Team:Aachen/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
(→Safety)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
__NOTOC__
 +
{{Team:Aachen/Header}}
 +
[[File:Aachen_14-10-13_Pathogen_Cell_iNB.png|right|150px]]
-
{{CSS/Main}}
+
= Safety =
 +
Our iGEM team is committed to consider all aspects of the entire project, especially biosafety. For this project, two biosafety aspects have to be covered. On the one hand, we are using ''E. coli'' as '''genetically modified organism''', and on the other hand, we are detecting ''Pseudomonas aeruginosa'', an '''opportunistic human pathogen'''. It infects people suffering from cystic fibrosis or immunodeficiency as well as severe burns or open wounds. When sampling ''P. aeruginosa'', we should prevent proliferation and spread of the bacterium. For ''E. coli'', we have to take care of biological containment of a genetically modified organism.
 +
In general, we developed and designed the measurement device as '''closed system''' for a better safety handling. This way, neither the sampled pathogens nor the genetically modified sensor cells can escape our biosensor unit. For the detection, we are using one-time usage sampling and sensor chips which can be disposed of after '''autoclaving or irradation''' with strong UV light. Moreover, the electronic components are in a separate compartment and inaccessible for the user, preventing electric shock or other injuries.
-
<html>
+
<center>
 +
{{Team:Aachen/Figure|Aachen_Security_WatsOn.png|title=Biosafety level for ''WatsOn''|subtitle=Don't forget to use ''WatsOn'' only in laboratoris with the biosafty standard 1|width=500px}}
 +
</center>
-
<!--main content -->
+
To detect ''P. aeruginosa'', a sampling agar chip is slightly pressed against the solid surface to be tested. This sampling chip is put on the sensor chip and both are placed in a petri dish which is closed thoroughly. Thereby, the cells have no contact with the device during or after the measurement. However, the sensor chips must be handled in '''S1 environments only''' since they contain genetically modified ''E. coli''. Afterwards, both chips can be autoclaved and disposed. The whole lining of the measurement device is built from plastic so that it can be disinfected easily.
-
<table width="70%" align="center">
+
<!-- Afterwards, the sampling chip is immediately introduced into our measurement device and will not be removed until the detection is finished and the chips have been disinfected. The sensor chips must be handled in '''S1 environments only''' since they contain genetically modified ''E. coli''. However, once introduced into the measurement device, the sensor chips, too, will not be removed before disinfection. The living cells inside the measurement device are effectively killed after a measurement by '''using desinfectants''' such as Bacillol. For this procedure, the drawer of the measurement device is opened and Bacillol is poured over the sampling and sensor chips. -->  
 +
To '''simulate the worst case scenario''', we did replica plating of some exemplary sensor chips. In three experiments, we got an arithmetic mean of five colonies which were picked up. From that we concluded that the '''risk of infection is really low''' even if the measurement device and chips are not handled properly.
-
<!--welcome box -->
+
For further analysis of our project from a safety perspective, please view our [https://igem.org/Safety/Safety_Form?team_id=1319 safety form].
-
<tr>
+
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
-
<br>
+
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Aachen/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
-
</td>
+
-
</tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
 
-
<!-- end welcome box -->
 
-
<tr>
 
-
<!--navigation menu -->
+
{{Team:Aachen/Footer}}
-
<td align="center" colspan="3">
+
-
 
+
-
<table  width="100%">
+
-
<tr heigth="15px"></tr>
+
-
<tr heigth="75px">
+
-
 
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Aachen"style="color:#000000">Home </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Aachen/Team"style="color:#000000"> Team </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Aachen"style="color:#000000"> Official Team Profile </a></td>
+
-
 
+
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Aachen/Project"style="color:#000000"> Project</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Aachen/Parts"style="color:#000000"> Parts</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Aachen/Modeling"style="color:#000000"> Modeling</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Aachen/Notebook"style="color:#000000"> Notebook</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Aachen/Safety"style=" color:#000000"> Safety </a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Aachen/Attributions"style="color:#000000"> Attributions </a></td>
+
-
 
