Team:Bielefeld-CeBiTec

From 2014.igem.org

(Difference between revisions)
 
(41 intermediate revisions not shown)
Line 1: Line 1:
-
{{Template:Team:Bielefeld-CeBiTec/CSS/main.css}}
+
{{Template:Team:Bielefeld-CeBiTec/igem.css}}
-
{{Template:Team:Bielefeld-CeBiTec/Header}}
+
{{Template:Team:Bielefeld-CeBiTec/main.css}}
 +
{{Template:Team:Bielefeld-CeBiTec/basic.tmpl}}
<html>
<html>
-
 
-
<style>
 
-
td {
 
-
    font-size:18px
 
-
}
 
-
</style>
 
-
 
-
<!--<table width="70%" align="center">-->
 
-
 
-
<!--navigation menu -->
 
-
<td align="center" colspan="3">
 
-
 
-
<!--
 
-
<table  width="1000px">
 
-
<tr heigth="15px"></tr>
 
-
<tr heigth="75px">
 
-
-->
 
<!-- automatisches Setzen des Projektüberblicks-->
<!-- automatisches Setzen des Projektüberblicks-->
<style>
<style>
#project_overview{
#project_overview{
-
float:left;
+
  float:left;
}
}
Line 30: Line 14:
#moduleX{
#moduleX{
}
}
 +
#module1{
#module1{
-
float:left;
+
  float:left;
-
position:relative;
+
  position:relative;
-
left:-170px;
+
  left:-170px;
}
}
-
div#fullpicture{
+
#fullpicture{
     margin-left:auto;
     margin-left:auto;
     margin-right:auto;
     margin-right:auto;
Line 44: Line 29:
     height:400px;
     height:400px;
}
}
-
div#fullpicture1{
+
#fullpicture1{
     background-image: url(https://static.igem.org/mediawiki/2014/6/61/Bielefeld-CeBiTec_14-06-08_Project_Module_1.png);
     background-image: url(https://static.igem.org/mediawiki/2014/6/61/Bielefeld-CeBiTec_14-06-08_Project_Module_1.png);
     background-size: 100% 100%;
     background-size: 100% 100%;
Line 51: Line 36:
     width:446px;
     width:446px;
}
}
-
div#fullpicture1:hover{
+
#fullpicture1:hover{
     background-image:url(https://static.igem.org/mediawiki/2014/d/d3/Bielefeld-CeBiTec_2014-06-04_Modul1.png);
     background-image:url(https://static.igem.org/mediawiki/2014/d/d3/Bielefeld-CeBiTec_2014-06-04_Modul1.png);
}
}
-
div#fullpicture2{
+
#fullpicture2{
     background-image: url(https://static.igem.org/mediawiki/2014/f/f6/Bielefeld-CeBiTec_14-06-08_Project_Space_1.png);
     background-image: url(https://static.igem.org/mediawiki/2014/f/f6/Bielefeld-CeBiTec_14-06-08_Project_Space_1.png);
     background-size: 100% 100%;  
     background-size: 100% 100%;  
Line 62: Line 47:
     width:158px;     
     width:158px;     
}
}
-
div#fullpicture3{
+
#fullpicture3{
     background-image: url(https://static.igem.org/mediawiki/2014/0/03/Bielefeld-CeBiTec_14-06-08_Project_Module_2.png);
     background-image: url(https://static.igem.org/mediawiki/2014/0/03/Bielefeld-CeBiTec_14-06-08_Project_Module_2.png);
     background-size:100% 100%;  
     background-size:100% 100%;  
Line 70: Line 55:
     width:330px;  
     width:330px;  
}
}
-
div#fullpicture3:hover{
+
#fullpicture3:hover{
     background-image:url(https://static.igem.org/mediawiki/2014/b/bb/Bielefeld-CeBiTec_2014-06-07_Modul2.png);
     background-image:url(https://static.igem.org/mediawiki/2014/b/bb/Bielefeld-CeBiTec_2014-06-07_Modul2.png);
}
}
-
div#fullpicture4{
+
#fullpicture4{
     background-image: url(https://static.igem.org/mediawiki/2014/9/9c/Bielefeld-CeBiTec_14-06-08_Project_Space_2.png);
     background-image: url(https://static.igem.org/mediawiki/2014/9/9c/Bielefeld-CeBiTec_14-06-08_Project_Space_2.png);
     background-size:100% 100%;
     background-size:100% 100%;
Line 81: Line 66:
     width:196px;  
     width:196px;  
}
}
-
div#fullpicture5{
+
#fullpicture5{
     background-image: url(https://static.igem.org/mediawiki/2014/b/b3/Bielefeld-CeBiTec_14-06-08_Project_Module_3.png);
     background-image: url(https://static.igem.org/mediawiki/2014/b/b3/Bielefeld-CeBiTec_14-06-08_Project_Module_3.png);
     background-size:100% 100%;  
     background-size:100% 100%;  
Line 89: Line 74:
     width:209px;  
     width:209px;  
}
}
-
div#fullpicture5:hover{
+
#fullpicture5:hover{
     background-image:url(https://static.igem.org/mediawiki/2014/7/75/Bielefeld-CeBiTec_2014-06-07_Modul3.png);
     background-image:url(https://static.igem.org/mediawiki/2014/7/75/Bielefeld-CeBiTec_2014-06-07_Modul3.png);
}
}
Line 96: Line 81:
<body>
<body>
-
<br>
 
