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Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
(Difference between revisions)
| Line 79: | Line 79: | ||
<td width="30%">pSB1C3_suf_pccH (43 bp)</td> | <td width="30%">pSB1C3_suf_pccH (43 bp)</td> | ||
<td width="70%">5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
| + | </tr> | ||
| + | <tr bgcolor="#D8D8D8"> | ||
| + | <th colspan="2" align="left">??? (Julian)</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">T7_frd_Ec_fwd (64 bp)</td> | ||
| + | <td width="70%">5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">T7_frd_Ec_rev (44 bp)</td> | ||
| + | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pSB1C3_frd_pre (45 bp)</td> | ||
| + | <td width="70%">5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pSB1C3_frd_suf (47 bp)</td> | ||
| + | <td width="70%">5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pR6K_dcuB_short (22 bp)</td> | ||
| + | <td width="70%">5‘-AATTAACCCTCACTAAAGGGCG-3‘</td> | ||
</tr> | </tr> | ||
Revision as of 17:21, 22 August 2014
Primerlist
| Primer ID | Primer sequence |
|---|---|
| Module 1 | |
| Gene synthesis | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| CCM-Cassette (E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
| Cytochrom C (Geobacter sulfurreducens) | |
| pccH_fwd (55 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘ |
| pccH_rev (40 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘ |
| pSB1C3_pre_pccH (58 bp) | 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_pccH (43 bp) | 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| ??? (Julian) | |
| T7_frd_Ec_fwd (64 bp) | 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘ |
| T7_frd_Ec_rev (44 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘ |
| pSB1C3_frd_pre (45 bp) | 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘ |
| pSB1C3_frd_suf (47 bp) | 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pR6K_dcuB_short (22 bp) | 5‘-AATTAACCCTCACTAAAGGGCG-3‘ |
