Team:Bielefeld-CeBiTec/Notebook/Primer

From 2014.igem.org

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<td width="70%">5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
<td width="70%">5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
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  <th colspan="2" align="left">Shell associated protein</th>
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<td width="30%">csoS2_sap_1_fwd (43)</td>
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<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘</td>
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<td width="30%">pSB1C3_pre_sap_1 (43 bp)</td>
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<td width="70%">5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘</td>
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<td width="30%">csoS2_sap_1_rev (38 bp)</td>
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<td width="70%">5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘</td>
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  <th colspan="2" align="left">csoS1D</th>
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<td width="30%">csoS1D_fwd (40 bp)</td>
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<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘</td>
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<td width="30%">csoS1D_rev (41 bp)</td>
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<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘</td>
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<td width="30%">pSB1C3_suf_csoS1D (43bp)</td>
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<td width="70%">5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
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<td width="30%">pSB1C3_pre_csoS1D (43 bp)</td>
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<td width="70%">5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘</td>
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Revision as of 10:13, 24 August 2014


Primerlist

Primer ID Primer sequence
Module 1
Gene synthesis
pSB1C3_pre_Fum_A (43 bp) 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_Fum_A (43 bp) 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_Fum_BCD (42 bp) 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘
pSB1C3_suf_Fum_BCD (43 bp) 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
CCM-Cassette (E. coli)
ccm_fwd (58 bp) 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘
ccm_rev (47 bp) 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘
pSB1C3_suf_ccm (50 bp) 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_ccm (28 bp) 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘
Cytochrom C (Geobacter sulfurreducens)
pccH_fwd (55 bp) 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘
pccH_rev (40 bp) 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘
pSB1C3_pre_pccH (58 bp) 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_pccH (43 bp) 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘
??? (Julian)
T7_frd_Ec_fwd (64 bp) 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘
T7_frd_Ec_rev (44 bp) 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘
pSB1C3_frd_pre (45 bp) 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘
pSB1C3_frd_suf (47 bp) 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pR6K_dcuB_short (22 bp) 5‘-AATTAACCCTCACTAAAGGGCG-3‘
Module 2
Carbonic anhydrase
csoS3_can_fwd (50 bp) 5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘
pSB1C3_pre_can (46 bp) 5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘
csoS3_can_rev (41 bp) 5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘
pSB1C3_suf_can (46 bp) 5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘
Shell associated protein
csoS2_sap_1_fwd (43) 5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘
pSB1C3_pre_sap_1 (43 bp) 5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘
csoS2_sap_1_rev (38 bp) 5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘
csoS1D
csoS1D_fwd (40 bp) 5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘
csoS1D_rev (41 bp) 5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘
pSB1C3_suf_csoS1D (43bp) 5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_csoS1D (43 bp) 5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘

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