Team:Aachen/Project/FRET Reporter
From 2014.igem.org
(→The FRET (Förster Resonance Energy Transfer) System) |
(→A Fluorescence Answer Faster Than Expression) |
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Instead of the traditional approach, we '''constitutively express our reporter gene in its quenched form'''. As GFP_REACh fusion protein, fluorescence is suppressed. Our biosensor gives a response when homoserine lactones of ''Pseudomonas aeruginosa'' are taken up by our sensor cells where the '''autoinducer activates the expression of the TEV protease''' by binding to the LasI promoter in front of the protease gene. | Instead of the traditional approach, we '''constitutively express our reporter gene in its quenched form'''. As GFP_REACh fusion protein, fluorescence is suppressed. Our biosensor gives a response when homoserine lactones of ''Pseudomonas aeruginosa'' are taken up by our sensor cells where the '''autoinducer activates the expression of the TEV protease''' by binding to the LasI promoter in front of the protease gene. | ||
- | {{Team:Aachen/Figure|Aachen_REACh_approach.png|title=Our novel biosensor approach|subtitle=Expression of the TEV protease is induced by HSL. The protease cleaves the GFP-REACh fusion protein to elecit a fluorescence response.|width= | + | {{Team:Aachen/Figure|Aachen_REACh_approach.png|title=Our novel biosensor approach|subtitle=Expression of the TEV protease is induced by HSL. The protease cleaves the GFP-REACh fusion protein to elecit a fluorescence response.|width=900px}} |
This approach has two advantages: | This approach has two advantages: |
Revision as of 09:00, 8 October 2014
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