Team:Aachen/Project/FRET Reporter
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This approach has two advantages: | This approach has two advantages: | ||
* When ''Pseudomonas aeruginosa'' is detected by our cells, the reporter protein is already expressed and only waits to be cleaved off the REACh quencher. The cleavage reaction catalyzed by the TEV protease is a faster process than expression and correct folding of GFP. This way we hope for an earlier response by our sensor cells. | * When ''Pseudomonas aeruginosa'' is detected by our cells, the reporter protein is already expressed and only waits to be cleaved off the REACh quencher. The cleavage reaction catalyzed by the TEV protease is a faster process than expression and correct folding of GFP. This way we hope for an earlier response by our sensor cells. | ||
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* While a certain concentration of homoserine lactone will produce the same number of gene read-outs, one TEV protease can cleave many GFP_REACh constructs. Through the cleavage step we therefore introduce an amplification step into our system. With the TEV protease, we will be able to produce a much stronger signal in a short time interval. | * While a certain concentration of homoserine lactone will produce the same number of gene read-outs, one TEV protease can cleave many GFP_REACh constructs. Through the cleavage step we therefore introduce an amplification step into our system. With the TEV protease, we will be able to produce a much stronger signal in a short time interval. | ||
{{Team:Aachen/Footer}} | {{Team:Aachen/Footer}} |
Revision as of 13:48, 6 October 2014
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