purify_fw (40 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘ |
| prkA_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘ |
| pykF | |
| pykF_purify_fw (47 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘ |
| pykF_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘ |
| pykF_PstI_fw (48 bp) | 5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘ |
| pykF_PstI_rev (43 bp) | 5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘ |
| ldhA | |
| ldhA_purify_fw (44 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘ |
| ldhA_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘ |
| glk | |
| glk_purify_fw (44 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘ |
| glk_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘ |
| zwf | |
| zwf_purify_fw (41 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘ |
| zwf_purify_rev (45 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘ |
| Gene synthesis | |
| pSB1C3_pre_rbcLS (44 bp) | 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_rbcLS (43 bp) | 5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccbLS (44 bp) | 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_ccbLS (43 bp) | 5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_prkA (43 bp) | 5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_prkA (43 bp) | 5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| Module 3 | |
| fw-kivD-pSB1C3 (53 bp) | 5‘-GCAAACTATTTGCTGAACAAAATAAATCATAATACTAGTAGCGGCCGCTGCAG-3‘ |
| fw-adhA-pSB1C3 (43 bp) | 5‘-CATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| rv-alsS-pSB1C3 (65 bp) | 5‘-TTTTTGTTCTTTTGTTGCTTTTGTCAACATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| fw-pSB1C3-alsS (79 bp) | 5‘-TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGTTGACAAAAGCAACAAAAGAAC-3‘ |
| rv-ilvC-alsS) (64 bp) | 5‘-GATTCAGTGTATTGAAGTAGTTAGCCATTTTCTCCTCTTTCTAGAGAGCTTTCGTTTTCATGAG-3‘ |
| fw-alsS-ilvC) (65 bp) | 5‘-CTCATGAAAACGAAAGCTCTCTAGAAAGAGGAGAAAATGGCTAACTACTTCAATACACTGAATC-3‘ |
| rev-ilvD-ilvC (56 bp) | 5‘-GTGGCGGAACGGTACTTAGGCATTTTCTCCTCTTTTTAACCCGCAACAGCAATACG-3‘ |
| fw-ilvC-ilvD (57 bp) | 5‘-CGTATTGCTGTTGCGGGTTAAAAAGAGGAGAAAATGCCTAAGTACCGTTCCGCCAC-3‘ |
| rv-kivD-ilvD (67 bp) | 5‘-GTCTAATAGGTAATCTCCTACTGTATACATTTTCTCCTCTTTTTAACCCCCCAGTTTCGATTTATC-3‘ |
| fw-ilvD-kivD (67 bp) | 5‘-GATAAATCGAAACTGGGGGGTTAAAAAGAGGAGAAAATGTATACAGTAGGAGATTACCTATTAGAC-3‘ |
| rv-pSB1C3-kivD (74 bp) | 5‘-ACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘ |
| rv-adhA-kivD (69 bp) | 5‘-GTGTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTTTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘ |
| fw-kivD-adhA (69 bp) | 5‘-GCAAACTATTTGCTGAACAAAATAAATCATAAAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘ |
| rev-pSB1C3-adhA (80 bp) | 5‘-GTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAGTAAAATCAATGACCATTCGGCCTTC-3‘ |
| fw_pSB1C3_adhA (66 bp) | 5‘-GATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘ |
| rev_adhA_pSB1C3 (56 bp) | 5‘-GTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| fw_adhA_pSB1C3 (51 bp) | 5‘-GGCCGAATGGTCATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAG-3‘ |