+
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
-
</tr>
+
-
</table>
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
</tr>
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--safety content-->
+
-
<tr><td > <h3> Welcome! </h3></td>
+
-
<td ></td >
+
-
<td > <h3> Timeline</h3></td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
+
-
 
+
-
 
+
-
 
+
-
<br>
+
-
<h3> Edit this page!</h3>
+
-
<p>
+
-
Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
+
-
<ul>
+
-
<li> <a href=" ">Learn about lab Safety for Today</a></li>
+
-
<li> <a href="">Learn about Safety for the future of your project.</a></li>
+
-
</ul>
+
-
-->
+
-
</p>
+
-
 
+
-
<h3> Your Lab </h3>
+
-
 
+
-
 
+
-
<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
+
-
<!--
+
-
<gallery>
+
-
Image:Example2_Lab_1.png|The building our lab is in!
+
-
Image:Example2_Lab_2.png|The inside of our lab!
+
-
Image:Example2_Lab_3.png|Team Member 3 doing an experiment
+
-
Image:Example2_Lab_4.png|Working in biosafety cabinets
+
-
Image:Example2_Lab_5.png|Team all gloved up and ready for work!
+
-
Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
+
-
Image:Example2_Lab_7.png|We decorated this part of our lab
+
-
Image:Example2_Lab_8.png|Whatever else you want
+
-
</gallery>-->
+
-
 
+
-
</td>
+
-
<td></td>
+
-
 
+
-
 
+
-
<td width="45%"  valign="top">
+
-
 
+
-
 
+
-
<ul>
+
-
<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
+
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
+
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
+
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
+
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
+
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
+
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
+
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
+
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
+
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
+
-
 
+
-
</ul>
+
-
</td>
+
-
 
+
-
</tr>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<tr>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</table>
+
-
</html>
+

Latest revision as of 03:51, 18 October 2014

Aachen 14-10-13 Pathogen Cell iNB.png

Safety

Our iGEM team is committed to consider all aspects of the entire project, especially biosafety. For this project, two biosafety aspects have to be covered. On the one hand, we are using E. coli as genetically modified organism, and on the other hand, we are detecting Pseudomonas aeruginosa, an opportunistic human pathogen. It infects people suffering from cystic fibrosis or immunodeficiency as well as severe burns or open wounds. When sampling P. aeruginosa, we should prevent proliferation and spread of the bacterium. For E. coli, we have to take care of biological containment of a genetically modified organism.

In general, we developed and designed the measurement device as closed system for a better safety handling. This way, neither the sampled pathogens nor the genetically modified sensor cells can escape our biosensor unit. For the detection, we are using one-time usage sampling and sensor chips which can be disposed of after autoclaving or irradation with strong UV light. Moreover, the electronic components are in a separate compartment and inaccessible for the user, preventing electric shock or other injuries.

Aachen Security WatsOn.png
Biosafety level for WatsOn
Don't forget to use WatsOn only in laboratoris with the biosafty standard 1

To detect P. aeruginosa, a sampling agar chip is slightly pressed against the solid surface to be tested. This sampling chip is put on the sensor chip and both are placed in a petri dish which is closed thoroughly. Thereby, the cells have no contact with the device during or after the measurement. However, the sensor chips must be handled in S1 environments only since they contain genetically modified E. coli. Afterwards, both chips can be autoclaved and disposed. The whole lining of the measurement device is built from plastic so that it can be disinfected easily.

To simulate the worst case scenario, we did replica plating of some exemplary sensor chips. In three experiments, we got an arithmetic mean of five colonies which were picked up. From that we concluded that the risk of infection is really low even if the measurement device and chips are not handled properly.

For further analysis of our project from a safety perspective, please view our safety form.


Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Safety"