-
<h1>Welcome to our project </h1>
 
-
<!--div id="fullpicture1"><img src="https://static.igem.org/mediawiki/2014/6/61/Bielefeld-CeBiTec_14-06-08_Project_Module_1.png" height="400px";></div>
+
<h1>Welcome to our project </h1>
-
<div id="fullpicture2"><img src="https://static.igem.org/mediawiki/2014/f/f6/Bielefeld-CeBiTec_14-06-08_Project_Space_1.png" height="400px";></div>
+
<table style="background-color:transparent;">
-
<div id="fullpicture3"><img src="https://static.igem.org/mediawiki/2014/0/03/Bielefeld-CeBiTec_14-06-08_Project_Module_2.png" height="400px";></div>
+
<tr>
-
<div id="fullpicture4"><img src="https://static.igem.org/mediawiki/2014/9/9c/Bielefeld-CeBiTec_14-06-08_Project_Space_2.png" height="400px";></div>
+
<td><!--modul 1-->
-
<div id="fullpicture5"><img src="https://static.igem.org/mediawiki/2014/b/b3/Bielefeld-CeBiTec_14-06-08_Project_Module_3.png" height="400px";></div-->
+
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><img src="https://static.igem.org/mediawiki/2014/d/d2/Bielefeld-CeBiTec_14-10-16_Mediator_NeutralRed.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><b>Results of module 1 - rMFC</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--modul 2-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><img src="https://static.igem.org/mediawiki/2014/c/c7/Bielefeld-CeBiTec_2014-10-12_Carboxysom.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><b>Results of module 2 - CO<sub>2</sub> fixation</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--modul 3-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><img src="https://static.igem.org/mediawiki/2014/1/11/Bielefeld-CeBiTec_14-10-17_IsobutProd_37.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><b>Results of module 3 - Isobutanol production</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--biosafety-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/7b/Bielefeld-CeBiTec_2014-10-13_Biosafety_Selection.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank"><b>Results of our biosafety</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--synenergene-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/51/Bielefeld-CeBiTec_2014-08-31_Fig._4_-_Szen_Windkraft.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank"><b>SYNENERGENE/ Applications</b></a></font>
 +
    </div>
 +
</td>
 +
</tr>
 +
</table>
 +
<div class="element">
 +
<div id="text">
 +
<h6>Our project</h6>
 +
<p>
 +
Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in <i>Escherichia.&nbsp;coli</i> for carbon dioxide fixation under aerobic conditions. We further engineered <i>E.&nbsp;coli</i> to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from <i>E.&nbsp;coli</i>, <i>Bacillus&nbsp;subtilis</i> and <i>Lactococcus&nbsp;lactis</i>.
 +
</p><br>
 +
</div>
 +
</div>
<!--Zusammengesetzte Projektabbildung-->
<!--Zusammengesetzte Projektabbildung-->
-
<div id="fullpicture">
+
<div class="element">
-
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module1"style="color:#000000">
+
  <div id="fullpicture">
-
  <div id="fullpicture1">
+
  <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC"style="color:#000000">
 +
      <div id="fullpicture1">
 +
        <p class="ribbon"></p>
 +
      </div>
 +
  </a>
 +
  <div id="fullpicture2">
       <p class="ribbon"></p>
       <p class="ribbon"></p>
   </div>  
   </div>  
-
</a>
+
   <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/CO2-fixation"style="color:#000000">
-
<div id="fullpicture2">
+
      <div id="fullpicture3">
-
   <p class="ribbon"></p>
+
        <p class="ribbon"></p>
-
</div>
+
      </div>
-
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module2"style="color:#000000">
+
  </a>
-
  <div id="fullpicture3">
+
  <div id="fullpicture4">
       <p class="ribbon"></p>
       <p class="ribbon"></p>
   </div>  
   </div>  
-
</a>
+
   <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Isobutanol"style="color:#000000">
-
<div id="fullpicture4">
+
      <div id="fullpicture5">
-
   <p class="ribbon"></p>
+
        <p class="ribbon"></p>
-
</div>
+
      </div>  
-
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module3"style="color:#000000">
+
  </a>
-
  <div id="fullpicture5">
+
  </div>
-
      <p class="ribbon"></p>
+
-
  </div>  
+
-
</a>
+
</div>
</div>
-
 
