<li>We made a cultivation with our construct pSB1C3_p_tac_glpX in xylose and glucose to see if there is a difference between the two media and also between induced and not induced. As a reference we used the <i>E. coli</i> wildtype. Because the culture in xylose did not grow we only could measure the OD₆₀₀ of the glucose cultures. We used 30 ml in 250 ml flasks a 37°C and 250 rpm. The induction was with IPTG. It lasted 12 hours. We made 2 biological and 2 technical replica for each culture. We took two samples (1 ml) of each biological replica for HPLC analysis that followed.</li>

       <li>We made a cultivation with our construct pSB1C3_p_tac_glpX in xylose to see if there is a difference between this cultivation and the one with glucose and also between induced and not induced. As a reference we used the <i>E. coli</i> wildtype. We used 30 ml in 250 ml flasks a 37°C and 250 rpm. The induction was with IPTG. It lasted 15 hours. We made 2 biological and 2 technical replica for each culture. We took two samples (1 ml) of each biological replica for HPLC analysis that followed.</li>

       <li>We tried to prove that the carboxysome we built can form the micro compartiment for the CO₂ fixation. Therefore we measured the GFP fluorescence signal with the GloMax of <a href="" target="_blank">Promega</a>. We took 1 ml of the culture, washed it with 1 ml 1x PBS buffer and resuspend it in 600 µl 1x PBS buffer. For the measurement we used 200 µl for each technical replica in a 96 plate (black). The given protocol for GFP fluorescence was used. <br> Additionally we examined the samples under a microscope. Herefore we prepared some object plates with 1% agarose for a fixation of the cells. 5-10 µl were used. We made pictures with two different microscopes, a Leica XXXX and a structured illumination microscope (DeltaVision|OMXv4.0 BLAZE, GE Healthcare).</li>