+
<div class="element">
-
<br>
+
<div id="text">
-
<br>
+
<h6>Achievements</h6>
-
<br>
+
<p>
-
 
+
<table style="background-color:transparent; cellspacing=3;">
-
<!--gesamte Projektübersicht-Bild-->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">rMFC</a></td></tr>
-
<!--
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">flow cell reactor</a></td></tr>
-
<tbody>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC/Mediators" target="_blank">electrophilic <i>E.coli</i> strain</a></td></tr>
-
<td><div id="project_overview"><img src="https://static.igem.org/mediawiki/2014/6/63/Bielefeld-CeBiTec_14-06-01_projekt_overview.png" width="950px" usemap="#Projekt" ;></div></td>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Successful <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Pathway" target="_blank">isobutanol production</a></td></tr>
-
-->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of a functional microcompartment, the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a></td></tr>
-
<!--Modul1-Bild-->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Genome integration of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank">OprF</a></td></tr>
-
<!--
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Functional <i>in-vitro</i> assay of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target"=_blank">RuBisCo</a></td></tr>
-
<td><div id="module1"><img src="https://static.igem.org/mediawiki/2014/d/d3/Bielefeld-CeBiTec_2014-06-04_Modul1.png" height="400px";></div></td>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Development of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank">antibiotic-free selection system</a></td></tr>
-
</tbody>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
-->
+
Several <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice" target="_blank">human practice projects</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
 
+
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank">SYNENERGENE cooperation</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
<map name="Projekt">
+
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Wikisession" target="_blank">Help another team</a></td></tr>
-
 
+
</table>
-
<!-- Link project 1 -->
+
</p>
-
<area id="m1" shape="poly" coords="0,0, 440,0, 390,160, 410,200, 365,400, 0,400"
+
</div>
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module1">
+
</div>
-
 
+
-
<!-- Link to project 2 -->
+
-
<area id="m2" shape="poly" coords="515,0, 725,0, 670,210, 745,310, 760,400, 440,400, 480,205, 460,165"
+
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module2">
+
-
 
+
-
<!-- Link to project 3 -->
+
-
<area id="m3" shape="poly" coords="780,0, 935,0, 935,400, 825,400, 785,260, 725,220"
+
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module3">
+
</body>
</body>
-
 
-
</tr>
 
</html>
</html>

Latest revision as of 14:44, 28 November 2014

Welcome to our project

Our project

Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in Escherichia. coli for carbon dioxide fixation under aerobic conditions. We further engineered E. coli to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from E. coli, Bacillus subtilis and Lactococcus lactis.


Achievements

Design and construction of a rMFC
Design and construction of a flow cell reactor
Construction of an electrophilic E.coli strain
Successful isobutanol production
Construction of a functional microcompartment, the carboxysome
Genome integration of OprF
Functional in-vitro assay of RuBisCo
Development of an antibiotic-free selection system
Several human practice projects
SYNENERGENE cooperation
Help another team